Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections

Abstract Teleost fish skin serves as the first line of defense against pathogens. The interaction between pathogen and host skin determines the infection outcome. However, the mechanism(s) that modulate infection remain largely unknown. A proper tissue culture model that is easier to handle but can...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ru-Fang Siao, Chia-Hsuan Lin, Li-Hsuan Chen, Liang-Chun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/90468c487b1948d5b8432d9d4e7e26b5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:90468c487b1948d5b8432d9d4e7e26b5
record_format	dspace
spelling	oai:doaj.org-article:90468c487b1948d5b8432d9d4e7e26b52021-12-02T18:14:22ZEstablishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections10.1038/s41598-021-98583-82045-2322https://doaj.org/article/90468c487b1948d5b8432d9d4e7e26b52021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98583-8https://doaj.org/toc/2045-2322Abstract Teleost fish skin serves as the first line of defense against pathogens. The interaction between pathogen and host skin determines the infection outcome. However, the mechanism(s) that modulate infection remain largely unknown. A proper tissue culture model that is easier to handle but can quantitatively and qualitatively monitor infection progress may shed some lights. Here, we use striped catfish (Pangasius hypophthalmus) to establish an ex vivo skin explant tissue culture model to explore host pathogen interactions. The skin explant model resembles in vivo skin in tissue morphology, integrity, and immune functionality. Inoculation of aquatic pathogen Aeromonas hydrophila in this model induces epidermal exfoliation along with epithelial cell dissociation and inflammation. We conclude that this ex vivo skin explant model could serve as a teleost skin infection model for monitoring pathogenesis under various infection conditions. The model can also potentially be translated into a platform to study prevention and treatment of aquatic infection on the skin in aquaculture applications.Ru-Fang SiaoChia-Hsuan LinLi-Hsuan ChenLiang-Chun WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ru-Fang Siao Chia-Hsuan Lin Li-Hsuan Chen Liang-Chun Wang Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections
description	Abstract Teleost fish skin serves as the first line of defense against pathogens. The interaction between pathogen and host skin determines the infection outcome. However, the mechanism(s) that modulate infection remain largely unknown. A proper tissue culture model that is easier to handle but can quantitatively and qualitatively monitor infection progress may shed some lights. Here, we use striped catfish (Pangasius hypophthalmus) to establish an ex vivo skin explant tissue culture model to explore host pathogen interactions. The skin explant model resembles in vivo skin in tissue morphology, integrity, and immune functionality. Inoculation of aquatic pathogen Aeromonas hydrophila in this model induces epidermal exfoliation along with epithelial cell dissociation and inflammation. We conclude that this ex vivo skin explant model could serve as a teleost skin infection model for monitoring pathogenesis under various infection conditions. The model can also potentially be translated into a platform to study prevention and treatment of aquatic infection on the skin in aquaculture applications.
format	article
author	Ru-Fang Siao Chia-Hsuan Lin Li-Hsuan Chen Liang-Chun Wang
author_facet	Ru-Fang Siao Chia-Hsuan Lin Li-Hsuan Chen Liang-Chun Wang
author_sort	Ru-Fang Siao
title	Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections
title_short	Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections
title_full	Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections
title_fullStr	Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections
title_full_unstemmed	Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections
title_sort	establishment of a striped catfish skin explant model for studying the skin response in aeromonas hydrophila infections
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/90468c487b1948d5b8432d9d4e7e26b5
work_keys_str_mv	AT rufangsiao establishmentofastripedcatfishskinexplantmodelforstudyingtheskinresponseinaeromonashydrophilainfections AT chiahsuanlin establishmentofastripedcatfishskinexplantmodelforstudyingtheskinresponseinaeromonashydrophilainfections AT lihsuanchen establishmentofastripedcatfishskinexplantmodelforstudyingtheskinresponseinaeromonashydrophilainfections AT liangchunwang establishmentofastripedcatfishskinexplantmodelforstudyingtheskinresponseinaeromonashydrophilainfections
_version_	1718378407139475456

Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections

Ejemplares similares