Active and Covert Infections of Cricket Iridovirus and Acheta domesticus Densovirus in Reared Gryllodes sigillatus Crickets

Interest in developing food, feed, and other useful products from farmed insects has gained remarkable momentum in the past decade. Crickets are an especially popular group of farmed insects due to their nutritional quality, ease of rearing, and utility. However, production of crickets as an emergin...

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Autores principales:	Kristin R. Duffield, John Hunt, Ben M. Sadd, Scott K. Sakaluk, Brenda Oppert, Karyna Rosario, Robert W. Behle, José L. Ramirez
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	reared crickets insects as food and feed Gryllodes sigillatus cricket viruses entomopathogenic viruses Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/90264d4cfb904ae38cfc6cdea9efd8a2
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spelling	oai:doaj.org-article:90264d4cfb904ae38cfc6cdea9efd8a22021-12-01T18:42:36ZActive and Covert Infections of Cricket Iridovirus and Acheta domesticus Densovirus in Reared Gryllodes sigillatus Crickets1664-302X10.3389/fmicb.2021.780796https://doaj.org/article/90264d4cfb904ae38cfc6cdea9efd8a22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.780796/fullhttps://doaj.org/toc/1664-302XInterest in developing food, feed, and other useful products from farmed insects has gained remarkable momentum in the past decade. Crickets are an especially popular group of farmed insects due to their nutritional quality, ease of rearing, and utility. However, production of crickets as an emerging commodity has been severely impacted by entomopathogenic infections, about which we know little. Here, we identified and characterized an unknown entomopathogen causing mass mortality in a lab-reared population of Gryllodes sigillatus crickets, a species used as an alternative to the popular Acheta domesticus due to its claimed tolerance to prevalent entomopathogenic viruses. Microdissection of sick and healthy crickets coupled with metagenomics-based identification and real-time qPCR viral quantification indicated high levels of cricket iridovirus (CrIV) in a symptomatic population, and evidence of covert CrIV infections in a healthy population. Our study also identified covert infections of Acheta domesticus densovirus (AdDNV) in both populations of G. sigillatus. These results add to the foundational research needed to better understand the pathology of mass-reared insects and ultimately develop the prevention, mitigation, and intervention strategies needed for economical production of insects as a commodity.Kristin R. DuffieldJohn HuntBen M. SaddScott K. SakalukBrenda OppertKaryna RosarioRobert W. BehleJosé L. RamirezFrontiers Media S.A.articlereared cricketsinsects as food and feedGryllodes sigillatuscricket virusesentomopathogenic virusesMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	reared crickets insects as food and feed Gryllodes sigillatus cricket viruses entomopathogenic viruses Microbiology QR1-502
spellingShingle	reared crickets insects as food and feed Gryllodes sigillatus cricket viruses entomopathogenic viruses Microbiology QR1-502 Kristin R. Duffield John Hunt Ben M. Sadd Scott K. Sakaluk Brenda Oppert Karyna Rosario Robert W. Behle José L. Ramirez Active and Covert Infections of Cricket Iridovirus and Acheta domesticus Densovirus in Reared Gryllodes sigillatus Crickets
description	Interest in developing food, feed, and other useful products from farmed insects has gained remarkable momentum in the past decade. Crickets are an especially popular group of farmed insects due to their nutritional quality, ease of rearing, and utility. However, production of crickets as an emerging commodity has been severely impacted by entomopathogenic infections, about which we know little. Here, we identified and characterized an unknown entomopathogen causing mass mortality in a lab-reared population of Gryllodes sigillatus crickets, a species used as an alternative to the popular Acheta domesticus due to its claimed tolerance to prevalent entomopathogenic viruses. Microdissection of sick and healthy crickets coupled with metagenomics-based identification and real-time qPCR viral quantification indicated high levels of cricket iridovirus (CrIV) in a symptomatic population, and evidence of covert CrIV infections in a healthy population. Our study also identified covert infections of Acheta domesticus densovirus (AdDNV) in both populations of G. sigillatus. These results add to the foundational research needed to better understand the pathology of mass-reared insects and ultimately develop the prevention, mitigation, and intervention strategies needed for economical production of insects as a commodity.
format	article
author	Kristin R. Duffield John Hunt Ben M. Sadd Scott K. Sakaluk Brenda Oppert Karyna Rosario Robert W. Behle José L. Ramirez
author_facet	Kristin R. Duffield John Hunt Ben M. Sadd Scott K. Sakaluk Brenda Oppert Karyna Rosario Robert W. Behle José L. Ramirez
author_sort	Kristin R. Duffield
title	Active and Covert Infections of Cricket Iridovirus and Acheta domesticus Densovirus in Reared Gryllodes sigillatus Crickets
title_short	Active and Covert Infections of Cricket Iridovirus and Acheta domesticus Densovirus in Reared Gryllodes sigillatus Crickets
title_full	Active and Covert Infections of Cricket Iridovirus and Acheta domesticus Densovirus in Reared Gryllodes sigillatus Crickets
title_fullStr	Active and Covert Infections of Cricket Iridovirus and Acheta domesticus Densovirus in Reared Gryllodes sigillatus Crickets
title_full_unstemmed	Active and Covert Infections of Cricket Iridovirus and Acheta domesticus Densovirus in Reared Gryllodes sigillatus Crickets
title_sort	active and covert infections of cricket iridovirus and acheta domesticus densovirus in reared gryllodes sigillatus crickets
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/90264d4cfb904ae38cfc6cdea9efd8a2
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Active and Covert Infections of Cricket Iridovirus and Acheta domesticus Densovirus in Reared Gryllodes sigillatus Crickets

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