NESH regulates dendritic spine morphology and synapse formation.

<h4>Background</h4>Dendritic spines are small membranous protrusions on the neuronal dendrites that receive synaptic input from axon terminals. Despite their importance for integrating the enormous information flow in the brain, the molecular mechanisms regulating spine morphogenesis are...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jeomil Bae, Bong Hwan Sung, In Ha Cho, Seon-Myung Kim, Woo Keun Song
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/90756f70787c402da9bd1dade2b133db
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:90756f70787c402da9bd1dade2b133db
record_format	dspace
spelling	oai:doaj.org-article:90756f70787c402da9bd1dade2b133db2021-11-18T07:23:26ZNESH regulates dendritic spine morphology and synapse formation.1932-620310.1371/journal.pone.0034677https://doaj.org/article/90756f70787c402da9bd1dade2b133db2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22485184/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Dendritic spines are small membranous protrusions on the neuronal dendrites that receive synaptic input from axon terminals. Despite their importance for integrating the enormous information flow in the brain, the molecular mechanisms regulating spine morphogenesis are not well understood. NESH/Abi-3 is a member of the Abl interactor (Abi) protein family, and its overexpression is known to reduce cell motility and tumor metastasis. NESH is prominently expressed in the brain, but its function there remains unknown.<h4>Methodology/principal findings</h4>NESH was strongly expressed in the hippocampus and moderately expressed in the cerebral cortex, cerebellum and striatum, where it co-localized with the postsynaptic proteins PSD95, SPIN90 and F-actin in dendritic spines. Overexpression of NESH reduced numbers of mushroom-type spines and synapse density but increased thin, filopodia-like spines and had no effect on spine density. siRNA knockdown of NESH also reduced mushroom spine numbers and inhibited synapse formation but it increased spine density. The N-terminal region of NESH co-sedimented with filamentous actin (F-actin), which is an essential component of dendritic spines, suggesting this interaction is important for the maturation of dendritic spines.<h4>Conclusions/significance</h4>NESH is a novel F-actin binding protein that likely plays important roles in the regulation of dendritic spine morphogenesis and synapse formation.Jeomil BaeBong Hwan SungIn Ha ChoSeon-Myung KimWoo Keun SongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34677 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeomil Bae Bong Hwan Sung In Ha Cho Seon-Myung Kim Woo Keun Song NESH regulates dendritic spine morphology and synapse formation.
description	<h4>Background</h4>Dendritic spines are small membranous protrusions on the neuronal dendrites that receive synaptic input from axon terminals. Despite their importance for integrating the enormous information flow in the brain, the molecular mechanisms regulating spine morphogenesis are not well understood. NESH/Abi-3 is a member of the Abl interactor (Abi) protein family, and its overexpression is known to reduce cell motility and tumor metastasis. NESH is prominently expressed in the brain, but its function there remains unknown.<h4>Methodology/principal findings</h4>NESH was strongly expressed in the hippocampus and moderately expressed in the cerebral cortex, cerebellum and striatum, where it co-localized with the postsynaptic proteins PSD95, SPIN90 and F-actin in dendritic spines. Overexpression of NESH reduced numbers of mushroom-type spines and synapse density but increased thin, filopodia-like spines and had no effect on spine density. siRNA knockdown of NESH also reduced mushroom spine numbers and inhibited synapse formation but it increased spine density. The N-terminal region of NESH co-sedimented with filamentous actin (F-actin), which is an essential component of dendritic spines, suggesting this interaction is important for the maturation of dendritic spines.<h4>Conclusions/significance</h4>NESH is a novel F-actin binding protein that likely plays important roles in the regulation of dendritic spine morphogenesis and synapse formation.
format	article
author	Jeomil Bae Bong Hwan Sung In Ha Cho Seon-Myung Kim Woo Keun Song
author_facet	Jeomil Bae Bong Hwan Sung In Ha Cho Seon-Myung Kim Woo Keun Song
author_sort	Jeomil Bae
title	NESH regulates dendritic spine morphology and synapse formation.
title_short	NESH regulates dendritic spine morphology and synapse formation.
title_full	NESH regulates dendritic spine morphology and synapse formation.
title_fullStr	NESH regulates dendritic spine morphology and synapse formation.
title_full_unstemmed	NESH regulates dendritic spine morphology and synapse formation.
title_sort	nesh regulates dendritic spine morphology and synapse formation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/90756f70787c402da9bd1dade2b133db
work_keys_str_mv	AT jeomilbae neshregulatesdendriticspinemorphologyandsynapseformation AT bonghwansung neshregulatesdendriticspinemorphologyandsynapseformation AT inhacho neshregulatesdendriticspinemorphologyandsynapseformation AT seonmyungkim neshregulatesdendriticspinemorphologyandsynapseformation AT wookeunsong neshregulatesdendriticspinemorphologyandsynapseformation
_version_	1718423546964738048

NESH regulates dendritic spine morphology and synapse formation.

Ejemplares similares