Biodegradation of Brown 706 Dye by Bacterial Strain <i>Pseudomonas aeruginosa</i>

Dyes are the most challenging pollutants for the aquatic environment that are not only toxic, but also interfering photosynthesis as light penetration into deep water is changed. A number of methods are used for the water reclamation, however, among them biological methods are preferably used due to...

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Autores principales:	Asad Ullah Khan, Mujaddad Ur Rehman, Muhammad Zahoor, Abdul Bari Shah, Ivar Zekker
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biodegradation Brown 706 dye <i>Pseudomonas aeruginosa</i> textile wastewater Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
Acceso en línea:	https://doaj.org/article/90707227bb41489e8692b0deb9ce66ee
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spelling	oai:doaj.org-article:90707227bb41489e8692b0deb9ce66ee2021-11-11T19:52:40ZBiodegradation of Brown 706 Dye by Bacterial Strain <i>Pseudomonas aeruginosa</i>10.3390/w132129592073-4441https://doaj.org/article/90707227bb41489e8692b0deb9ce66ee2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4441/13/21/2959https://doaj.org/toc/2073-4441Dyes are the most challenging pollutants for the aquatic environment that are not only toxic, but also interfering photosynthesis as light penetration into deep water is changed. A number of methods are used for the water reclamation, however, among them biological methods are preferably used due to their compatibility with nature. In the present research, 15 different bacterial strains were used to decolorize Brown 706 dye. Among the bacterial strains, <i>Pseudomonas aeruginosa</i> showed maximum decolorization activity; hence in the subsequent experiments <i>Pseudomonas aeruginosa</i> was used. First the decolorization activities were carried out under different physicochemical conditions to obtain the optimum decolorization benefits of the selected microorganism. The optimum conditions established were 37°C, pH of 7 and operation cycle time 72 h. In the subsequent experiment all optimum conditions were combined in a single experiment where 73.91% of decolorization efficiency was achieved. For the evaluation of metabolites formed after decolorization/degradation the aliquots containing bacteria were homogenized, filtered and then subjected to extraction. The extracted metabolites were then subjected to the silica gel column isolation. UV–Vis, FTIR, and NMR techniques were used to elucidate structures of the metabolites. Out of the collected metabolites only P-xylene was identified, which has been formed by cleavage of azo linkage by azo reductase enzyme of bacteria following the deamination and methylation of nitro substituted benzene ring.Asad Ullah KhanMujaddad Ur RehmanMuhammad ZahoorAbdul Bari ShahIvar ZekkerMDPI AGarticlebiodegradationBrown 706 dye<i>Pseudomonas aeruginosa</i>textile wastewaterHydraulic engineeringTC1-978Water supply for domestic and industrial purposesTD201-500ENWater, Vol 13, Iss 2959, p 2959 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biodegradation Brown 706 dye <i>Pseudomonas aeruginosa</i> textile wastewater Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
spellingShingle	biodegradation Brown 706 dye <i>Pseudomonas aeruginosa</i> textile wastewater Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 Asad Ullah Khan Mujaddad Ur Rehman Muhammad Zahoor Abdul Bari Shah Ivar Zekker Biodegradation of Brown 706 Dye by Bacterial Strain <i>Pseudomonas aeruginosa</i>
description	Dyes are the most challenging pollutants for the aquatic environment that are not only toxic, but also interfering photosynthesis as light penetration into deep water is changed. A number of methods are used for the water reclamation, however, among them biological methods are preferably used due to their compatibility with nature. In the present research, 15 different bacterial strains were used to decolorize Brown 706 dye. Among the bacterial strains, <i>Pseudomonas aeruginosa</i> showed maximum decolorization activity; hence in the subsequent experiments <i>Pseudomonas aeruginosa</i> was used. First the decolorization activities were carried out under different physicochemical conditions to obtain the optimum decolorization benefits of the selected microorganism. The optimum conditions established were 37°C, pH of 7 and operation cycle time 72 h. In the subsequent experiment all optimum conditions were combined in a single experiment where 73.91% of decolorization efficiency was achieved. For the evaluation of metabolites formed after decolorization/degradation the aliquots containing bacteria were homogenized, filtered and then subjected to extraction. The extracted metabolites were then subjected to the silica gel column isolation. UV–Vis, FTIR, and NMR techniques were used to elucidate structures of the metabolites. Out of the collected metabolites only P-xylene was identified, which has been formed by cleavage of azo linkage by azo reductase enzyme of bacteria following the deamination and methylation of nitro substituted benzene ring.
format	article
author	Asad Ullah Khan Mujaddad Ur Rehman Muhammad Zahoor Abdul Bari Shah Ivar Zekker
author_facet	Asad Ullah Khan Mujaddad Ur Rehman Muhammad Zahoor Abdul Bari Shah Ivar Zekker
author_sort	Asad Ullah Khan
title	Biodegradation of Brown 706 Dye by Bacterial Strain <i>Pseudomonas aeruginosa</i>
title_short	Biodegradation of Brown 706 Dye by Bacterial Strain <i>Pseudomonas aeruginosa</i>
title_full	Biodegradation of Brown 706 Dye by Bacterial Strain <i>Pseudomonas aeruginosa</i>
title_fullStr	Biodegradation of Brown 706 Dye by Bacterial Strain <i>Pseudomonas aeruginosa</i>
title_full_unstemmed	Biodegradation of Brown 706 Dye by Bacterial Strain <i>Pseudomonas aeruginosa</i>
title_sort	biodegradation of brown 706 dye by bacterial strain <i>pseudomonas aeruginosa</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/90707227bb41489e8692b0deb9ce66ee
work_keys_str_mv	AT asadullahkhan biodegradationofbrown706dyebybacterialstrainipseudomonasaeruginosai AT mujaddadurrehman biodegradationofbrown706dyebybacterialstrainipseudomonasaeruginosai AT muhammadzahoor biodegradationofbrown706dyebybacterialstrainipseudomonasaeruginosai AT abdulbarishah biodegradationofbrown706dyebybacterialstrainipseudomonasaeruginosai AT ivarzekker biodegradationofbrown706dyebybacterialstrainipseudomonasaeruginosai
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Biodegradation of Brown 706 Dye by Bacterial Strain <i>Pseudomonas aeruginosa</i>

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