Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation.
Scavenger Receptor B1 (SR-B1), also known as HDL receptor, is involved in cellular cholesterol uptake. Stratum corneum (SC), the outermost layer of the skin, is composed of more than 25% cholesterol. Several reports support the view that alteration of SC lipid composition may be the cause of impaire...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/900052346926492388fc456ba05e13f1 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:900052346926492388fc456ba05e13f1 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:900052346926492388fc456ba05e13f12021-11-18T07:24:49ZCigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation.1932-620310.1371/journal.pone.0033592https://doaj.org/article/900052346926492388fc456ba05e13f12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22442701/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Scavenger Receptor B1 (SR-B1), also known as HDL receptor, is involved in cellular cholesterol uptake. Stratum corneum (SC), the outermost layer of the skin, is composed of more than 25% cholesterol. Several reports support the view that alteration of SC lipid composition may be the cause of impaired barrier function which gives rise to several skin diseases. For this reason the regulation of the genes involved in cholesterol uptake is of extreme significance for skin health. Being the first shield against external insults, the skin is exposed to several noxious substances and among these is cigarette smoke (CS), which has been recently associated with various skin pathologies. In this study we first have shown the presence of SR-B1 in murine and human skin tissue and then by using immunoblotting, immunoprecipitation, RT-PCR, and confocal microscopy we have demonstrated the translocation and the subsequent lost of SR-B1 in human keratinocytes (cell culture model) after CS exposure is driven by hydrogen peroxide (H(2)O(2)) that derives not only from the CS gas phase but mainly from the activation of cellular NADPH oxidase (NOX). This effect was reversed when the cells were pretreated with NOX inhibitors or catalase. Furthermore, CS caused the formation of SR-B1-aldheydes adducts (acrolein and 4-hydroxy-2-nonenal) and the increase of its ubiquitination, which could be one of the causes of SR-B1 loss. In conclusion, exposure to CS, through the production of H(2)O(2), induced post-translational modifications of SR-B1 with the consequence lost of the receptor and this may contribute to the skin physiology alteration as a consequence of the variation of cholesterol uptake.Claudia SticozziGiuseppe BelmonteAlessandra PecorelliBeatrice ArezziniConcetta GardiEmanuela MaioliClelia MiraccoMarzia ToscanoHenry Jay FormanGiuseppe ValacchiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33592 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Claudia Sticozzi Giuseppe Belmonte Alessandra Pecorelli Beatrice Arezzini Concetta Gardi Emanuela Maioli Clelia Miracco Marzia Toscano Henry Jay Forman Giuseppe Valacchi Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation. |
| description |
Scavenger Receptor B1 (SR-B1), also known as HDL receptor, is involved in cellular cholesterol uptake. Stratum corneum (SC), the outermost layer of the skin, is composed of more than 25% cholesterol. Several reports support the view that alteration of SC lipid composition may be the cause of impaired barrier function which gives rise to several skin diseases. For this reason the regulation of the genes involved in cholesterol uptake is of extreme significance for skin health. Being the first shield against external insults, the skin is exposed to several noxious substances and among these is cigarette smoke (CS), which has been recently associated with various skin pathologies. In this study we first have shown the presence of SR-B1 in murine and human skin tissue and then by using immunoblotting, immunoprecipitation, RT-PCR, and confocal microscopy we have demonstrated the translocation and the subsequent lost of SR-B1 in human keratinocytes (cell culture model) after CS exposure is driven by hydrogen peroxide (H(2)O(2)) that derives not only from the CS gas phase but mainly from the activation of cellular NADPH oxidase (NOX). This effect was reversed when the cells were pretreated with NOX inhibitors or catalase. Furthermore, CS caused the formation of SR-B1-aldheydes adducts (acrolein and 4-hydroxy-2-nonenal) and the increase of its ubiquitination, which could be one of the causes of SR-B1 loss. In conclusion, exposure to CS, through the production of H(2)O(2), induced post-translational modifications of SR-B1 with the consequence lost of the receptor and this may contribute to the skin physiology alteration as a consequence of the variation of cholesterol uptake. |
| format |
article |
| author |
Claudia Sticozzi Giuseppe Belmonte Alessandra Pecorelli Beatrice Arezzini Concetta Gardi Emanuela Maioli Clelia Miracco Marzia Toscano Henry Jay Forman Giuseppe Valacchi |
| author_facet |
Claudia Sticozzi Giuseppe Belmonte Alessandra Pecorelli Beatrice Arezzini Concetta Gardi Emanuela Maioli Clelia Miracco Marzia Toscano Henry Jay Forman Giuseppe Valacchi |
| author_sort |
Claudia Sticozzi |
| title |
Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation. |
| title_short |
Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation. |
| title_full |
Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation. |
| title_fullStr |
Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation. |
| title_full_unstemmed |
Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation. |
| title_sort |
cigarette smoke affects keratinocytes srb1 expression and localization via h2o2 production and hne protein adducts formation. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/900052346926492388fc456ba05e13f1 |
| work_keys_str_mv |
AT claudiasticozzi cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation AT giuseppebelmonte cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation AT alessandrapecorelli cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation AT beatricearezzini cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation AT concettagardi cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation AT emanuelamaioli cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation AT cleliamiracco cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation AT marziatoscano cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation AT henryjayforman cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation AT giuseppevalacchi cigarettesmokeaffectskeratinocytessrb1expressionandlocalizationviah2o2productionandhneproteinadductsformation |
| _version_ |
1718423487747457024 |