Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid

Chang-Gu Keum1*, Young-Wook Noh1*, Jong-Suep Baek1, Ji-Ho Lim1, Chan-Ju Hwang1, Young-Guk Na1, Sang-Chul Shin2, Cheong-Weon Cho11College of Pharmacy and Institute of Drug Research and Development, Chungnam National University, Gungdong, Yuseonggu, Daejeon, South Korea; 2College of Pharmacy, Chonnam...

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Autores principales:	Keum CG, Noh YW, Baek JS, Lim JH, Hwang CJ, Na YG, Shin SC, Cho CW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/907bb828f6ab4cb0819f6ab1c4aa388e
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spelling	oai:doaj.org-article:907bb828f6ab4cb0819f6ab1c4aa388e2021-12-02T02:10:28ZPractical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid1176-91141178-2013https://doaj.org/article/907bb828f6ab4cb0819f6ab1c4aa388e2011-10-01T00:00:00Zhttp://www.dovepress.com/practical-preparation-procedures-for-docetaxel-loaded-nanoparticles-us-a8418https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chang-Gu Keum1, Young-Wook Noh1, Jong-Suep Baek1, Ji-Ho Lim1, Chan-Ju Hwang1, Young-Guk Na1, Sang-Chul Shin2, Cheong-Weon Cho11College of Pharmacy and Institute of Drug Research and Development, Chungnam National University, Gungdong, Yuseonggu, Daejeon, South Korea; 2College of Pharmacy, Chonnam National University, Yongbongdong, Buggu, Gwangju, South Korea *These authors contributed equally to this work Background: Nanoparticles fabricated from the biodegradable and biocompatible polymer, polylactic-co-glycolic acid (PLGA), are the most intensively investigated polymers for drug delivery systems. The objective of this study was to explore fully the development of a PLGA nanoparticle drug delivery system for alternative preparation of a commercial formulation. In our nanoparticle fabrication, our purpose was to compare various preparation parameters. Methods: Docetaxel-loaded PLGA nanoparticles were prepared by a single emulsion technique and solvent evaporation. The nanoparticles were characterized by various techniques, including scanning electron microscopy for surface morphology, dynamic light scattering for size and zeta potential, x-ray photoelectron spectroscopy for surface chemistry, and high-performance liquid chromatography for in vitro drug release kinetics. To obtain a smaller particle, 0.2% polyvinyl alcohol, 0.03% D-&alpha;-tocopheryl polyethylene glycol 1000 succinate (TPGS), 2% Poloxamer 188, a five-minute sonication time, 130 W sonication power, evaporation with magnetic stirring, and centrifugation at 8000 rpm were selected. To increase encapsulation efficiency in the nanoparticles, certain factors were varied, ie, 2&ndash;5 minutes of sonication time, 70&ndash;130 W sonication power, and 5&ndash;25 mg drug loading. Results: A five-minute sonication time, 130 W sonication power, and a 10 mg drug loading amount were selected. Under these conditions, the nanoparticles reached over 90% encapsulation efficiency. Release kinetics showed that 20.83%, 40.07%, and 51.5% of the docetaxel was released in 28 days from nanoparticles containing Poloxamer 188, TPGS, or polyvinyl alcohol, respectively. TPGS and Poloxamer 188 had slower release kinetics than polyvinyl alcohol. It was predicted that there was residual drug remaining on the surface from x-ray photoelectron spectroscopy. Conclusion: Our research shows that the choice of surfactant is important for controlled release of docetaxel. Keywords: docetaxel, nanoparticles, poly (lactic-co-glycolic acid), formulation variables, sonication, encapsulation efficiencyKeum CGNoh YWBaek JSLim JHHwang CJNa YGShin SCCho CWDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2225-2234 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Keum CG Noh YW Baek JS Lim JH Hwang CJ Na YG Shin SC Cho CW Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid
description	Chang-Gu Keum1, Young-Wook Noh1, Jong-Suep Baek1, Ji-Ho Lim1, Chan-Ju Hwang1, Young-Guk Na1, Sang-Chul Shin2, Cheong-Weon Cho11College of Pharmacy and Institute of Drug Research and Development, Chungnam National University, Gungdong, Yuseonggu, Daejeon, South Korea; 2College of Pharmacy, Chonnam National University, Yongbongdong, Buggu, Gwangju, South Korea *These authors contributed equally to this work Background: Nanoparticles fabricated from the biodegradable and biocompatible polymer, polylactic-co-glycolic acid (PLGA), are the most intensively investigated polymers for drug delivery systems. The objective of this study was to explore fully the development of a PLGA nanoparticle drug delivery system for alternative preparation of a commercial formulation. In our nanoparticle fabrication, our purpose was to compare various preparation parameters. Methods: Docetaxel-loaded PLGA nanoparticles were prepared by a single emulsion technique and solvent evaporation. The nanoparticles were characterized by various techniques, including scanning electron microscopy for surface morphology, dynamic light scattering for size and zeta potential, x-ray photoelectron spectroscopy for surface chemistry, and high-performance liquid chromatography for in vitro drug release kinetics. To obtain a smaller particle, 0.2% polyvinyl alcohol, 0.03% D-&alpha;-tocopheryl polyethylene glycol 1000 succinate (TPGS), 2% Poloxamer 188, a five-minute sonication time, 130 W sonication power, evaporation with magnetic stirring, and centrifugation at 8000 rpm were selected. To increase encapsulation efficiency in the nanoparticles, certain factors were varied, ie, 2&ndash;5 minutes of sonication time, 70&ndash;130 W sonication power, and 5&ndash;25 mg drug loading. Results: A five-minute sonication time, 130 W sonication power, and a 10 mg drug loading amount were selected. Under these conditions, the nanoparticles reached over 90% encapsulation efficiency. Release kinetics showed that 20.83%, 40.07%, and 51.5% of the docetaxel was released in 28 days from nanoparticles containing Poloxamer 188, TPGS, or polyvinyl alcohol, respectively. TPGS and Poloxamer 188 had slower release kinetics than polyvinyl alcohol. It was predicted that there was residual drug remaining on the surface from x-ray photoelectron spectroscopy. Conclusion: Our research shows that the choice of surfactant is important for controlled release of docetaxel. Keywords: docetaxel, nanoparticles, poly (lactic-co-glycolic acid), formulation variables, sonication, encapsulation efficiency
format	article
author	Keum CG Noh YW Baek JS Lim JH Hwang CJ Na YG Shin SC Cho CW
author_facet	Keum CG Noh YW Baek JS Lim JH Hwang CJ Na YG Shin SC Cho CW
author_sort	Keum CG
title	Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid
title_short	Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid
title_full	Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid
title_fullStr	Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid
title_full_unstemmed	Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid
title_sort	practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/907bb828f6ab4cb0819f6ab1c4aa388e
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Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid

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