Differential spatial expression and subcellular localization of CtBP family members in rodent brain.

C-terminal binding proteins (CtBPs) are well-characterized nuclear transcriptional co-regulators. In addition, cytoplasmic functions were discovered for these ubiquitously expressed proteins. These include the involvement of the isoform CtBP1-S/BARS50 in cellular membrane-trafficking processes and a...

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Autores principales:	Diana Hübler, Marija Rankovic, Karin Richter, Vesna Lazarevic, Wilko D Altrock, Klaus-Dieter Fischer, Eckart D Gundelfinger, Anna Fejtova
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/901804e9500d43b08b6e317e3105cde5
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spelling	oai:doaj.org-article:901804e9500d43b08b6e317e3105cde52021-11-18T07:14:36ZDifferential spatial expression and subcellular localization of CtBP family members in rodent brain.1932-620310.1371/journal.pone.0039710https://doaj.org/article/901804e9500d43b08b6e317e3105cde52012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22745816/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203C-terminal binding proteins (CtBPs) are well-characterized nuclear transcriptional co-regulators. In addition, cytoplasmic functions were discovered for these ubiquitously expressed proteins. These include the involvement of the isoform CtBP1-S/BARS50 in cellular membrane-trafficking processes and a role of the isoform RIBEYE as molecular scaffolds in ribbons, the presynaptic specializations of sensory synapses. CtBPs were suggested to regulate neuronal differentiation and they were implied in the control of gene expression during epileptogenesis. However, the expression patterns of CtBP family members in specific brain areas and their subcellular localizations in neurons in situ are largely unknown. Here, we performed comprehensive assessment of the expression of CtBP1 and CtBP2 in mouse brain at the microscopic and the ultra-structural levels using specific antibodies. We quantified and compared expression levels of both CtBPs in biochemically isolated brain fractions containing cellular nuclei or synaptic compartment. Our study demonstrates differential regional and subcellular expression patterns for the two CtBP family members in brain and reveals a previously unknown synaptic localization for CtBP2 in particular brain regions. Finally, we propose a mechanism of differential synapto-nuclear targeting of its splice variants CtBP2-S and CtBP2-L in neurons.Diana HüblerMarija RankovicKarin RichterVesna LazarevicWilko D AltrockKlaus-Dieter FischerEckart D GundelfingerAnna FejtovaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39710 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diana Hübler Marija Rankovic Karin Richter Vesna Lazarevic Wilko D Altrock Klaus-Dieter Fischer Eckart D Gundelfinger Anna Fejtova Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
description	C-terminal binding proteins (CtBPs) are well-characterized nuclear transcriptional co-regulators. In addition, cytoplasmic functions were discovered for these ubiquitously expressed proteins. These include the involvement of the isoform CtBP1-S/BARS50 in cellular membrane-trafficking processes and a role of the isoform RIBEYE as molecular scaffolds in ribbons, the presynaptic specializations of sensory synapses. CtBPs were suggested to regulate neuronal differentiation and they were implied in the control of gene expression during epileptogenesis. However, the expression patterns of CtBP family members in specific brain areas and their subcellular localizations in neurons in situ are largely unknown. Here, we performed comprehensive assessment of the expression of CtBP1 and CtBP2 in mouse brain at the microscopic and the ultra-structural levels using specific antibodies. We quantified and compared expression levels of both CtBPs in biochemically isolated brain fractions containing cellular nuclei or synaptic compartment. Our study demonstrates differential regional and subcellular expression patterns for the two CtBP family members in brain and reveals a previously unknown synaptic localization for CtBP2 in particular brain regions. Finally, we propose a mechanism of differential synapto-nuclear targeting of its splice variants CtBP2-S and CtBP2-L in neurons.
format	article
author	Diana Hübler Marija Rankovic Karin Richter Vesna Lazarevic Wilko D Altrock Klaus-Dieter Fischer Eckart D Gundelfinger Anna Fejtova
author_facet	Diana Hübler Marija Rankovic Karin Richter Vesna Lazarevic Wilko D Altrock Klaus-Dieter Fischer Eckart D Gundelfinger Anna Fejtova
author_sort	Diana Hübler
title	Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
title_short	Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
title_full	Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
title_fullStr	Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
title_full_unstemmed	Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
title_sort	differential spatial expression and subcellular localization of ctbp family members in rodent brain.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/901804e9500d43b08b6e317e3105cde5
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Differential spatial expression and subcellular localization of CtBP family members in rodent brain.

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