Chromosomal rearrangements between serotype A and D strains in Cryptococcus neoformans.

Cryptococcus neoformans is a major human pathogenic fungus that can cause meningoencephalitis in immunocompromised hosts. It contains two divergent varieties, var. grubii (serotype A) and var. neoformans (serotype D), as well as hybrids (serotype AD) between these two varieties. In this study, we in...

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Autores principales:	Sheng Sun, Jianping Xu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/903ccce892214e6e94b1508f5ab715d1
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spelling	oai:doaj.org-article:903ccce892214e6e94b1508f5ab715d12021-11-25T06:22:43ZChromosomal rearrangements between serotype A and D strains in Cryptococcus neoformans.1932-620310.1371/journal.pone.0005524https://doaj.org/article/903ccce892214e6e94b1508f5ab715d12009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19436753/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cryptococcus neoformans is a major human pathogenic fungus that can cause meningoencephalitis in immunocompromised hosts. It contains two divergent varieties, var. grubii (serotype A) and var. neoformans (serotype D), as well as hybrids (serotype AD) between these two varieties. In this study, we investigated the extent of chromosomal rearrangements between the two varieties, estimated the effects of chromosomal rearrangements on recombination frequencies, and surveyed the potential polymorphisms of the rearrangements among natural strains of the three serotypes. Through the analyses of two sequenced genomes from strains H99 (representing var. grubii) and JEC21 (representing var. neoformans), we revealed a total of 32 unambiguous chromosome rearrangements, including five translocations, nine simple inversions, and 18 complex rearrangements. Our analyses identified that overall, rearranged regions had recombination frequencies about half of those around syntenic regions. Using a direct PCR screening strategy, we examined the potential polymorphisms of 11 rearrangements among 64 natural C. neoformans strains from five countries. We found no polymorphism within var. neoformans and very limited polymorphism within var. grubii. However, strains of serotype AD showed significant polymorphism, consistent with their hybrid origins coupled with differential loss of heterozygosity. We discuss the implications of these results on the genome structure, ecology, and evolution of C. neoformans.Sheng SunJianping XuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 5, p e5524 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sheng Sun Jianping Xu Chromosomal rearrangements between serotype A and D strains in Cryptococcus neoformans.
description	Cryptococcus neoformans is a major human pathogenic fungus that can cause meningoencephalitis in immunocompromised hosts. It contains two divergent varieties, var. grubii (serotype A) and var. neoformans (serotype D), as well as hybrids (serotype AD) between these two varieties. In this study, we investigated the extent of chromosomal rearrangements between the two varieties, estimated the effects of chromosomal rearrangements on recombination frequencies, and surveyed the potential polymorphisms of the rearrangements among natural strains of the three serotypes. Through the analyses of two sequenced genomes from strains H99 (representing var. grubii) and JEC21 (representing var. neoformans), we revealed a total of 32 unambiguous chromosome rearrangements, including five translocations, nine simple inversions, and 18 complex rearrangements. Our analyses identified that overall, rearranged regions had recombination frequencies about half of those around syntenic regions. Using a direct PCR screening strategy, we examined the potential polymorphisms of 11 rearrangements among 64 natural C. neoformans strains from five countries. We found no polymorphism within var. neoformans and very limited polymorphism within var. grubii. However, strains of serotype AD showed significant polymorphism, consistent with their hybrid origins coupled with differential loss of heterozygosity. We discuss the implications of these results on the genome structure, ecology, and evolution of C. neoformans.
format	article
author	Sheng Sun Jianping Xu
author_facet	Sheng Sun Jianping Xu
author_sort	Sheng Sun
title	Chromosomal rearrangements between serotype A and D strains in Cryptococcus neoformans.
title_short	Chromosomal rearrangements between serotype A and D strains in Cryptococcus neoformans.
title_full	Chromosomal rearrangements between serotype A and D strains in Cryptococcus neoformans.
title_fullStr	Chromosomal rearrangements between serotype A and D strains in Cryptococcus neoformans.
title_full_unstemmed	Chromosomal rearrangements between serotype A and D strains in Cryptococcus neoformans.
title_sort	chromosomal rearrangements between serotype a and d strains in cryptococcus neoformans.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/903ccce892214e6e94b1508f5ab715d1
work_keys_str_mv	AT shengsun chromosomalrearrangementsbetweenserotypeaanddstrainsincryptococcusneoformans AT jianpingxu chromosomalrearrangementsbetweenserotypeaanddstrainsincryptococcusneoformans
_version_	1718413813558018048

Chromosomal rearrangements between serotype A and D strains in Cryptococcus neoformans.

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