Metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide

This work presents a simple, low-cost and ready-to-use peroxide biosensor based on a metal-supported, self-assembled lipid membrane incorporated with horseradish peroxidase. The peroxide sensor showed good analytical and operational characteristics: the detection limit (as S/N = 3) was 0.083 μM and...

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Autores principales:	Christina G. Siontorou, Konstantinos N. Georgopoulos
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Lipid membrane biosensor Self-assembly Horseradish peroxidase Water monitoring Hydrogen peroxide Technology T
Acceso en línea:	https://doaj.org/article/9037c99251434822b7c2be3268d581df
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spelling	oai:doaj.org-article:9037c99251434822b7c2be3268d581df2021-11-26T04:39:00ZMetal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide2590-123010.1016/j.rineng.2021.100312https://doaj.org/article/9037c99251434822b7c2be3268d581df2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590123021001134https://doaj.org/toc/2590-1230This work presents a simple, low-cost and ready-to-use peroxide biosensor based on a metal-supported, self-assembled lipid membrane incorporated with horseradish peroxidase. The peroxide sensor showed good analytical and operational characteristics: the detection limit (as S/N = 3) was 0.083 μM and the sensitivity was 1.4071 nA per μM of analyte concentration. The sensor was stable (<5% drift) for 9 h of continuous operation. The validation in real water samples showed that the sensor could reliably detect 0.95 mM of hydrogen peroxide in tap, river and lake water. The reliability of the sensor has been also proven on commercial peroxide formulations, assayed without pretreatment. The prospects and applicability of the proposed biosensor and the underlying technology are also discussed.Christina G. SiontorouKonstantinos N. GeorgopoulosElsevierarticleLipid membrane biosensorSelf-assemblyHorseradish peroxidaseWater monitoringHydrogen peroxideTechnologyTENResults in Engineering, Vol 12, Iss , Pp 100312- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lipid membrane biosensor Self-assembly Horseradish peroxidase Water monitoring Hydrogen peroxide Technology T
spellingShingle	Lipid membrane biosensor Self-assembly Horseradish peroxidase Water monitoring Hydrogen peroxide Technology T Christina G. Siontorou Konstantinos N. Georgopoulos Metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide
description	This work presents a simple, low-cost and ready-to-use peroxide biosensor based on a metal-supported, self-assembled lipid membrane incorporated with horseradish peroxidase. The peroxide sensor showed good analytical and operational characteristics: the detection limit (as S/N = 3) was 0.083 μM and the sensitivity was 1.4071 nA per μM of analyte concentration. The sensor was stable (<5% drift) for 9 h of continuous operation. The validation in real water samples showed that the sensor could reliably detect 0.95 mM of hydrogen peroxide in tap, river and lake water. The reliability of the sensor has been also proven on commercial peroxide formulations, assayed without pretreatment. The prospects and applicability of the proposed biosensor and the underlying technology are also discussed.
format	article
author	Christina G. Siontorou Konstantinos N. Georgopoulos
author_facet	Christina G. Siontorou Konstantinos N. Georgopoulos
author_sort	Christina G. Siontorou
title	Metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide
title_short	Metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide
title_full	Metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide
title_fullStr	Metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide
title_full_unstemmed	Metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide
title_sort	metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9037c99251434822b7c2be3268d581df
work_keys_str_mv	AT christinagsiontorou metalsupportedselfassembledbilayerlipidmembraneincorporatedwithperoxidaseforthedetectionofperoxide AT konstantinosngeorgopoulos metalsupportedselfassembledbilayerlipidmembraneincorporatedwithperoxidaseforthedetectionofperoxide
_version_	1718409854221025280

Metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide

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