$Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging$
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Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

Abstract Cell refractive index, an intrinsic optical parameter, is closely correlated with the intracellular mass and concentration. By combining optical phase-shifting interferometry (PSI) and atomic force microscope (AFM) imaging, we constructed a label free, non-invasive and quantitative refracti...

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Autores principales:	Qinnan Zhang, Liyun Zhong, Ping Tang, Yingjie Yuan, Shengde Liu, Jindong Tian, Xiaoxu Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9076c89ffbff4fce880c580fedaa510c
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spelling	oai:doaj.org-article:9076c89ffbff4fce880c580fedaa510c2021-12-02T16:08:20ZQuantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging10.1038/s41598-017-02797-82045-2322https://doaj.org/article/9076c89ffbff4fce880c580fedaa510c2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02797-8https://doaj.org/toc/2045-2322Abstract Cell refractive index, an intrinsic optical parameter, is closely correlated with the intracellular mass and concentration. By combining optical phase-shifting interferometry (PSI) and atomic force microscope (AFM) imaging, we constructed a label free, non-invasive and quantitative refractive index of single cell measurement system, in which the accurate phase map of single cell was retrieved with PSI technique and the cell morphology with nanoscale resolution was achieved with AFM imaging. Based on the proposed AFM/PSI system, we achieved quantitative refractive index distributions of single red blood cell and Jurkat cell, respectively. Further, the quantitative change of refractive index distribution during Daunorubicin (DNR)-induced Jurkat cell apoptosis was presented, and then the content changes of intracellular biochemical components were achieved. Importantly, these results were consistent with Raman spectral analysis, indicating that the proposed PSI/AFM based refractive index system is likely to become a useful tool for intracellular biochemical components analysis measurement, and this will facilitate its application for revealing cell structure and pathological state from a new perspective.Qinnan ZhangLiyun ZhongPing TangYingjie YuanShengde LiuJindong TianXiaoxu LuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qinnan Zhang Liyun Zhong Ping Tang Yingjie Yuan Shengde Liu Jindong Tian Xiaoxu Lu Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging
description	Abstract Cell refractive index, an intrinsic optical parameter, is closely correlated with the intracellular mass and concentration. By combining optical phase-shifting interferometry (PSI) and atomic force microscope (AFM) imaging, we constructed a label free, non-invasive and quantitative refractive index of single cell measurement system, in which the accurate phase map of single cell was retrieved with PSI technique and the cell morphology with nanoscale resolution was achieved with AFM imaging. Based on the proposed AFM/PSI system, we achieved quantitative refractive index distributions of single red blood cell and Jurkat cell, respectively. Further, the quantitative change of refractive index distribution during Daunorubicin (DNR)-induced Jurkat cell apoptosis was presented, and then the content changes of intracellular biochemical components were achieved. Importantly, these results were consistent with Raman spectral analysis, indicating that the proposed PSI/AFM based refractive index system is likely to become a useful tool for intracellular biochemical components analysis measurement, and this will facilitate its application for revealing cell structure and pathological state from a new perspective.
format	article
author	Qinnan Zhang Liyun Zhong Ping Tang Yingjie Yuan Shengde Liu Jindong Tian Xiaoxu Lu
author_facet	Qinnan Zhang Liyun Zhong Ping Tang Yingjie Yuan Shengde Liu Jindong Tian Xiaoxu Lu
author_sort	Qinnan Zhang
title	Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging
title_short	Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging
title_full	Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging
title_fullStr	Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging
title_full_unstemmed	Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging
title_sort	quantitative refractive index distribution of single cell by combining phase-shifting interferometry and afm imaging
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9076c89ffbff4fce880c580fedaa510c
work_keys_str_mv	AT qinnanzhang quantitativerefractiveindexdistributionofsinglecellbycombiningphaseshiftinginterferometryandafmimaging AT liyunzhong quantitativerefractiveindexdistributionofsinglecellbycombiningphaseshiftinginterferometryandafmimaging AT pingtang quantitativerefractiveindexdistributionofsinglecellbycombiningphaseshiftinginterferometryandafmimaging AT yingjieyuan quantitativerefractiveindexdistributionofsinglecellbycombiningphaseshiftinginterferometryandafmimaging AT shengdeliu quantitativerefractiveindexdistributionofsinglecellbycombiningphaseshiftinginterferometryandafmimaging AT jindongtian quantitativerefractiveindexdistributionofsinglecellbycombiningphaseshiftinginterferometryandafmimaging AT xiaoxulu quantitativerefractiveindexdistributionofsinglecellbycombiningphaseshiftinginterferometryandafmimaging
_version_	1718384544129744896

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

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