Comparative Evaluations of the Pathogenesis of <named-content content-type="genus-species">Candida auris</named-content> Phenotypes and <named-content content-type="genus-species">Candida albicans</named-content> Using Clinically Relevant Murine Models of Infections

ABSTRACT The newly emerged Candida species Candida auris is associated with an exponential rise in life-threatening invasive disease in health care facilities worldwide. Unlike other species, C. auris exhibits a high level of transmissibility, multidrug resistance, and persistence in the environment...

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Autores principales:	Taissa Vila, Daniel Montelongo-Jauregui, Hussian Ahmed, Taanya Puthran, Ahmed S. Sultan, Mary Ann Jabra-Rizk
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	fungal pathogens Candida auris Candida albicans animal models biofilm formation biofilms Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9030ef22f0994386b89bd93a14949eb6
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spelling	oai:doaj.org-article:9030ef22f0994386b89bd93a14949eb62021-11-15T15:30:50ZComparative Evaluations of the Pathogenesis of <named-content content-type="genus-species">Candida auris</named-content> Phenotypes and <named-content content-type="genus-species">Candida albicans</named-content> Using Clinically Relevant Murine Models of Infections10.1128/mSphere.00760-202379-5042https://doaj.org/article/9030ef22f0994386b89bd93a14949eb62020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00760-20https://doaj.org/toc/2379-5042ABSTRACT The newly emerged Candida species Candida auris is associated with an exponential rise in life-threatening invasive disease in health care facilities worldwide. Unlike other species, C. auris exhibits a high level of transmissibility, multidrug resistance, and persistence in the environment, yet little is known about its pathogenesis largely due to limited data from animal models. Based on in vitro biofilm evaluations and confocal laser scanning microscopy, C. auris phenotypes with different biofilm-forming abilities were identified, indicating potential clinical implications. Using clinically relevant murine models of implanted catheter, oral, and intraperitoneal infections, we comparatively evaluated the host site-specific pathogenic potential of C. auris phenotypes and Candida albicans. Based on the results of microbial recovery and scanning electron microscopy analysis of explanted catheters, compared to C. albicans, C. auris more avidly adhered and formed biofilms on catheters. However, although C. auris adhered to oral tissue ex vivo, unlike C. albicans, it failed to colonize the oral cavity in vivo, as demonstrated by microbial recovery and tissue histopathology analysis. In contrast, recovery from peritoneal lavage fluid and kidneys during time course experiments demonstrated that C. auris persisted longer in the peritoneal cavity and kidneys. Although there were clear niche-specific differences in pathogenic features between C. auris and C. albicans, no significant differences were noted between the C. auris phenotypes in vivo. The combined findings highlight unique niche-specific pathogenic traits for C. auris warranting further investigations. Understanding the factors contributing to the rise of C. auris as a nosocomial pathogen is critical for controlling the spread of this species. IMPORTANCE The newly emerged Candida species C. auris has been associated with an exponential rise in invasive disease in health care facilities worldwide with a mortality rate approaching 60%. C. auris exhibits a high level of transmissibility, multidrug resistance, and persistence in hospital environments, yet little is known about its pathogenesis largely due to limited data from animal studies. We used clinically relevant murine models of infection to comparatively evaluate the host niche-specific pathogenic potential of C. auris and C. albicans. Findings demonstrated that C. auris adheres more avidly, forming robust biofilms on catheters implanted in mice. However, although C. auris adhered to oral tissue ex vivo, it failed to colonize the oral cavity in vivo. In contrast, in the intraperitoneal infection model, C. auris persisted longer in the peritoneal cavity and kidneys. Understanding the host-pathogen factors contributing to the rise of C. auris as a nosocomial pathogen is critical for controlling the spread of this species.Taissa VilaDaniel Montelongo-JaureguiHussian AhmedTaanya PuthranAhmed S. SultanMary Ann Jabra-RizkAmerican Society for Microbiologyarticlefungal pathogensCandida aurisCandida albicansanimal modelsbiofilm formationbiofilmsMicrobiologyQR1-502ENmSphere, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	fungal pathogens Candida auris Candida albicans animal models biofilm formation biofilms Microbiology QR1-502
spellingShingle	fungal pathogens Candida auris Candida albicans animal models biofilm formation biofilms Microbiology QR1-502 Taissa Vila Daniel Montelongo-Jauregui Hussian Ahmed Taanya Puthran Ahmed S. Sultan Mary Ann Jabra-Rizk Comparative Evaluations of the Pathogenesis of <named-content content-type="genus-species">Candida auris</named-content> Phenotypes and <named-content content-type="genus-species">Candida albicans</named-content> Using Clinically Relevant Murine Models of Infections
