Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry

Camila G Andrade,1 Paulo E Cabral Filho,2 Denise PL Tenório,3 Beate S Santos,4 Eduardo IC Beltrão,1 Adriana Fontes,2 Luiz B Carvalho Jr1 1Keizo Asami Immunopathology Laboratory, 2Biophysics and Radiobiology Department, 3Fundamental Chemistry Department, 4Pharmaceutical Science...

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Autores principales:	Andrade CG, Cabral Filho PE, Tenorio DPL, Santos BS, Beltr&atilde;o EIC, Fontes A, Carvalho Jr LB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/903545208ac146aba4a66151ee71aac3
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spelling	oai:doaj.org-article:903545208ac146aba4a66151ee71aac32021-12-02T01:07:51ZEvaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry1176-91141178-2013https://doaj.org/article/903545208ac146aba4a66151ee71aac32013-12-01T00:00:00Zhttp://www.dovepress.com/evaluation-of-glycophenotype-in-breast-cancer-by-quantum-dot-lectin-hi-a15139https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Camila G Andrade,1 Paulo E Cabral Filho,2 Denise PL Tenório,3 Beate S Santos,4 Eduardo IC Beltrão,1 Adriana Fontes,2 Luiz B Carvalho Jr1 1Keizo Asami Immunopathology Laboratory, 2Biophysics and Radiobiology Department, 3Fundamental Chemistry Department, 4Pharmaceutical Science Department, Federal University of Pernambuco, Recife, Pernambuco, Brazil Abstract: Cell surface glycoconjugates play an important role in differentiation/dedifferentiation processes and lectins are employed to evaluate them by several methodologies. Fluorescent probes are considered a valuable tool because of their ability to provide a particular view, and are more detailed and sensitive in terms of cell structure and molecular content. The aim of this study was to evaluate and compare the expression and distribution of glycoconjugates in normal human breast tissue, and benign (fibroadenoma), and malignantly transformed (invasive ductal carcinoma) breast tissues. For this, we used mercaptosuccinic acid-coated Cadmium Telluride (CdTe) quantum dots (QDs) conjugated with concanavalin A (Con A) or Ulex europaeus agglutinin I (UEA I) lectins to detect α-D-glucose/mannose and L-fucose residues, respectively. The QD-lectin conjugates were evaluated by hemagglutination activity tests and carbohydrate inhibition assays, and were found to remain functional, keeping their fluorescent properties and carbohydrate recognition ability. Fluorescence images showed that different regions of breast tissue expressed particular types of carbohydrates. While the stroma was preferentially and intensely stained by QD-Con A, ductal cells were preferentially labeled by QD-UEA I. These results indicate that QD-lectin conjugates can be used as molecular probes and can help to elucidate the glycoconjugate profile in biological processes. Keywords: nanoparticles, concanavalin A, Ulex europaeus, carbohydrates, mammary tissuesAndrade CGCabral Filho PETenorio DPLSantos BSBeltr&atilde;o EICFontes ACarvalho Jr LBDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 4623-4629 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Andrade CG Cabral Filho PE Tenorio DPL Santos BS Beltr&atilde;o EIC Fontes A Carvalho Jr LB Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry
description	Camila G Andrade,1 Paulo E Cabral Filho,2 Denise PL Tenório,3 Beate S Santos,4 Eduardo IC Beltrão,1 Adriana Fontes,2 Luiz B Carvalho Jr1 1Keizo Asami Immunopathology Laboratory, 2Biophysics and Radiobiology Department, 3Fundamental Chemistry Department, 4Pharmaceutical Science Department, Federal University of Pernambuco, Recife, Pernambuco, Brazil Abstract: Cell surface glycoconjugates play an important role in differentiation/dedifferentiation processes and lectins are employed to evaluate them by several methodologies. Fluorescent probes are considered a valuable tool because of their ability to provide a particular view, and are more detailed and sensitive in terms of cell structure and molecular content. The aim of this study was to evaluate and compare the expression and distribution of glycoconjugates in normal human breast tissue, and benign (fibroadenoma), and malignantly transformed (invasive ductal carcinoma) breast tissues. For this, we used mercaptosuccinic acid-coated Cadmium Telluride (CdTe) quantum dots (QDs) conjugated with concanavalin A (Con A) or Ulex europaeus agglutinin I (UEA I) lectins to detect α-D-glucose/mannose and L-fucose residues, respectively. The QD-lectin conjugates were evaluated by hemagglutination activity tests and carbohydrate inhibition assays, and were found to remain functional, keeping their fluorescent properties and carbohydrate recognition ability. Fluorescence images showed that different regions of breast tissue expressed particular types of carbohydrates. While the stroma was preferentially and intensely stained by QD-Con A, ductal cells were preferentially labeled by QD-UEA I. These results indicate that QD-lectin conjugates can be used as molecular probes and can help to elucidate the glycoconjugate profile in biological processes. Keywords: nanoparticles, concanavalin A, Ulex europaeus, carbohydrates, mammary tissues
format	article
author	Andrade CG Cabral Filho PE Tenorio DPL Santos BS Beltr&atilde;o EIC Fontes A Carvalho Jr LB
author_facet	Andrade CG Cabral Filho PE Tenorio DPL Santos BS Beltr&atilde;o EIC Fontes A Carvalho Jr LB
author_sort	Andrade CG
title	Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry
title_short	Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry
title_full	Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry
title_fullStr	Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry
title_full_unstemmed	Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry
title_sort	evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/903545208ac146aba4a66151ee71aac3
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Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry

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