Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand.

Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand-Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% i...

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Autores principales:	Kingkan Saokham, Nuannapa Hemniam, Sukanya Roekwan, Sirikan Hunsawattanakul, Jutathip Thawinampan, Wanwisa Siriwan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9085f53eb2504a39b3aa645db2c10dae
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spelling	oai:doaj.org-article:9085f53eb2504a39b3aa645db2c10dae2021-12-02T20:13:45ZSurvey and molecular detection of Sri Lankan cassava mosaic virus in Thailand.1932-620310.1371/journal.pone.0252846https://doaj.org/article/9085f53eb2504a39b3aa645db2c10dae2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252846https://doaj.org/toc/1932-6203Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand-Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease incidence of CMD was highest 43.08% in Sakaeo, followed by 26.78% in Prachinburi, 7% in Burium, 2.58% in Surin, and 1.25% in Sisaket provinces. Disease severity of CMD symptoms was mild chlorosis to moderate mosaic (2-3). The greatest disease severity was recorded in Prachinburi and Sakaeo provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Cassava cultivars CMR-89 and Huai Bong 80 were susceptible to CMD. In 95% of cases, the infection was transmitted by whiteflies (Bemisia tabaci), which were abundant in Sakaeo, Buriram, and Prachinburi but were sparse in Surin; their densities were highest in May and June 2019. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whiteflies in Thailand revealed that it was similar to the mtCO1 gene of Asia II 1 whitefly. Furthermore, the AV1 gene of SLCMV-which encodes the capsid protein-showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.Kingkan SaokhamNuannapa HemniamSukanya RoekwanSirikan HunsawattanakulJutathip ThawinampanWanwisa SiriwanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0252846 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kingkan Saokham Nuannapa Hemniam Sukanya Roekwan Sirikan Hunsawattanakul Jutathip Thawinampan Wanwisa Siriwan Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand.
description	Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand-Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease incidence of CMD was highest 43.08% in Sakaeo, followed by 26.78% in Prachinburi, 7% in Burium, 2.58% in Surin, and 1.25% in Sisaket provinces. Disease severity of CMD symptoms was mild chlorosis to moderate mosaic (2-3). The greatest disease severity was recorded in Prachinburi and Sakaeo provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Cassava cultivars CMR-89 and Huai Bong 80 were susceptible to CMD. In 95% of cases, the infection was transmitted by whiteflies (Bemisia tabaci), which were abundant in Sakaeo, Buriram, and Prachinburi but were sparse in Surin; their densities were highest in May and June 2019. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whiteflies in Thailand revealed that it was similar to the mtCO1 gene of Asia II 1 whitefly. Furthermore, the AV1 gene of SLCMV-which encodes the capsid protein-showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.
format	article
author	Kingkan Saokham Nuannapa Hemniam Sukanya Roekwan Sirikan Hunsawattanakul Jutathip Thawinampan Wanwisa Siriwan
author_facet	Kingkan Saokham Nuannapa Hemniam Sukanya Roekwan Sirikan Hunsawattanakul Jutathip Thawinampan Wanwisa Siriwan
author_sort	Kingkan Saokham
title	Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand.
title_short	Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand.
title_full	Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand.
title_fullStr	Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand.
title_full_unstemmed	Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand.
title_sort	survey and molecular detection of sri lankan cassava mosaic virus in thailand.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9085f53eb2504a39b3aa645db2c10dae
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Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand.

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