Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats

Abstract Multiple sclerosis (MS) is an autoimmune disease that usually occurs during the reproductive years in both sexes. Many male patients with MS show lower blood testosterone levels, which was also observed in male rats during experimental autoimmune encephalomyelitis (EAE), an animal model of...

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Autores principales:	Ana Milosevic, Ivana Bjelobaba, Iva D. Bozic, Irena Lavrnja, Danijela Savic, Katarina Tesovic, Marija Jakovljevic, Stanko S. Stojilkovic, Marija M. Janjic
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/906ed3a3f36448469a6ecd1d6ea50371
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spelling	oai:doaj.org-article:906ed3a3f36448469a6ecd1d6ea503712021-12-02T17:39:31ZTesticular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats10.1038/s41598-021-88305-52045-2322https://doaj.org/article/906ed3a3f36448469a6ecd1d6ea503712021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88305-5https://doaj.org/toc/2045-2322Abstract Multiple sclerosis (MS) is an autoimmune disease that usually occurs during the reproductive years in both sexes. Many male patients with MS show lower blood testosterone levels, which was also observed in male rats during experimental autoimmune encephalomyelitis (EAE), an animal model of MS. To better understand the causes of decreased testosterone production during EAE, we investigated the expression status of genes and proteins associated with steroidogenesis in the testes. No changes in the number of interstitial cells were observed in EAE animals, but the expression of the insulin-like 3 gene was reduced at the peak of the disease, implying that the Leydig cell functional capacity was affected. Consistent with this finding, the expression of most steroidogenic enzyme genes and proteins was reduced during EAE, including StAR, CYP11A1, CYP17A1 and HSD3B. No signs of testicular inflammation were observed. Recovery of steroidogenesis was observed after injection of hCG, the placental gonadotropin, or buserelin acetate, a gonadotropin-releasing hormone analogue, at the peak of EAE. Together, our results are consistent with the hypothesis that impaired testicular steroidogenesis originates upstream of the testes and that low serum LH is the main cause of decreased testosterone levels during EAE.Ana MilosevicIvana BjelobabaIva D. BozicIrena LavrnjaDanijela SavicKatarina TesovicMarija JakovljevicStanko S. StojilkovicMarija M. JanjicNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Milosevic Ivana Bjelobaba Iva D. Bozic Irena Lavrnja Danijela Savic Katarina Tesovic Marija Jakovljevic Stanko S. Stojilkovic Marija M. Janjic Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats
description	Abstract Multiple sclerosis (MS) is an autoimmune disease that usually occurs during the reproductive years in both sexes. Many male patients with MS show lower blood testosterone levels, which was also observed in male rats during experimental autoimmune encephalomyelitis (EAE), an animal model of MS. To better understand the causes of decreased testosterone production during EAE, we investigated the expression status of genes and proteins associated with steroidogenesis in the testes. No changes in the number of interstitial cells were observed in EAE animals, but the expression of the insulin-like 3 gene was reduced at the peak of the disease, implying that the Leydig cell functional capacity was affected. Consistent with this finding, the expression of most steroidogenic enzyme genes and proteins was reduced during EAE, including StAR, CYP11A1, CYP17A1 and HSD3B. No signs of testicular inflammation were observed. Recovery of steroidogenesis was observed after injection of hCG, the placental gonadotropin, or buserelin acetate, a gonadotropin-releasing hormone analogue, at the peak of EAE. Together, our results are consistent with the hypothesis that impaired testicular steroidogenesis originates upstream of the testes and that low serum LH is the main cause of decreased testosterone levels during EAE.
format	article
author	Ana Milosevic Ivana Bjelobaba Iva D. Bozic Irena Lavrnja Danijela Savic Katarina Tesovic Marija Jakovljevic Stanko S. Stojilkovic Marija M. Janjic
author_facet	Ana Milosevic Ivana Bjelobaba Iva D. Bozic Irena Lavrnja Danijela Savic Katarina Tesovic Marija Jakovljevic Stanko S. Stojilkovic Marija M. Janjic
author_sort	Ana Milosevic
title	Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats
title_short	Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats
title_full	Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats
title_fullStr	Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats
title_full_unstemmed	Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats
title_sort	testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/906ed3a3f36448469a6ecd1d6ea50371
work_keys_str_mv	AT anamilosevic testicularsteroidogenesisissuppressedduringexperimentalautoimmuneencephalomyelitisinrats AT ivanabjelobaba testicularsteroidogenesisissuppressedduringexperimentalautoimmuneencephalomyelitisinrats AT ivadbozic testicularsteroidogenesisissuppressedduringexperimentalautoimmuneencephalomyelitisinrats AT irenalavrnja testicularsteroidogenesisissuppressedduringexperimentalautoimmuneencephalomyelitisinrats AT danijelasavic testicularsteroidogenesisissuppressedduringexperimentalautoimmuneencephalomyelitisinrats AT katarinatesovic testicularsteroidogenesisissuppressedduringexperimentalautoimmuneencephalomyelitisinrats AT marijajakovljevic testicularsteroidogenesisissuppressedduringexperimentalautoimmuneencephalomyelitisinrats AT stankosstojilkovic testicularsteroidogenesisissuppressedduringexperimentalautoimmuneencephalomyelitisinrats AT marijamjanjic testicularsteroidogenesisissuppressedduringexperimentalautoimmuneencephalomyelitisinrats
_version_	1718379835529625600

Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats

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