A Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes

Abstract A novel three dimensional blood brain barrier (BBB) platform was developed by independently supplying different types of media to separate cell types within a single device. One channel (vascular channel, VC) is connected to the inner lumen of the vascular network while the other supplies m...

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Autores principales:	Seokyoung Bang, Seung-Ryeol Lee, Jihoon Ko, Kyungmin Son, Dongha Tahk, Jungho Ahn, Changkyun Im, Noo Li Jeon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9009db61c1c64367b7ec14eb53e64c00
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spelling	oai:doaj.org-article:9009db61c1c64367b7ec14eb53e64c002021-12-02T15:06:00ZA Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes10.1038/s41598-017-07416-02045-2322https://doaj.org/article/9009db61c1c64367b7ec14eb53e64c002017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07416-0https://doaj.org/toc/2045-2322Abstract A novel three dimensional blood brain barrier (BBB) platform was developed by independently supplying different types of media to separate cell types within a single device. One channel (vascular channel, VC) is connected to the inner lumen of the vascular network while the other supplies media to the neural cells (neural channel, NC). Compared to co-cultures supplied with only one type of medium (or 1:1 mixture), best barrier properties and viability were obtained with culturing HUVECs with endothelial growth medium (EGM) and neural cells with neurobasal medium supplemented with fetal bovine serum (NBMFBS) independently. The measured vascular network permeability were comparable to reported in vivo values (20 kDa FITC-dextran, 0.45 ± 0.11 × 10−6 cm/s; 70 kDa FITC-dextran, 0.36 ± 0.05 × 10−6 cm/s) and a higher degree of neurovascular interfacing (astrocytic contact with the vascular network, GFAP-CD31 stain overlap) and presence of synapses (stained with synaptophysin). The BBB platform can dependably imitate the perivascular network morphology and synaptic structures characteristic of the NVU. This microfluidic BBB model can find applications in screening pharmaceuticals that target the brain for in neurodegenerative diseases.Seokyoung BangSeung-Ryeol LeeJihoon KoKyungmin SonDongha TahkJungho AhnChangkyun ImNoo Li JeonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seokyoung Bang Seung-Ryeol Lee Jihoon Ko Kyungmin Son Dongha Tahk Jungho Ahn Changkyun Im Noo Li Jeon A Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes
description	Abstract A novel three dimensional blood brain barrier (BBB) platform was developed by independently supplying different types of media to separate cell types within a single device. One channel (vascular channel, VC) is connected to the inner lumen of the vascular network while the other supplies media to the neural cells (neural channel, NC). Compared to co-cultures supplied with only one type of medium (or 1:1 mixture), best barrier properties and viability were obtained with culturing HUVECs with endothelial growth medium (EGM) and neural cells with neurobasal medium supplemented with fetal bovine serum (NBMFBS) independently. The measured vascular network permeability were comparable to reported in vivo values (20 kDa FITC-dextran, 0.45 ± 0.11 × 10−6 cm/s; 70 kDa FITC-dextran, 0.36 ± 0.05 × 10−6 cm/s) and a higher degree of neurovascular interfacing (astrocytic contact with the vascular network, GFAP-CD31 stain overlap) and presence of synapses (stained with synaptophysin). The BBB platform can dependably imitate the perivascular network morphology and synaptic structures characteristic of the NVU. This microfluidic BBB model can find applications in screening pharmaceuticals that target the brain for in neurodegenerative diseases.
format	article
author	Seokyoung Bang Seung-Ryeol Lee Jihoon Ko Kyungmin Son Dongha Tahk Jungho Ahn Changkyun Im Noo Li Jeon
author_facet	Seokyoung Bang Seung-Ryeol Lee Jihoon Ko Kyungmin Son Dongha Tahk Jungho Ahn Changkyun Im Noo Li Jeon
author_sort	Seokyoung Bang
title	A Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes
title_short	A Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes
title_full	A Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes
title_fullStr	A Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes
title_full_unstemmed	A Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes
title_sort	low permeability microfluidic blood-brain barrier platform with direct contact between perfusable vascular network and astrocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9009db61c1c64367b7ec14eb53e64c00
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A Low Permeability Microfluidic Blood-Brain Barrier Platform with Direct Contact between Perfusable Vascular Network and Astrocytes

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