description	ABSTRACT The newly emerged Candida species Candida auris is associated with an exponential rise in life-threatening invasive disease in health care facilities worldwide. Unlike other species, C. auris exhibits a high level of transmissibility, multidrug resistance, and persistence in the environment, yet little is known about its pathogenesis largely due to limited data from animal models. Based on in vitro biofilm evaluations and confocal laser scanning microscopy, C. auris phenotypes with different biofilm-forming abilities were identified, indicating potential clinical implications. Using clinically relevant murine models of implanted catheter, oral, and intraperitoneal infections, we comparatively evaluated the host site-specific pathogenic potential of C. auris phenotypes and Candida albicans. Based on the results of microbial recovery and scanning electron microscopy analysis of explanted catheters, compared to C. albicans, C. auris more avidly adhered and formed biofilms on catheters. However, although C. auris adhered to oral tissue ex vivo, unlike C. albicans, it failed to colonize the oral cavity in vivo, as demonstrated by microbial recovery and tissue histopathology analysis. In contrast, recovery from peritoneal lavage fluid and kidneys during time course experiments demonstrated that C. auris persisted longer in the peritoneal cavity and kidneys. Although there were clear niche-specific differences in pathogenic features between C. auris and C. albicans, no significant differences were noted between the C. auris phenotypes in vivo. The combined findings highlight unique niche-specific pathogenic traits for C. auris warranting further investigations. Understanding the factors contributing to the rise of C. auris as a nosocomial pathogen is critical for controlling the spread of this species. IMPORTANCE The newly emerged Candida species C. auris has been associated with an exponential rise in invasive disease in health care facilities worldwide with a mortality rate approaching 60%. C. auris exhibits a high level of transmissibility, multidrug resistance, and persistence in hospital environments, yet little is known about its pathogenesis largely due to limited data from animal studies. We used clinically relevant murine models of infection to comparatively evaluate the host niche-specific pathogenic potential of C. auris and C. albicans. Findings demonstrated that C. auris adheres more avidly, forming robust biofilms on catheters implanted in mice. However, although C. auris adhered to oral tissue ex vivo, it failed to colonize the oral cavity in vivo. In contrast, in the intraperitoneal infection model, C. auris persisted longer in the peritoneal cavity and kidneys. Understanding the host-pathogen factors contributing to the rise of C. auris as a nosocomial pathogen is critical for controlling the spread of this species.
format	article
author	Taissa Vila Daniel Montelongo-Jauregui Hussian Ahmed Taanya Puthran Ahmed S. Sultan Mary Ann Jabra-Rizk
author_facet	Taissa Vila Daniel Montelongo-Jauregui Hussian Ahmed Taanya Puthran Ahmed S. Sultan Mary Ann Jabra-Rizk
author_sort	Taissa Vila
title	Comparative Evaluations of the Pathogenesis of <named-content content-type="genus-species">Candida auris</named-content> Phenotypes and <named-content content-type="genus-species">Candida albicans</named-content> Using Clinically Relevant Murine Models of Infections
title_short	Comparative Evaluations of the Pathogenesis of <named-content content-type="genus-species">Candida auris</named-content> Phenotypes and <named-content content-type="genus-species">Candida albicans</named-content> Using Clinically Relevant Murine Models of Infections
title_full	Comparative Evaluations of the Pathogenesis of <named-content content-type="genus-species">Candida auris</named-content> Phenotypes and <named-content content-type="genus-species">Candida albicans</named-content> Using Clinically Relevant Murine Models of Infections
title_fullStr	Comparative Evaluations of the Pathogenesis of <named-content content-type="genus-species">Candida auris</named-content> Phenotypes and <named-content content-type="genus-species">Candida albicans</named-content> Using Clinically Relevant Murine Models of Infections
title_full_unstemmed	Comparative Evaluations of the Pathogenesis of <named-content content-type="genus-species">Candida auris</named-content> Phenotypes and <named-content content-type="genus-species">Candida albicans</named-content> Using Clinically Relevant Murine Models of Infections
title_sort	comparative evaluations of the pathogenesis of <named-content content-type="genus-species">candida auris</named-content> phenotypes and <named-content content-type="genus-species">candida albicans</named-content> using clinically relevant murine models of infections
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/9030ef22f0994386b89bd93a14949eb6
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Comparative Evaluations of the Pathogenesis of <named-content content-type="genus-species">Candida auris</named-content> Phenotypes and <named-content content-type="genus-species">Candida albicans</named-content> Using Clinically Relevant Murine Models of Infections

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