EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration

Abstract Age-related macular degeneration (AMD) is a highly prevalent degenerative disease and a leading cause of vision loss worldwide. Evidence for an inflammatory component in the development of AMD exists, yet the exact mechanisms remain unclear. Bisretinoid N-retinylidene-N-retinylethanolamine...

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Autores principales:	Jon Ambæk Durhuus, Maarten P. Rozing, Marie Krogh Nielsen, Christopher Rue Molbech, Guido Keijzers, Morten Scheibye-Knudsen, Lene Juel Rasmussen, Rudi G. J. Westendorp, Torben Lykke Sørensen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/90a31db0e9514fae98b0ee165a94d82a
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spelling	oai:doaj.org-article:90a31db0e9514fae98b0ee165a94d82a2021-12-02T14:30:46ZEX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration10.1038/s41598-021-87337-12045-2322https://doaj.org/article/90a31db0e9514fae98b0ee165a94d82a2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87337-1https://doaj.org/toc/2045-2322Abstract Age-related macular degeneration (AMD) is a highly prevalent degenerative disease and a leading cause of vision loss worldwide. Evidence for an inflammatory component in the development of AMD exists, yet the exact mechanisms remain unclear. Bisretinoid N-retinylidene-N-retinylethanolamine (A2E) in retinal pigmental epithelial (RPE) cells, and in extracellular deposits constitutes a hallmark of AMD, but its role in the pathology of AMD is elusive. Here, we tested the hypothesis that A2E is responsible for the heightened inflammatory activity in AMD. To this end, we measured ex vivo mRNA expression of the cytokines TNF-α, IL-6, and IL-10 in whole blood samples after stimulation with A2E in a clinical sample of 27 patients with neovascular AMD and 24 patients with geographic atrophy secondary to AMD. Patients’ spouses (n = 30) were included as non-affected controls. After stimulation with A2E, no statistical differences were found in the median expression level of TNF-α, IL-6, IL-10 between the control group, and the neovascular AMD and the geographic atrophy group. Our findings do not support evidence for the hypothesis, that A2E per se contributes to heightened inflammatory activity in AMD.Jon Ambæk DurhuusMaarten P. RozingMarie Krogh NielsenChristopher Rue MolbechGuido KeijzersMorten Scheibye-KnudsenLene Juel RasmussenRudi G. J. WestendorpTorben Lykke SørensenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jon Ambæk Durhuus Maarten P. Rozing Marie Krogh Nielsen Christopher Rue Molbech Guido Keijzers Morten Scheibye-Knudsen Lene Juel Rasmussen Rudi G. J. Westendorp Torben Lykke Sørensen EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
description	Abstract Age-related macular degeneration (AMD) is a highly prevalent degenerative disease and a leading cause of vision loss worldwide. Evidence for an inflammatory component in the development of AMD exists, yet the exact mechanisms remain unclear. Bisretinoid N-retinylidene-N-retinylethanolamine (A2E) in retinal pigmental epithelial (RPE) cells, and in extracellular deposits constitutes a hallmark of AMD, but its role in the pathology of AMD is elusive. Here, we tested the hypothesis that A2E is responsible for the heightened inflammatory activity in AMD. To this end, we measured ex vivo mRNA expression of the cytokines TNF-α, IL-6, and IL-10 in whole blood samples after stimulation with A2E in a clinical sample of 27 patients with neovascular AMD and 24 patients with geographic atrophy secondary to AMD. Patients’ spouses (n = 30) were included as non-affected controls. After stimulation with A2E, no statistical differences were found in the median expression level of TNF-α, IL-6, IL-10 between the control group, and the neovascular AMD and the geographic atrophy group. Our findings do not support evidence for the hypothesis, that A2E per se contributes to heightened inflammatory activity in AMD.
format	article
author	Jon Ambæk Durhuus Maarten P. Rozing Marie Krogh Nielsen Christopher Rue Molbech Guido Keijzers Morten Scheibye-Knudsen Lene Juel Rasmussen Rudi G. J. Westendorp Torben Lykke Sørensen
author_facet	Jon Ambæk Durhuus Maarten P. Rozing Marie Krogh Nielsen Christopher Rue Molbech Guido Keijzers Morten Scheibye-Knudsen Lene Juel Rasmussen Rudi G. J. Westendorp Torben Lykke Sørensen
author_sort	Jon Ambæk Durhuus
title	EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
title_short	EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
title_full	EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
title_fullStr	EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
title_full_unstemmed	EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
title_sort	ex-vivo whole blood stimulation with a2e does not elicit an inflammatory cytokine response in patients with age-related macular degeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/90a31db0e9514fae98b0ee165a94d82a
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EX-vivo whole blood stimulation with A2E does not elicit an inflammatory cytokine response in patients with age-related macular degeneration

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