The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients

Abstract Fixed orthodontic appliances are common and effective tools to treat malocclusion. Adverse effects of these appliances, such as dental caries and periodontitis, may be associated with alteration of the microbiome. This study investigated the impact of these appliances on the dynamics of the...

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Autores principales:	Isamu Kado, Junzo Hisatsune, Keiko Tsuruda, Kotaro Tanimoto, Motoyuki Sugai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9090bdfe15c34647a1c9ad3fd333d2ff
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spelling	oai:doaj.org-article:9090bdfe15c34647a1c9ad3fd333d2ff2021-12-02T13:34:01ZThe impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients10.1038/s41598-020-78971-22045-2322https://doaj.org/article/9090bdfe15c34647a1c9ad3fd333d2ff2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78971-2https://doaj.org/toc/2045-2322Abstract Fixed orthodontic appliances are common and effective tools to treat malocclusion. Adverse effects of these appliances, such as dental caries and periodontitis, may be associated with alteration of the microbiome. This study investigated the impact of these appliances on the dynamics of the oral microbiome. Seventy-one patients were selected. Supragingival plaque samples were collected before placement (T0) and six months after placement (T1). Saliva samples were collected at T0 and T1, and then when appliance removal (T2). Microbial DNA was analyzed by 16S rRNA meta-sequencing. The diversity analysis indicated dynamic changes in the structure of the oral microbiome. Taxonomic analysis at phylum level showed a significant increase in Bacteroidetes and Saccharibacteria (formally TM7) and decrease in Proteobacteria and Actinobacteria over time, in both plaque and saliva. Genus level analysis of relative abundance indicated a significant increase in anaerobic and facultative anaerobes in both plaque and saliva. Fixed orthodontic appliances induced measurable changes in the oral microbiome. This was characterized by an increase in relative abundance of obligate anaerobes, including periodontal pathogens. It can be concluded that this dysbiosis induced by fixed orthodontic appliances is likely to represent a transitional stage in the shift in microbiome from healthy to periodontitis.Isamu KadoJunzo HisatsuneKeiko TsurudaKotaro TanimotoMotoyuki SugaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Isamu Kado Junzo Hisatsune Keiko Tsuruda Kotaro Tanimoto Motoyuki Sugai The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients
description	Abstract Fixed orthodontic appliances are common and effective tools to treat malocclusion. Adverse effects of these appliances, such as dental caries and periodontitis, may be associated with alteration of the microbiome. This study investigated the impact of these appliances on the dynamics of the oral microbiome. Seventy-one patients were selected. Supragingival plaque samples were collected before placement (T0) and six months after placement (T1). Saliva samples were collected at T0 and T1, and then when appliance removal (T2). Microbial DNA was analyzed by 16S rRNA meta-sequencing. The diversity analysis indicated dynamic changes in the structure of the oral microbiome. Taxonomic analysis at phylum level showed a significant increase in Bacteroidetes and Saccharibacteria (formally TM7) and decrease in Proteobacteria and Actinobacteria over time, in both plaque and saliva. Genus level analysis of relative abundance indicated a significant increase in anaerobic and facultative anaerobes in both plaque and saliva. Fixed orthodontic appliances induced measurable changes in the oral microbiome. This was characterized by an increase in relative abundance of obligate anaerobes, including periodontal pathogens. It can be concluded that this dysbiosis induced by fixed orthodontic appliances is likely to represent a transitional stage in the shift in microbiome from healthy to periodontitis.
format	article
author	Isamu Kado Junzo Hisatsune Keiko Tsuruda Kotaro Tanimoto Motoyuki Sugai
author_facet	Isamu Kado Junzo Hisatsune Keiko Tsuruda Kotaro Tanimoto Motoyuki Sugai
author_sort	Isamu Kado
title	The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients
title_short	The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients
title_full	The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients
title_fullStr	The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients
title_full_unstemmed	The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients
title_sort	impact of fixed orthodontic appliances on oral microbiome dynamics in japanese patients
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9090bdfe15c34647a1c9ad3fd333d2ff
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The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients

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