The interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.

One of the hallmarks of Alzheimer's disease is the formation of senile plaques, primarily consisting of amyloid-β (Aβ) peptides. Peptide-membrane and peptide-lipid interactions are thought to be crucial in this process. We studied the interaction of Aβ₁₋₄₂ and Aβ₂₅₋₃₅ peptides with anionic lipi...

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Autores principales:	Hannah Dies, Laura Toppozini, Maikel C Rheinstädter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/90ad7f8017df421d88510a72569bc2a0
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spelling	oai:doaj.org-article:90ad7f8017df421d88510a72569bc2a02021-11-18T08:16:16ZThe interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.1932-620310.1371/journal.pone.0099124https://doaj.org/article/90ad7f8017df421d88510a72569bc2a02014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24915524/?tool=EBIhttps://doaj.org/toc/1932-6203One of the hallmarks of Alzheimer's disease is the formation of senile plaques, primarily consisting of amyloid-β (Aβ) peptides. Peptide-membrane and peptide-lipid interactions are thought to be crucial in this process. We studied the interaction of Aβ₁₋₄₂ and Aβ₂₅₋₃₅ peptides with anionic lipid membranes made of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphoserine (DMPS) using X-ray diffraction. We compare the experimentally determined electron densities in the gel state of the membranes with density calculations from peptide structures reported in the Protein Data Bank in order to determine the position of the peptide in the bilayers. The full length peptide Aβ₁₋₄₂ was found to embed in the hydrocarbon core of the anionic lipid bilayers. Two populations were found for the Aβ₂₅₋₃₅ peptide: (1) membrane-bound states in the hydrophilic head group region of the bilayers, where the peptides align parallel to the membranes, and (2) an embedded state in the bilayer center. Aging plays an important role in the development of Alzheimer's, in particular with respect to changes in cholesterol and melatonin levels in the brain tissue. Immiscible cholesterol plaques were created by addition of 30 mol% cholesterol to the anionic membranes. The Aβ₂₅₋₃₅ peptides were found to strongly interact with the lipid bilayers, displacing further cholesterol molecules into the plaques, effectively lowering the cholesterol concentration in the membranes and increasing the total fraction of cholesterol plaques. Addition of 30 mol% melatonin molecules to the anionic membranes drastically reduced the population of the membrane-embedded Aβ state. These results present experimental evidence for an interaction between Aβ peptides, melatonin and cholesterol in lipid membranes.Hannah DiesLaura ToppoziniMaikel C RheinstädterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99124 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hannah Dies Laura Toppozini Maikel C Rheinstädter The interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.
description	One of the hallmarks of Alzheimer's disease is the formation of senile plaques, primarily consisting of amyloid-β (Aβ) peptides. Peptide-membrane and peptide-lipid interactions are thought to be crucial in this process. We studied the interaction of Aβ₁₋₄₂ and Aβ₂₅₋₃₅ peptides with anionic lipid membranes made of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphoserine (DMPS) using X-ray diffraction. We compare the experimentally determined electron densities in the gel state of the membranes with density calculations from peptide structures reported in the Protein Data Bank in order to determine the position of the peptide in the bilayers. The full length peptide Aβ₁₋₄₂ was found to embed in the hydrocarbon core of the anionic lipid bilayers. Two populations were found for the Aβ₂₅₋₃₅ peptide: (1) membrane-bound states in the hydrophilic head group region of the bilayers, where the peptides align parallel to the membranes, and (2) an embedded state in the bilayer center. Aging plays an important role in the development of Alzheimer's, in particular with respect to changes in cholesterol and melatonin levels in the brain tissue. Immiscible cholesterol plaques were created by addition of 30 mol% cholesterol to the anionic membranes. The Aβ₂₅₋₃₅ peptides were found to strongly interact with the lipid bilayers, displacing further cholesterol molecules into the plaques, effectively lowering the cholesterol concentration in the membranes and increasing the total fraction of cholesterol plaques. Addition of 30 mol% melatonin molecules to the anionic membranes drastically reduced the population of the membrane-embedded Aβ state. These results present experimental evidence for an interaction between Aβ peptides, melatonin and cholesterol in lipid membranes.
format	article
author	Hannah Dies Laura Toppozini Maikel C Rheinstädter
author_facet	Hannah Dies Laura Toppozini Maikel C Rheinstädter
author_sort	Hannah Dies
title	The interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.
title_short	The interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.
title_full	The interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.
title_fullStr	The interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.
title_full_unstemmed	The interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.
title_sort	interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/90ad7f8017df421d88510a72569bc2a0
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The interaction between amyloid-β peptides and anionic lipid membranes containing cholesterol and melatonin.

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