Assessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces

Sea lamprey (Petromyzon marinus) are invasive in the Laurentian Great Lakes, parasitize large-bodied fishes, and therefore are the focus of an international control program. However, damage caused by sea lamprey to modern day fish stocks remains uncertain because diet analysis of juvenile sea lampre...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nicholas S. Johnson, Sean A. Lewandoski, Chris Merkes
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Lamprey Diet DNA metabarcoding Great Lakes Hematophagy Gut content Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/8ffd4104ee1e4fae973bb0f5babc327b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8ffd4104ee1e4fae973bb0f5babc327b
record_format	dspace
spelling	oai:doaj.org-article:8ffd4104ee1e4fae973bb0f5babc327b2021-12-01T04:49:16ZAssessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces1470-160X10.1016/j.ecolind.2021.107605https://doaj.org/article/8ffd4104ee1e4fae973bb0f5babc327b2021-06-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X21002703https://doaj.org/toc/1470-160XSea lamprey (Petromyzon marinus) are invasive in the Laurentian Great Lakes, parasitize large-bodied fishes, and therefore are the focus of an international control program. However, damage caused by sea lamprey to modern day fish stocks remains uncertain because diet analysis of juvenile sea lamprey has been challenging; they feed on blood and are difficult to randomly sample in the lakes. Here, both challenges were addressed by showing that DNA metabarcoding of fecal material can be used to identify the diet of actively feeding juvenile sea lamprey, and can also be used to determine what non-feeding adult sea lamprey captured in streams fed on while parasitizing fish. Fecal samples from juvenile sea lamprey that were feeding on lake trout in northern Lake Huron overwhelmingly contained lake trout (Salvelinus namaycush) DNA (90%), while smaller percentages contained lake whitefish (Coregonus clupeaformis; 5%) and longnose sucker (Catostomus catostomus; 5%) DNA. Fecal samples from adult sea lamprey captured from a tributary to northern Lake Huron overwhelmingly contained longnose and white sucker DNA (Catostomus spp.; 80%), while a smaller percentage contained lake trout DNA (10%). Diet composition of adult sea lamprey sampled in the tributary (Black Mallard Creek) was more diverse than juvenile diet composition. DNA metabarcoding suggests that Catostomus spp. may be an important host fish in northern Lake Huron for sea lamprey prior to spawning. Future research could investigate how diet varies across years and lakes and the prevalence and sources of DNA contamination. Application of DNA metabarcoding for diet assessment may be practical for identifying populations of invasive sea lamprey that feed on highly valued fishes and help guide restoration of lampreys worldwide.Nicholas S. JohnsonSean A. LewandoskiChris MerkesElsevierarticleLampreyDietDNA metabarcodingGreat LakesHematophagyGut contentEcologyQH540-549.5ENEcological Indicators, Vol 125, Iss , Pp 107605- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lamprey Diet DNA metabarcoding Great Lakes Hematophagy Gut content Ecology QH540-549.5
spellingShingle	Lamprey Diet DNA metabarcoding Great Lakes Hematophagy Gut content Ecology QH540-549.5 Nicholas S. Johnson Sean A. Lewandoski Chris Merkes Assessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces
description	Sea lamprey (Petromyzon marinus) are invasive in the Laurentian Great Lakes, parasitize large-bodied fishes, and therefore are the focus of an international control program. However, damage caused by sea lamprey to modern day fish stocks remains uncertain because diet analysis of juvenile sea lamprey has been challenging; they feed on blood and are difficult to randomly sample in the lakes. Here, both challenges were addressed by showing that DNA metabarcoding of fecal material can be used to identify the diet of actively feeding juvenile sea lamprey, and can also be used to determine what non-feeding adult sea lamprey captured in streams fed on while parasitizing fish. Fecal samples from juvenile sea lamprey that were feeding on lake trout in northern Lake Huron overwhelmingly contained lake trout (Salvelinus namaycush) DNA (90%), while smaller percentages contained lake whitefish (Coregonus clupeaformis; 5%) and longnose sucker (Catostomus catostomus; 5%) DNA. Fecal samples from adult sea lamprey captured from a tributary to northern Lake Huron overwhelmingly contained longnose and white sucker DNA (Catostomus spp.; 80%), while a smaller percentage contained lake trout DNA (10%). Diet composition of adult sea lamprey sampled in the tributary (Black Mallard Creek) was more diverse than juvenile diet composition. DNA metabarcoding suggests that Catostomus spp. may be an important host fish in northern Lake Huron for sea lamprey prior to spawning. Future research could investigate how diet varies across years and lakes and the prevalence and sources of DNA contamination. Application of DNA metabarcoding for diet assessment may be practical for identifying populations of invasive sea lamprey that feed on highly valued fishes and help guide restoration of lampreys worldwide.
format	article
author	Nicholas S. Johnson Sean A. Lewandoski Chris Merkes
author_facet	Nicholas S. Johnson Sean A. Lewandoski Chris Merkes
author_sort	Nicholas S. Johnson
title	Assessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces
title_short	Assessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces
title_full	Assessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces
title_fullStr	Assessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces
title_full_unstemmed	Assessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces
title_sort	assessment of sea lamprey (petromyzon marinus) diet using dna metabarcoding of feces
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8ffd4104ee1e4fae973bb0f5babc327b
work_keys_str_mv	AT nicholassjohnson assessmentofsealampreypetromyzonmarinusdietusingdnametabarcodingoffeces AT seanalewandoski assessmentofsealampreypetromyzonmarinusdietusingdnametabarcodingoffeces AT chrismerkes assessmentofsealampreypetromyzonmarinusdietusingdnametabarcodingoffeces
_version_	1718405736271183872

Assessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces

Ejemplares similares