Pannexin-1 channel opening is critical for COVID-19 pathogenesis

Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly rampaged worldwide, causing a pandemic of coronavirus disease (COVID -19), but the biology of SARS-CoV-2 remains under investigation. We demonstrate that both SARS-CoV-2 spike protein and human coronavirus 229E (hCoV-229E)...

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Autores principales:	Ross Luu, Silvana Valdebenito, Eliana Scemes, Antonio Cibelli, David C. Spray, Maximiliano Rovegno, Juan Tichauer, Andrea Cottignies-Calamarte, Arielle Rosenberg, Calude Capron, Sandrine Belouzard, Jean Dubuisson, Djillali Annane, Geoffroy Lorin de la Grandmaison, Elisabeth Cramer-Bordé, Morgane Bomsel, Eliseo Eugenin
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell biology Molecular physiology Virology Science Q
Acceso en línea:	https://doaj.org/article/90aa3a5ba1df4dd5a1cd73c53a82493c
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spelling	oai:doaj.org-article:90aa3a5ba1df4dd5a1cd73c53a82493c2021-12-04T04:35:33ZPannexin-1 channel opening is critical for COVID-19 pathogenesis2589-004210.1016/j.isci.2021.103478https://doaj.org/article/90aa3a5ba1df4dd5a1cd73c53a82493c2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221014498https://doaj.org/toc/2589-0042Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly rampaged worldwide, causing a pandemic of coronavirus disease (COVID -19), but the biology of SARS-CoV-2 remains under investigation. We demonstrate that both SARS-CoV-2 spike protein and human coronavirus 229E (hCoV-229E) or its purified S protein, one of the main viruses responsible for the common cold, induce the transient opening of Pannexin-1 (Panx-1) channels in human lung epithelial cells. However, the Panx-1 channel opening induced by SARS-CoV-2 is greater and more prolonged than hCoV-229E/S protein, resulting in an enhanced ATP, PGE2, and IL-1β release. Analysis of lung lavages and tissues indicate that Panx-1 mRNA expression is associated with increased ATP, PGE2, and IL-1β levels. Panx-1 channel opening induced by SARS-CoV-2 spike protein is angiotensin-converting enzyme 2 (ACE-2), endocytosis, and furin dependent. Overall, we demonstrated that Panx-1 channel is a critical contributor to SARS-CoV-2 infection and should be considered as an alternative therapy.Ross LuuSilvana ValdebenitoEliana ScemesAntonio CibelliDavid C. SprayMaximiliano RovegnoJuan TichauerAndrea Cottignies-CalamarteArielle RosenbergCalude CapronSandrine BelouzardJean DubuissonDjillali AnnaneGeoffroy Lorin de la GrandmaisonElisabeth Cramer-BordéMorgane BomselEliseo EugeninElsevierarticleCell biologyMolecular physiologyVirologyScienceQENiScience, Vol 24, Iss 12, Pp 103478- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell biology Molecular physiology Virology Science Q
spellingShingle	Cell biology Molecular physiology Virology Science Q Ross Luu Silvana Valdebenito Eliana Scemes Antonio Cibelli David C. Spray Maximiliano Rovegno Juan Tichauer Andrea Cottignies-Calamarte Arielle Rosenberg Calude Capron Sandrine Belouzard Jean Dubuisson Djillali Annane Geoffroy Lorin de la Grandmaison Elisabeth Cramer-Bordé Morgane Bomsel Eliseo Eugenin Pannexin-1 channel opening is critical for COVID-19 pathogenesis
description	Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly rampaged worldwide, causing a pandemic of coronavirus disease (COVID -19), but the biology of SARS-CoV-2 remains under investigation. We demonstrate that both SARS-CoV-2 spike protein and human coronavirus 229E (hCoV-229E) or its purified S protein, one of the main viruses responsible for the common cold, induce the transient opening of Pannexin-1 (Panx-1) channels in human lung epithelial cells. However, the Panx-1 channel opening induced by SARS-CoV-2 is greater and more prolonged than hCoV-229E/S protein, resulting in an enhanced ATP, PGE2, and IL-1β release. Analysis of lung lavages and tissues indicate that Panx-1 mRNA expression is associated with increased ATP, PGE2, and IL-1β levels. Panx-1 channel opening induced by SARS-CoV-2 spike protein is angiotensin-converting enzyme 2 (ACE-2), endocytosis, and furin dependent. Overall, we demonstrated that Panx-1 channel is a critical contributor to SARS-CoV-2 infection and should be considered as an alternative therapy.
format	article
author	Ross Luu Silvana Valdebenito Eliana Scemes Antonio Cibelli David C. Spray Maximiliano Rovegno Juan Tichauer Andrea Cottignies-Calamarte Arielle Rosenberg Calude Capron Sandrine Belouzard Jean Dubuisson Djillali Annane Geoffroy Lorin de la Grandmaison Elisabeth Cramer-Bordé Morgane Bomsel Eliseo Eugenin
author_facet	Ross Luu Silvana Valdebenito Eliana Scemes Antonio Cibelli David C. Spray Maximiliano Rovegno Juan Tichauer Andrea Cottignies-Calamarte Arielle Rosenberg Calude Capron Sandrine Belouzard Jean Dubuisson Djillali Annane Geoffroy Lorin de la Grandmaison Elisabeth Cramer-Bordé Morgane Bomsel Eliseo Eugenin
author_sort	Ross Luu
title	Pannexin-1 channel opening is critical for COVID-19 pathogenesis
title_short	Pannexin-1 channel opening is critical for COVID-19 pathogenesis
title_full	Pannexin-1 channel opening is critical for COVID-19 pathogenesis
title_fullStr	Pannexin-1 channel opening is critical for COVID-19 pathogenesis
title_full_unstemmed	Pannexin-1 channel opening is critical for COVID-19 pathogenesis
title_sort	pannexin-1 channel opening is critical for covid-19 pathogenesis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/90aa3a5ba1df4dd5a1cd73c53a82493c
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Pannexin-1 channel opening is critical for COVID-19 pathogenesis

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