Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout

Abstract Rainbow trout (Oncorhynchus mykiss) is considered a “glucose-intolerant” species. With the aim of programming trout to improve their metabolic use of dietary carbohydrates, we hypothesised that a hypoxic stimulus applied during embryogenesis could later affect glucose metabolism at the firs...

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Autores principales: Jingwei Liu, Elisabeth Plagnes-Juan, Inge Geurden, Stéphane Panserat, Lucie Marandel
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/903333cd27f841a7b234cfa66c813884
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spelling oai:doaj.org-article:903333cd27f841a7b234cfa66c8138842021-12-02T12:30:53ZExposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout10.1038/s41598-017-00458-42045-2322https://doaj.org/article/903333cd27f841a7b234cfa66c8138842017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00458-4https://doaj.org/toc/2045-2322Abstract Rainbow trout (Oncorhynchus mykiss) is considered a “glucose-intolerant” species. With the aim of programming trout to improve their metabolic use of dietary carbohydrates, we hypothesised that a hypoxic stimulus applied during embryogenesis could later affect glucose metabolism at the first-feeding stage. An acute hypoxic stimulus (2.5 or 5.0 mg·L−1 O2) was applied for 24 h to non-hatched embryos or early hatched alevins followed by a challenge test with a high carbohydrate diet at first-feeding. The effectiveness of the early hypoxic stimulus was confirmed by the induction of oxygen-sensitive markers such as egln3. At first-feeding, trout previously subjected to the 2.5 mg·L−1 O2 hypoxia displayed a strong induction of glycolytic and glucose transport genes, whereas these glucose metabolism-related genes were affected much less in trout subjected to the less severe (5.0 mg·L−1 O2) hypoxia. Our results demonstrate that an acute hypoxic stimulus during early development can affect glucose metabolism in trout at first-feeding.Jingwei LiuElisabeth Plagnes-JuanInge GeurdenStéphane PanseratLucie MarandelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jingwei Liu
Elisabeth Plagnes-Juan
Inge Geurden
Stéphane Panserat
Lucie Marandel
Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout
description Abstract Rainbow trout (Oncorhynchus mykiss) is considered a “glucose-intolerant” species. With the aim of programming trout to improve their metabolic use of dietary carbohydrates, we hypothesised that a hypoxic stimulus applied during embryogenesis could later affect glucose metabolism at the first-feeding stage. An acute hypoxic stimulus (2.5 or 5.0 mg·L−1 O2) was applied for 24 h to non-hatched embryos or early hatched alevins followed by a challenge test with a high carbohydrate diet at first-feeding. The effectiveness of the early hypoxic stimulus was confirmed by the induction of oxygen-sensitive markers such as egln3. At first-feeding, trout previously subjected to the 2.5 mg·L−1 O2 hypoxia displayed a strong induction of glycolytic and glucose transport genes, whereas these glucose metabolism-related genes were affected much less in trout subjected to the less severe (5.0 mg·L−1 O2) hypoxia. Our results demonstrate that an acute hypoxic stimulus during early development can affect glucose metabolism in trout at first-feeding.
format article
author Jingwei Liu
Elisabeth Plagnes-Juan
Inge Geurden
Stéphane Panserat
Lucie Marandel
author_facet Jingwei Liu
Elisabeth Plagnes-Juan
Inge Geurden
Stéphane Panserat
Lucie Marandel
author_sort Jingwei Liu
title Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout
title_short Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout
title_full Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout
title_fullStr Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout
title_full_unstemmed Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout
title_sort exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/903333cd27f841a7b234cfa66c813884
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AT stephanepanserat exposuretoanacutehypoxicstimulusduringearlylifeaffectstheexpressionofglucosemetabolismrelatedgenesatfirstfeedingintrout
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