Characterization of the <named-content content-type="genus-species">Candida albicans</named-content> Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an <italic toggle="yes">S</italic>-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis

ABSTRACT Amino acids are key sources of nitrogen for growth of Candida albicans. In order to detect and take up these amino acids from a broad range of different and changing nitrogen sources inside the host, this fungus must be able to adapt via its expression of genes for amino acid uptake and fur...

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Autores principales:	Lucie Kraidlova, Sanne Schrevens, Hélène Tournu, Griet Van Zeebroeck, Hana Sychrova, Patrick Van Dijck
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Candida albicans GAP1 S-adenosyl methionine general amino acid permease morphogenesis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/90988c607f8d4748ab224298c325c751
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spelling	oai:doaj.org-article:90988c607f8d4748ab224298c325c7512021-11-15T15:22:03ZCharacterization of the <named-content content-type="genus-species">Candida albicans</named-content> Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an <italic toggle="yes">S</italic>-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis10.1128/mSphere.00284-162379-5042https://doaj.org/article/90988c607f8d4748ab224298c325c7512016-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00284-16https://doaj.org/toc/2379-5042ABSTRACT Amino acids are key sources of nitrogen for growth of Candida albicans. In order to detect and take up these amino acids from a broad range of different and changing nitrogen sources inside the host, this fungus must be able to adapt via its expression of genes for amino acid uptake and further metabolism. We analyzed six C. albicans putative general amino acid permeases based on their homology to the Saccharomyces cerevisiae Gap1 general amino acid permease. We generated single- and multiple-deletion strains and found that, based on growth assays and transcriptional or posttranscriptional regulation, Gap2 is the functional orthologue to ScGap1, with broad substrate specificity. Expression analysis showed that expression of all GAP genes is under control of the Csy1 amino acid sensor, which is different from the situation in S. cerevisiae, where the expression of ScGAP1 is not regulated by Ssy1. We show that Gap4 is the functional orthologue of ScSam3, the only S-adenosylmethionine (SAM) transporter in S. cerevisiae, and we report that Gap4 is required for SAM-induced morphogenesis. IMPORTANCE Candida albicans is a commensal organism that can thrive in many niches in its human host. The environmental conditions at these different niches differ quite a bit, and this fungus must be able to sense these changes and adapt its metabolism to them. Apart from glucose and other sugars, the uptake of amino acids is very important. This is underscored by the fact that the C. albicans genome encodes 6 orthologues of the Saccharomyces. cerevisiae general amino acid permease Gap1 and many other amino acid transporters. In this work, we characterize these six permeases and we show that C. albicans Gap2 is the functional orthologue of ScGap1 and that C. albicans Gap4 is an orthologue of ScSam3, an S-adenosylmethionine (SAM) transporter. Furthermore, we show that Gap4 is required for SAM-induced morphogenesis, an important virulence factor of C. albicans.Lucie KraidlovaSanne SchrevensHélène TournuGriet Van ZeebroeckHana SychrovaPatrick Van DijckAmerican Society for MicrobiologyarticleCandida albicansGAP1S-adenosyl methioninegeneral amino acid permeasemorphogenesisMicrobiologyQR1-502ENmSphere, Vol 1, Iss 6 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida albicans GAP1 S-adenosyl methionine general amino acid permease morphogenesis Microbiology QR1-502
spellingShingle	Candida albicans GAP1 S-adenosyl methionine general amino acid permease morphogenesis Microbiology QR1-502 Lucie Kraidlova Sanne Schrevens Hélène Tournu Griet Van Zeebroeck Hana Sychrova Patrick Van Dijck Characterization of the <named-content content-type="genus-species">Candida albicans</named-content> Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an <italic toggle="yes">S</italic>-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis
description	ABSTRACT Amino acids are key sources of nitrogen for growth of Candida albicans. In order to detect and take up these amino acids from a broad range of different and changing nitrogen sources inside the host, this fungus must be able to adapt via its expression of genes for amino acid uptake and further metabolism. We analyzed six C. albicans putative general amino acid permeases based on their homology to the Saccharomyces cerevisiae Gap1 general amino acid permease. We generated single- and multiple-deletion strains and found that, based on growth assays and transcriptional or posttranscriptional regulation, Gap2 is the functional orthologue to ScGap1, with broad substrate specificity. Expression analysis showed that expression of all GAP genes is under control of the Csy1 amino acid sensor, which is different from the situation in S. cerevisiae, where the expression of ScGAP1 is not regulated by Ssy1. We show that Gap4 is the functional orthologue of ScSam3, the only S-adenosylmethionine (SAM) transporter in S. cerevisiae, and we report that Gap4 is required for SAM-induced morphogenesis. IMPORTANCE Candida albicans is a commensal organism that can thrive in many niches in its human host. The environmental conditions at these different niches differ quite a bit, and this fungus must be able to sense these changes and adapt its metabolism to them. Apart from glucose and other sugars, the uptake of amino acids is very important. This is underscored by the fact that the C. albicans genome encodes 6 orthologues of the Saccharomyces. cerevisiae general amino acid permease Gap1 and many other amino acid transporters. In this work, we characterize these six permeases and we show that C. albicans Gap2 is the functional orthologue of ScGap1 and that C. albicans Gap4 is an orthologue of ScSam3, an S-adenosylmethionine (SAM) transporter. Furthermore, we show that Gap4 is required for SAM-induced morphogenesis, an important virulence factor of C. albicans.
format	article
author	Lucie Kraidlova Sanne Schrevens Hélène Tournu Griet Van Zeebroeck Hana Sychrova Patrick Van Dijck
author_facet	Lucie Kraidlova Sanne Schrevens Hélène Tournu Griet Van Zeebroeck Hana Sychrova Patrick Van Dijck
author_sort	Lucie Kraidlova
title	Characterization of the <named-content content-type="genus-species">Candida albicans</named-content> Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an <italic toggle="yes">S</italic>-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis
title_short	Characterization of the <named-content content-type="genus-species">Candida albicans</named-content> Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an <italic toggle="yes">S</italic>-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis
title_full	Characterization of the <named-content content-type="genus-species">Candida albicans</named-content> Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an <italic toggle="yes">S</italic>-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis
title_fullStr	Characterization of the <named-content content-type="genus-species">Candida albicans</named-content> Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an <italic toggle="yes">S</italic>-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis
title_full_unstemmed	Characterization of the <named-content content-type="genus-species">Candida albicans</named-content> Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an <italic toggle="yes">S</italic>-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis
title_sort	characterization of the <named-content content-type="genus-species">candida albicans</named-content> amino acid permease family: gap2 is the only general amino acid permease and gap4 is an <italic toggle="yes">s</italic>-adenosylmethionine (sam) transporter required for sam-induced morphogenesis
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/90988c607f8d4748ab224298c325c751
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Characterization of the <named-content content-type="genus-species">Candida albicans</named-content> Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an <italic toggle="yes">S</italic>-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis

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