The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago

Abstract Caffeine is the most consumed alkaloid stimulant in the world. It is synthesized through the activity of three known N-methyltransferase proteins. Here we are reporting on the 422-Mb chromosome-level assembly of the Coffea humblotiana genome, a wild and endangered, naturally caffeine-free,...

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Autores principales: Nathalie Raharimalala, Stephane Rombauts, Andrew McCarthy, Andréa Garavito, Simon Orozco-Arias, Laurence Bellanger, Alexa Yadira Morales-Correa, Solène Froger, Stéphane Michaux, Victoria Berry, Sylviane Metairon, Coralie Fournier, Maud Lepelley, Lukas Mueller, Emmanuel Couturon, Perla Hamon, Jean-Jacques Rakotomalala, Patrick Descombes, Romain Guyot, Dominique Crouzillat
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:906bccab85c946ceb18d62f60ba4d0fe2021-12-02T14:27:59ZThe absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago10.1038/s41598-021-87419-02045-2322https://doaj.org/article/906bccab85c946ceb18d62f60ba4d0fe2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87419-0https://doaj.org/toc/2045-2322Abstract Caffeine is the most consumed alkaloid stimulant in the world. It is synthesized through the activity of three known N-methyltransferase proteins. Here we are reporting on the 422-Mb chromosome-level assembly of the Coffea humblotiana genome, a wild and endangered, naturally caffeine-free, species from the Comoro archipelago. We predicted 32,874 genes and anchored 88.7% of the sequence onto the 11 chromosomes. Comparative analyses with the African Robusta coffee genome (C. canephora) revealed an extensive genome conservation, despite an estimated 11 million years of divergence and a broad diversity of genome sizes within the Coffea genus. In this genome, the absence of caffeine is likely due to the absence of the caffeine synthase gene which converts theobromine into caffeine through an illegitimate recombination mechanism. These findings pave the way for further characterization of caffeine-free species in the Coffea genus and will guide research towards naturally-decaffeinated coffee drinks for consumers.Nathalie RaharimalalaStephane RombautsAndrew McCarthyAndréa GaravitoSimon Orozco-AriasLaurence BellangerAlexa Yadira Morales-CorreaSolène FrogerStéphane MichauxVictoria BerrySylviane MetaironCoralie FournierMaud LepelleyLukas MuellerEmmanuel CouturonPerla HamonJean-Jacques RakotomalalaPatrick DescombesRomain GuyotDominique CrouzillatNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Nathalie Raharimalala
Stephane Rombauts
Andrew McCarthy
Andréa Garavito
Simon Orozco-Arias
Laurence Bellanger
Alexa Yadira Morales-Correa
Solène Froger
Stéphane Michaux
Victoria Berry
Sylviane Metairon
Coralie Fournier
Maud Lepelley
Lukas Mueller
Emmanuel Couturon
Perla Hamon
Jean-Jacques Rakotomalala
Patrick Descombes
Romain Guyot
Dominique Crouzillat
The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago
description Abstract Caffeine is the most consumed alkaloid stimulant in the world. It is synthesized through the activity of three known N-methyltransferase proteins. Here we are reporting on the 422-Mb chromosome-level assembly of the Coffea humblotiana genome, a wild and endangered, naturally caffeine-free, species from the Comoro archipelago. We predicted 32,874 genes and anchored 88.7% of the sequence onto the 11 chromosomes. Comparative analyses with the African Robusta coffee genome (C. canephora) revealed an extensive genome conservation, despite an estimated 11 million years of divergence and a broad diversity of genome sizes within the Coffea genus. In this genome, the absence of caffeine is likely due to the absence of the caffeine synthase gene which converts theobromine into caffeine through an illegitimate recombination mechanism. These findings pave the way for further characterization of caffeine-free species in the Coffea genus and will guide research towards naturally-decaffeinated coffee drinks for consumers.
format article
author Nathalie Raharimalala
Stephane Rombauts
Andrew McCarthy
Andréa Garavito
Simon Orozco-Arias
Laurence Bellanger
Alexa Yadira Morales-Correa
Solène Froger
Stéphane Michaux
Victoria Berry
Sylviane Metairon
Coralie Fournier
Maud Lepelley
Lukas Mueller
Emmanuel Couturon
Perla Hamon
Jean-Jacques Rakotomalala
Patrick Descombes
Romain Guyot
Dominique Crouzillat
author_facet Nathalie Raharimalala
Stephane Rombauts
Andrew McCarthy
Andréa Garavito
Simon Orozco-Arias
Laurence Bellanger
Alexa Yadira Morales-Correa
Solène Froger
Stéphane Michaux
Victoria Berry
Sylviane Metairon
Coralie Fournier
Maud Lepelley
Lukas Mueller
Emmanuel Couturon
Perla Hamon
Jean-Jacques Rakotomalala
Patrick Descombes
Romain Guyot
Dominique Crouzillat
author_sort Nathalie Raharimalala
title The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago
title_short The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago
title_full The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago
title_fullStr The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago
title_full_unstemmed The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago
title_sort absence of the caffeine synthase gene is involved in the naturally decaffeinated status of coffea humblotiana, a wild species from comoro archipelago
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/906bccab85c946ceb18d62f60ba4d0fe
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