Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination

Here, the authors present a two-photon light-sheet microscopy with an extended Bessel beam for a tunable field of view and reduced photodamage. They demonstrate long-term imaging of cellular dynamics over the whole body of medaka fish.

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Autores principales: Sota Takanezawa, Takashi Saitou, Takeshi Imamura
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/90559b29e26841108de03ff6b2703874
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spelling oai:doaj.org-article:90559b29e26841108de03ff6b27038742021-12-02T14:58:50ZWide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination10.1038/s41467-021-23249-y2041-1723https://doaj.org/article/90559b29e26841108de03ff6b27038742021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23249-yhttps://doaj.org/toc/2041-1723Here, the authors present a two-photon light-sheet microscopy with an extended Bessel beam for a tunable field of view and reduced photodamage. They demonstrate long-term imaging of cellular dynamics over the whole body of medaka fish.Sota TakanezawaTakashi SaitouTakeshi ImamuraNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Sota Takanezawa
Takashi Saitou
Takeshi Imamura
Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
description Here, the authors present a two-photon light-sheet microscopy with an extended Bessel beam for a tunable field of view and reduced photodamage. They demonstrate long-term imaging of cellular dynamics over the whole body of medaka fish.
format article
author Sota Takanezawa
Takashi Saitou
Takeshi Imamura
author_facet Sota Takanezawa
Takashi Saitou
Takeshi Imamura
author_sort Sota Takanezawa
title Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
title_short Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
title_full Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
title_fullStr Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
title_full_unstemmed Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
title_sort wide field light-sheet microscopy with lens-axicon controlled two-photon bessel beam illumination
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/90559b29e26841108de03ff6b2703874
work_keys_str_mv AT sotatakanezawa widefieldlightsheetmicroscopywithlensaxiconcontrolledtwophotonbesselbeamillumination
AT takashisaitou widefieldlightsheetmicroscopywithlensaxiconcontrolledtwophotonbesselbeamillumination
AT takeshiimamura widefieldlightsheetmicroscopywithlensaxiconcontrolledtwophotonbesselbeamillumination
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