Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma

Background: Autophagy is a cellular process responsible for maintaining homeostasis; a dysregulation of this process is involved in the development and progression of neoplasms. Beclin-1 is one of the major proteins linked to autophagy. However, the data regarding the association between the role of...

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Autores principales:	Ida Barca, Chiara Mignogna, Daniela Novembre, Francesco Ferragina, Maria Giulia Cristofaro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Beclin-1 maxillofacial surgery head and neck cancer oral cancer oncological surgery Medicine R
Acceso en línea:	https://doaj.org/article/908bf81b351a4dbba7c53c33fbbe7c42
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spelling	oai:doaj.org-article:908bf81b351a4dbba7c53c33fbbe7c422021-11-11T16:17:26ZImmunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma10.3390/ijerph1821111251660-46011661-7827https://doaj.org/article/908bf81b351a4dbba7c53c33fbbe7c422021-10-01T00:00:00Zhttps://www.mdpi.com/1660-4601/18/21/11125https://doaj.org/toc/1661-7827https://doaj.org/toc/1660-4601Background: Autophagy is a cellular process responsible for maintaining homeostasis; a dysregulation of this process is involved in the development and progression of neoplasms. Beclin-1 is one of the major proteins linked to autophagy. However, the data regarding the association between the role of Beclin-1 and the progression of Oral Squamous Cell Carcinoma (OSCC) are rather low. For this reason, the objective of this study is to evaluate, through immunohistochemical techniques, the prognostic role of the expression of Beclin-1 autophagy marker in patients with OSCC. Methods: This is a single-centre retrospective study that includes patients with OSCC admitted to the Maxillofacial Unit of “Magna Graecia” University between January 2019 and September 2020. All the samples obtained from surgery were treated with anti Beclin-1 antibodies and subjected to immunohistochemical methods. Results: A total of 26 samples were analysed and the following variables were evaluated for each: percentage of positive Beclin-1 expression by tumour cells, signal strength of tumour cells, and total score. The variables considered were first normalised according to the D’Agostino and Pearson test, then analysed using the Pearson linear correlation coefficient: a statistically significant correlation was found between the parameters infiltration-intensity (<i>p</i> = 0.0088), infiltration-percent (<i>p</i> = 0.0123), intensity-total score (<i>p</i> = 0.0060). Conclusions: The immunohistochemical evaluation of Beclin-1 revealed a statistically significant correlation between the intensity of the molecule’s expression and a greater degree of infiltration of the neoplasm. Beclin-1 can, therefore, be considered a valid prognostic index of disease.Ida BarcaChiara MignognaDaniela NovembreFrancesco FerraginaMaria Giulia CristofaroMDPI AGarticleBeclin-1maxillofacial surgeryhead and neck canceroral canceroncological surgeryMedicineRENInternational Journal of Environmental Research and Public Health, Vol 18, Iss 11125, p 11125 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Beclin-1 maxillofacial surgery head and neck cancer oral cancer oncological surgery Medicine R
spellingShingle	Beclin-1 maxillofacial surgery head and neck cancer oral cancer oncological surgery Medicine R Ida Barca Chiara Mignogna Daniela Novembre Francesco Ferragina Maria Giulia Cristofaro Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma
description	Background: Autophagy is a cellular process responsible for maintaining homeostasis; a dysregulation of this process is involved in the development and progression of neoplasms. Beclin-1 is one of the major proteins linked to autophagy. However, the data regarding the association between the role of Beclin-1 and the progression of Oral Squamous Cell Carcinoma (OSCC) are rather low. For this reason, the objective of this study is to evaluate, through immunohistochemical techniques, the prognostic role of the expression of Beclin-1 autophagy marker in patients with OSCC. Methods: This is a single-centre retrospective study that includes patients with OSCC admitted to the Maxillofacial Unit of “Magna Graecia” University between January 2019 and September 2020. All the samples obtained from surgery were treated with anti Beclin-1 antibodies and subjected to immunohistochemical methods. Results: A total of 26 samples were analysed and the following variables were evaluated for each: percentage of positive Beclin-1 expression by tumour cells, signal strength of tumour cells, and total score. The variables considered were first normalised according to the D’Agostino and Pearson test, then analysed using the Pearson linear correlation coefficient: a statistically significant correlation was found between the parameters infiltration-intensity (<i>p</i> = 0.0088), infiltration-percent (<i>p</i> = 0.0123), intensity-total score (<i>p</i> = 0.0060). Conclusions: The immunohistochemical evaluation of Beclin-1 revealed a statistically significant correlation between the intensity of the molecule’s expression and a greater degree of infiltration of the neoplasm. Beclin-1 can, therefore, be considered a valid prognostic index of disease.
format	article
author	Ida Barca Chiara Mignogna Daniela Novembre Francesco Ferragina Maria Giulia Cristofaro
author_facet	Ida Barca Chiara Mignogna Daniela Novembre Francesco Ferragina Maria Giulia Cristofaro
author_sort	Ida Barca
title	Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma
title_short	Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma
title_full	Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma
title_fullStr	Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma
title_full_unstemmed	Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma
title_sort	immunohistochemical analysis of the beclin-1 expression predicts the progression of oral squamous cell carcinoma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/908bf81b351a4dbba7c53c33fbbe7c42
work_keys_str_mv	AT idabarca immunohistochemicalanalysisofthebeclin1expressionpredictstheprogressionoforalsquamouscellcarcinoma AT chiaramignogna immunohistochemicalanalysisofthebeclin1expressionpredictstheprogressionoforalsquamouscellcarcinoma AT danielanovembre immunohistochemicalanalysisofthebeclin1expressionpredictstheprogressionoforalsquamouscellcarcinoma AT francescoferragina immunohistochemicalanalysisofthebeclin1expressionpredictstheprogressionoforalsquamouscellcarcinoma AT mariagiuliacristofaro immunohistochemicalanalysisofthebeclin1expressionpredictstheprogressionoforalsquamouscellcarcinoma
_version_	1718432369411620864

Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma

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