Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
Here, the authors present a two-photon light-sheet microscopy with an extended Bessel beam for a tunable field of view and reduced photodamage. They demonstrate long-term imaging of cellular dynamics over the whole body of medaka fish.
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Nature Portfolio
2021
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oai:doaj.org-article:90559b29e26841108de03ff6b27038742021-12-02T14:58:50ZWide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination10.1038/s41467-021-23249-y2041-1723https://doaj.org/article/90559b29e26841108de03ff6b27038742021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23249-yhttps://doaj.org/toc/2041-1723Here, the authors present a two-photon light-sheet microscopy with an extended Bessel beam for a tunable field of view and reduced photodamage. They demonstrate long-term imaging of cellular dynamics over the whole body of medaka fish.Sota TakanezawaTakashi SaitouTakeshi ImamuraNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021) |
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Science Q Sota Takanezawa Takashi Saitou Takeshi Imamura Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination |
description |
Here, the authors present a two-photon light-sheet microscopy with an extended Bessel beam for a tunable field of view and reduced photodamage. They demonstrate long-term imaging of cellular dynamics over the whole body of medaka fish. |
format |
article |
author |
Sota Takanezawa Takashi Saitou Takeshi Imamura |
author_facet |
Sota Takanezawa Takashi Saitou Takeshi Imamura |
author_sort |
Sota Takanezawa |
title |
Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination |
title_short |
Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination |
title_full |
Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination |
title_fullStr |
Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination |
title_full_unstemmed |
Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination |
title_sort |
wide field light-sheet microscopy with lens-axicon controlled two-photon bessel beam illumination |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/90559b29e26841108de03ff6b2703874 |
work_keys_str_mv |
AT sotatakanezawa widefieldlightsheetmicroscopywithlensaxiconcontrolledtwophotonbesselbeamillumination AT takashisaitou widefieldlightsheetmicroscopywithlensaxiconcontrolledtwophotonbesselbeamillumination AT takeshiimamura widefieldlightsheetmicroscopywithlensaxiconcontrolledtwophotonbesselbeamillumination |
_version_ |
1718389279236816896 |