Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen

Stomata in the plant epidermis open in response to light and regulate CO2 uptake for photosynthesis and transpiration for uptake of water and nutrients from roots. Light-induced stomatal opening is mediated by activation of the plasma membrane (PM) H+-ATPase in guard cells. Overexpression of PM H+-A...

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Autores principales:	Shigeo Toh, Naoki Takata, Eigo Ando, Yosuke Toda, Yin Wang, Yuki Hayashi, Nobutaka Mitsuda, Soichiro Nagano, Toru Taniguchi, Toshinori Kinoshita
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	PUMP PM H+-ATPase guard cell stomatal conductance hybrid aspen Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/9096d1cecd5c452b81701b0c929622ff
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spelling	oai:doaj.org-article:9096d1cecd5c452b81701b0c929622ff2021-12-01T09:04:20ZOverexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen1664-462X10.3389/fpls.2021.766037https://doaj.org/article/9096d1cecd5c452b81701b0c929622ff2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.766037/fullhttps://doaj.org/toc/1664-462XStomata in the plant epidermis open in response to light and regulate CO2 uptake for photosynthesis and transpiration for uptake of water and nutrients from roots. Light-induced stomatal opening is mediated by activation of the plasma membrane (PM) H+-ATPase in guard cells. Overexpression of PM H+-ATPase in guard cells promotes light-induced stomatal opening, enhancing photosynthesis and growth in Arabidopsis thaliana. In this study, transgenic hybrid aspens overexpressing Arabidopsis PM H+-ATPase (AHA2) in guard cells under the strong guard cell promoter Arabidopsis GC1 (AtGC1) showed enhanced light-induced stomatal opening, photosynthesis, and growth. First, we confirmed that AtGC1 induces GUS expression specifically in guard cells in hybrid aspens. Thus, we produced AtGC1::AHA2 transgenic hybrid aspens and confirmed expression of AHA2 in AtGC1::AHA2 transgenic plants. In addition, AtGC1::AHA2 transgenic plants showed a higher PM H+-ATPase protein level in guard cells. Analysis using a gas exchange system revealed that transpiration and the photosynthetic rate were significantly increased in AtGC1::AHA2 transgenic aspen plants. AtGC1::AHA2 transgenic plants showed a>20% higher stem elongation rate than the wild type (WT). Therefore, overexpression of PM H+-ATPase in guard cells promotes the growth of perennial woody plants.Shigeo TohShigeo TohNaoki TakataEigo AndoEigo AndoYosuke TodaYosuke TodaYosuke TodaYin WangYin WangYuki HayashiNobutaka MitsudaNobutaka MitsudaSoichiro NaganoToru TaniguchiToru TaniguchiToshinori KinoshitaToshinori KinoshitaFrontiers Media S.A.articlePUMPPM H+-ATPaseguard cellstomatal conductancehybrid aspenPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PUMP PM H+-ATPase guard cell stomatal conductance hybrid aspen Plant culture SB1-1110
spellingShingle	PUMP PM H+-ATPase guard cell stomatal conductance hybrid aspen Plant culture SB1-1110 Shigeo Toh Shigeo Toh Naoki Takata Eigo Ando Eigo Ando Yosuke Toda Yosuke Toda Yosuke Toda Yin Wang Yin Wang Yuki Hayashi Nobutaka Mitsuda Nobutaka Mitsuda Soichiro Nagano Toru Taniguchi Toru Taniguchi Toshinori Kinoshita Toshinori Kinoshita Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen
description	Stomata in the plant epidermis open in response to light and regulate CO2 uptake for photosynthesis and transpiration for uptake of water and nutrients from roots. Light-induced stomatal opening is mediated by activation of the plasma membrane (PM) H+-ATPase in guard cells. Overexpression of PM H+-ATPase in guard cells promotes light-induced stomatal opening, enhancing photosynthesis and growth in Arabidopsis thaliana. In this study, transgenic hybrid aspens overexpressing Arabidopsis PM H+-ATPase (AHA2) in guard cells under the strong guard cell promoter Arabidopsis GC1 (AtGC1) showed enhanced light-induced stomatal opening, photosynthesis, and growth. First, we confirmed that AtGC1 induces GUS expression specifically in guard cells in hybrid aspens. Thus, we produced AtGC1::AHA2 transgenic hybrid aspens and confirmed expression of AHA2 in AtGC1::AHA2 transgenic plants. In addition, AtGC1::AHA2 transgenic plants showed a higher PM H+-ATPase protein level in guard cells. Analysis using a gas exchange system revealed that transpiration and the photosynthetic rate were significantly increased in AtGC1::AHA2 transgenic aspen plants. AtGC1::AHA2 transgenic plants showed a>20% higher stem elongation rate than the wild type (WT). Therefore, overexpression of PM H+-ATPase in guard cells promotes the growth of perennial woody plants.
format	article
author	Shigeo Toh Shigeo Toh Naoki Takata Eigo Ando Eigo Ando Yosuke Toda Yosuke Toda Yosuke Toda Yin Wang Yin Wang Yuki Hayashi Nobutaka Mitsuda Nobutaka Mitsuda Soichiro Nagano Toru Taniguchi Toru Taniguchi Toshinori Kinoshita Toshinori Kinoshita
author_facet	Shigeo Toh Shigeo Toh Naoki Takata Eigo Ando Eigo Ando Yosuke Toda Yosuke Toda Yosuke Toda Yin Wang Yin Wang Yuki Hayashi Nobutaka Mitsuda Nobutaka Mitsuda Soichiro Nagano Toru Taniguchi Toru Taniguchi Toshinori Kinoshita Toshinori Kinoshita
author_sort	Shigeo Toh
title	Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen
title_short	Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen
title_full	Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen
title_fullStr	Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen
title_full_unstemmed	Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen
title_sort	overexpression of plasma membrane h+-atpase in guard cells enhances light-induced stomatal opening, photosynthesis, and plant growth in hybrid aspen
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9096d1cecd5c452b81701b0c929622ff
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Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen

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