Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.

The clinical course of Staphylococcus aureus bacteremia varies extensively. We sought to determine the relationship between genetic characteristics of the infecting pathogen and clinical outcomes in an exploratory study. In two study centers, 317 blood culture isolates were analyzed by DNA microarra...

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Autores principales: Siegbert Rieg, Daniel Jonas, Achim J Kaasch, Christine Porzelius, Gabriele Peyerl-Hoffmann, Christian Theilacker, Marc-Fabian Küpper, Christian Schneider, Harald Seifert, Winfried V Kern
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Publicado: Public Library of Science (PLoS) 2013
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spelling oai:doaj.org-article:90a6399ad61e452db29bb3d72fc0b8512021-11-18T08:59:41ZMicroarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.1932-620310.1371/journal.pone.0071259https://doaj.org/article/90a6399ad61e452db29bb3d72fc0b8512013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23967176/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The clinical course of Staphylococcus aureus bacteremia varies extensively. We sought to determine the relationship between genetic characteristics of the infecting pathogen and clinical outcomes in an exploratory study. In two study centers, 317 blood culture isolates were analyzed by DNA microarray and spa genotyping. By uni- and multivariate regression analyses associations of genotype data with 30-day all-cause mortality, severe sepsis/septic shock, disseminated disease, endocarditis, and osteoarticular infection were investigated. Univariate analysis showed significant association between S. aureus genes/gene-clusters or clonal complexes and clinical endpoints. For example CC15 was associated with 30-day mortality and CC22 with osteoarticular infection. In multivariate analysis methicillin resistance (mecA, OR 4.8 [1.43-16.06]) and the beta-lactamase-gene (bla, OR 3.12 [1.17-8.30]) remained independently associated with 30-day mortality. The presence of genes for enterotoxins (sed/sej/ser) was associated with endocarditis (OR 5.11 [1.14-18.62]). Host factors such as McCabe classification (OR 4.52 [2.09-9.79] for mortality), age (OR 1.06 [1.03-1.10] per year), and community-acquisition (OR 3.40 [1.31-8.81]) had a major influence on disease severity, dissemination and mortality. Individual genotypes and clonal complexes of S. aureus can only partially explain clinical features and outcomes of S. aureus bacteremia. Genotype-phenotype association studies need to include adjustments for host factors like age, comorbidity and community-acquisition.Siegbert RiegDaniel JonasAchim J KaaschChristine PorzeliusGabriele Peyerl-HoffmannChristian TheilackerMarc-Fabian KüpperChristian SchneiderHarald SeifertWinfried V KernPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71259 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Siegbert Rieg
Daniel Jonas
Achim J Kaasch
Christine Porzelius
Gabriele Peyerl-Hoffmann
Christian Theilacker
Marc-Fabian Küpper
Christian Schneider
Harald Seifert
Winfried V Kern
Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.
description The clinical course of Staphylococcus aureus bacteremia varies extensively. We sought to determine the relationship between genetic characteristics of the infecting pathogen and clinical outcomes in an exploratory study. In two study centers, 317 blood culture isolates were analyzed by DNA microarray and spa genotyping. By uni- and multivariate regression analyses associations of genotype data with 30-day all-cause mortality, severe sepsis/septic shock, disseminated disease, endocarditis, and osteoarticular infection were investigated. Univariate analysis showed significant association between S. aureus genes/gene-clusters or clonal complexes and clinical endpoints. For example CC15 was associated with 30-day mortality and CC22 with osteoarticular infection. In multivariate analysis methicillin resistance (mecA, OR 4.8 [1.43-16.06]) and the beta-lactamase-gene (bla, OR 3.12 [1.17-8.30]) remained independently associated with 30-day mortality. The presence of genes for enterotoxins (sed/sej/ser) was associated with endocarditis (OR 5.11 [1.14-18.62]). Host factors such as McCabe classification (OR 4.52 [2.09-9.79] for mortality), age (OR 1.06 [1.03-1.10] per year), and community-acquisition (OR 3.40 [1.31-8.81]) had a major influence on disease severity, dissemination and mortality. Individual genotypes and clonal complexes of S. aureus can only partially explain clinical features and outcomes of S. aureus bacteremia. Genotype-phenotype association studies need to include adjustments for host factors like age, comorbidity and community-acquisition.
format article
author Siegbert Rieg
Daniel Jonas
Achim J Kaasch
Christine Porzelius
Gabriele Peyerl-Hoffmann
Christian Theilacker
Marc-Fabian Küpper
Christian Schneider
Harald Seifert
Winfried V Kern
author_facet Siegbert Rieg
Daniel Jonas
Achim J Kaasch
Christine Porzelius
Gabriele Peyerl-Hoffmann
Christian Theilacker
Marc-Fabian Küpper
Christian Schneider
Harald Seifert
Winfried V Kern
author_sort Siegbert Rieg
title Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.
title_short Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.
title_full Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.
title_fullStr Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.
title_full_unstemmed Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.
title_sort microarray-based genotyping and clinical outcomes of staphylococcus aureus bloodstream infection: an exploratory study.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/90a6399ad61e452db29bb3d72fc0b851
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