DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization

Fibromyalgia (FM) has been explained as a result of gene-environment interactions. The present study aims to verify DNA methylation differences in eleven candidate genome regions previously associated to FM, evaluating DNA methylation patterns as potential disease biomarkers. DNA methylation was ana...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Maria Carla Gerra, Davide Carnevali, Paolo Ossola, Alberto González-Villar, Inge Søkilde Pedersen, Yolanda Triñanes, Claudia Donnini, Matteo Manfredini, Lars Arendt-Nielsen, Maria Teresa Carrillo-de-la-Peña
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
R
Acceso en línea:https://doaj.org/article/90687427e78d4877915ee3849e60265b
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:90687427e78d4877915ee3849e60265b
record_format dspace
spelling oai:doaj.org-article:90687427e78d4877915ee3849e60265b2021-11-11T17:37:23ZDNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization10.3390/jcm102149922077-0383https://doaj.org/article/90687427e78d4877915ee3849e60265b2021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/4992https://doaj.org/toc/2077-0383Fibromyalgia (FM) has been explained as a result of gene-environment interactions. The present study aims to verify DNA methylation differences in eleven candidate genome regions previously associated to FM, evaluating DNA methylation patterns as potential disease biomarkers. DNA methylation was analyzed through bisulfite sequencing, comparing 42 FM women and their 42 healthy sisters. The associations between the level of methylation in these regions were further explored through a network analysis. Lastly, a logistic regression model investigated the regions potentially associated with FM, when controlling for sociodemographic variables and depressive symptoms. The analysis highlighted significant differences in the <i>GCSAML</i> region methylation between patients and controls. Moreover, seventeen single CpGs, belonging to other genes, were significantly different, however, only one cytosine related to <i>GCSAML</i> survived the correction for multiple comparisons. The network structure of methylation sites was different for each group; <i>GRM2</i> methylation represented a central node only for FM patients. Logistic regression revealed that depressive symptoms and DNA methylation in the <i>GRM2</i> region were significantly associated with FM risk. Our study encourages better exploration of <i>GCSAML</i> and <i>GRM2</i> functions and their possible role in FM affecting immune, inflammatory response, and central sensitization of pain.Maria Carla GerraDavide CarnevaliPaolo OssolaAlberto González-VillarInge Søkilde PedersenYolanda TriñanesClaudia DonniniMatteo ManfrediniLars Arendt-NielsenMaria Teresa Carrillo-de-la-PeñaMDPI AGarticlefibromyalgiaepigeneticsbloodbiomarkersDNA methylationdepressionMedicineRENJournal of Clinical Medicine, Vol 10, Iss 4992, p 4992 (2021)
institution DOAJ
collection DOAJ
language EN
topic fibromyalgia
epigenetics
blood
biomarkers
DNA methylation
depression
Medicine
R
spellingShingle fibromyalgia
epigenetics
blood
biomarkers
DNA methylation
depression
Medicine
R
Maria Carla Gerra
Davide Carnevali
Paolo Ossola
Alberto González-Villar
Inge Søkilde Pedersen
Yolanda Triñanes
Claudia Donnini
Matteo Manfredini
Lars Arendt-Nielsen
Maria Teresa Carrillo-de-la-Peña
DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization
description Fibromyalgia (FM) has been explained as a result of gene-environment interactions. The present study aims to verify DNA methylation differences in eleven candidate genome regions previously associated to FM, evaluating DNA methylation patterns as potential disease biomarkers. DNA methylation was analyzed through bisulfite sequencing, comparing 42 FM women and their 42 healthy sisters. The associations between the level of methylation in these regions were further explored through a network analysis. Lastly, a logistic regression model investigated the regions potentially associated with FM, when controlling for sociodemographic variables and depressive symptoms. The analysis highlighted significant differences in the <i>GCSAML</i> region methylation between patients and controls. Moreover, seventeen single CpGs, belonging to other genes, were significantly different, however, only one cytosine related to <i>GCSAML</i> survived the correction for multiple comparisons. The network structure of methylation sites was different for each group; <i>GRM2</i> methylation represented a central node only for FM patients. Logistic regression revealed that depressive symptoms and DNA methylation in the <i>GRM2</i> region were significantly associated with FM risk. Our study encourages better exploration of <i>GCSAML</i> and <i>GRM2</i> functions and their possible role in FM affecting immune, inflammatory response, and central sensitization of pain.
format article
author Maria Carla Gerra
Davide Carnevali
Paolo Ossola
Alberto González-Villar
Inge Søkilde Pedersen
Yolanda Triñanes
Claudia Donnini
Matteo Manfredini
Lars Arendt-Nielsen
Maria Teresa Carrillo-de-la-Peña
author_facet Maria Carla Gerra
Davide Carnevali
Paolo Ossola
Alberto González-Villar
Inge Søkilde Pedersen
Yolanda Triñanes
Claudia Donnini
Matteo Manfredini
Lars Arendt-Nielsen
Maria Teresa Carrillo-de-la-Peña
author_sort Maria Carla Gerra
title DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization
title_short DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization
title_full DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization
title_fullStr DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization
title_full_unstemmed DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization
title_sort dna methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/90687427e78d4877915ee3849e60265b
work_keys_str_mv AT mariacarlagerra dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
AT davidecarnevali dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
AT paoloossola dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
AT albertogonzalezvillar dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
AT ingesøkildepedersen dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
AT yolandatrinanes dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
AT claudiadonnini dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
AT matteomanfredini dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
AT larsarendtnielsen dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
AT mariateresacarrillodelapena dnamethylationchangesinfibromyalgiasuggesttheroleoftheimmuneinflammatoryresponseandcentralsensitization
_version_ 1718432076329385984