Accelerated oral wound healing using a pre-vascularized mucosal cell sheet

Abstract Cell sheets with pre-vascularization have recently been developed but remain relatively untested in oral wound healing. Therefore, we examined the potential utility of our newly developed pre-vascularized mucosal cell sheets in oral wound healing. Mucosal keratinocytes, fibroblasts, and end...

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Autores principales:	Jaewang Lee, Eun Hye Kim, Daiha Shin, Jong-Lyel Roh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/904ae6b467d9474ab9ab7dc45c16c530
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spelling	oai:doaj.org-article:904ae6b467d9474ab9ab7dc45c16c5302021-12-02T15:04:57ZAccelerated oral wound healing using a pre-vascularized mucosal cell sheet10.1038/s41598-017-10991-x2045-2322https://doaj.org/article/904ae6b467d9474ab9ab7dc45c16c5302017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-10991-xhttps://doaj.org/toc/2045-2322Abstract Cell sheets with pre-vascularization have recently been developed but remain relatively untested in oral wound healing. Therefore, we examined the potential utility of our newly developed pre-vascularized mucosal cell sheets in oral wound healing. Mucosal keratinocytes, fibroblasts, and endothelial progenitor cells were primarily cultured for in vitro cell expansion from mucosa and blood of Sprague-Dawley rats. Mucosal cell sheets were generated using cultured keratinocytes and plasma fibrin (K sheet) or keratinocytes and a mixture of fibrin, fibroblasts, and endothelial cells (PV sheet). Autologous sheets were transplanted on deep wounds in the buccal region of rats. The gross and histological characteristics of wound healing were compared among control wound, K sheet, and PV sheet groups. We successfully cultured and expanded keratinocytes, fibroblasts, and endothelial progenitor cells in vitro for generating mucosal cell sheets with or without pre-vascularization. In the in vivo oral wound model, compared with the control wound, the PV sheet group exhibited rapid wound closure more prominently than the K sheet group. The histological healing in the PV sheet group was similar to that in rat normal buccal mucosa without fibrosis. The pre-vascularized mucosal cell sheet exhibited in vivo efficacy in oral wound healing by promoting accelerated healing.Jaewang LeeEun Hye KimDaiha ShinJong-Lyel RohNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jaewang Lee Eun Hye Kim Daiha Shin Jong-Lyel Roh Accelerated oral wound healing using a pre-vascularized mucosal cell sheet
description	Abstract Cell sheets with pre-vascularization have recently been developed but remain relatively untested in oral wound healing. Therefore, we examined the potential utility of our newly developed pre-vascularized mucosal cell sheets in oral wound healing. Mucosal keratinocytes, fibroblasts, and endothelial progenitor cells were primarily cultured for in vitro cell expansion from mucosa and blood of Sprague-Dawley rats. Mucosal cell sheets were generated using cultured keratinocytes and plasma fibrin (K sheet) or keratinocytes and a mixture of fibrin, fibroblasts, and endothelial cells (PV sheet). Autologous sheets were transplanted on deep wounds in the buccal region of rats. The gross and histological characteristics of wound healing were compared among control wound, K sheet, and PV sheet groups. We successfully cultured and expanded keratinocytes, fibroblasts, and endothelial progenitor cells in vitro for generating mucosal cell sheets with or without pre-vascularization. In the in vivo oral wound model, compared with the control wound, the PV sheet group exhibited rapid wound closure more prominently than the K sheet group. The histological healing in the PV sheet group was similar to that in rat normal buccal mucosa without fibrosis. The pre-vascularized mucosal cell sheet exhibited in vivo efficacy in oral wound healing by promoting accelerated healing.
format	article
author	Jaewang Lee Eun Hye Kim Daiha Shin Jong-Lyel Roh
author_facet	Jaewang Lee Eun Hye Kim Daiha Shin Jong-Lyel Roh
author_sort	Jaewang Lee
title	Accelerated oral wound healing using a pre-vascularized mucosal cell sheet
title_short	Accelerated oral wound healing using a pre-vascularized mucosal cell sheet
title_full	Accelerated oral wound healing using a pre-vascularized mucosal cell sheet
title_fullStr	Accelerated oral wound healing using a pre-vascularized mucosal cell sheet
title_full_unstemmed	Accelerated oral wound healing using a pre-vascularized mucosal cell sheet
title_sort	accelerated oral wound healing using a pre-vascularized mucosal cell sheet
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/904ae6b467d9474ab9ab7dc45c16c530
work_keys_str_mv	AT jaewanglee acceleratedoralwoundhealingusingaprevascularizedmucosalcellsheet AT eunhyekim acceleratedoralwoundhealingusingaprevascularizedmucosalcellsheet AT daihashin acceleratedoralwoundhealingusingaprevascularizedmucosalcellsheet AT jonglyelroh acceleratedoralwoundhealingusingaprevascularizedmucosalcellsheet
_version_	1718388969753804800

Accelerated oral wound healing using a pre-vascularized mucosal cell sheet

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