Perinatal exposure to low-dose methoxychlor impairs testicular development in C57BL/6 mice.

Methoxychlor (MXC), an organochlorine pesticide, has adverse effects on male reproduction at toxicological doses. Humans and wild animals are exposed to MXC mostly through contaminated dietary intake. Higher concentrations of MXC have been found in human milk, raising the demand for the risk assessm...

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Autores principales:	Xiaohong Du, Hua Zhang, Yuanwu Liu, Wanpeng Yu, Chaobin Huang, Xiangdong Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/90b0d9c0b04b4260b06a8992b9a65eb9
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spelling	oai:doaj.org-article:90b0d9c0b04b4260b06a8992b9a65eb92021-11-25T06:07:45ZPerinatal exposure to low-dose methoxychlor impairs testicular development in C57BL/6 mice.1932-620310.1371/journal.pone.0103016https://doaj.org/article/90b0d9c0b04b4260b06a8992b9a65eb92014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25048109/?tool=EBIhttps://doaj.org/toc/1932-6203Methoxychlor (MXC), an organochlorine pesticide, has adverse effects on male reproduction at toxicological doses. Humans and wild animals are exposed to MXC mostly through contaminated dietary intake. Higher concentrations of MXC have been found in human milk, raising the demand for the risk assessment of offspring after maternal exposure to low doses of MXC. In this study, pregnant mice (F0) were given intraperitoneal daily evening injections of 1 mg/kg/d MXC during their gestational (embryonic day 0.5, E0.5) and lactational periods (postnatal day 21.5, P21.5), and the F1 males were assessed. F1 testes were collected at P0.5, P21.5 and P45.5. Maternal exposure to MXC disturbed the testicular development. Serum testosterone levels decreased, whereas estradiol levels increased. To understand the molecular mechanisms of exposure to MXC in male reproduction, the F1 testes were examined for changes in the expression of steroidogenesis- and spermatogenesis- related genes. RT-PCR analysis demonstrated that MXC significantly decreased Cyp11a1 and increased Cyp19a1; furthermore, it downregulated certain spermatogenic genes (Dazl, Boll, Rarg, Stra8 and Cyclin-a1). In summary, perinatal exposure to low-dose MXC disturbs the testicular development in mice. This animal study of exposure to low-dose MXC in F1 males suggests similar dysfunctional effects on male reproduction in humans.Xiaohong DuHua ZhangYuanwu LiuWanpeng YuChaobin HuangXiangdong LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e103016 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaohong Du Hua Zhang Yuanwu Liu Wanpeng Yu Chaobin Huang Xiangdong Li Perinatal exposure to low-dose methoxychlor impairs testicular development in C57BL/6 mice.
description	Methoxychlor (MXC), an organochlorine pesticide, has adverse effects on male reproduction at toxicological doses. Humans and wild animals are exposed to MXC mostly through contaminated dietary intake. Higher concentrations of MXC have been found in human milk, raising the demand for the risk assessment of offspring after maternal exposure to low doses of MXC. In this study, pregnant mice (F0) were given intraperitoneal daily evening injections of 1 mg/kg/d MXC during their gestational (embryonic day 0.5, E0.5) and lactational periods (postnatal day 21.5, P21.5), and the F1 males were assessed. F1 testes were collected at P0.5, P21.5 and P45.5. Maternal exposure to MXC disturbed the testicular development. Serum testosterone levels decreased, whereas estradiol levels increased. To understand the molecular mechanisms of exposure to MXC in male reproduction, the F1 testes were examined for changes in the expression of steroidogenesis- and spermatogenesis- related genes. RT-PCR analysis demonstrated that MXC significantly decreased Cyp11a1 and increased Cyp19a1; furthermore, it downregulated certain spermatogenic genes (Dazl, Boll, Rarg, Stra8 and Cyclin-a1). In summary, perinatal exposure to low-dose MXC disturbs the testicular development in mice. This animal study of exposure to low-dose MXC in F1 males suggests similar dysfunctional effects on male reproduction in humans.
format	article
author	Xiaohong Du Hua Zhang Yuanwu Liu Wanpeng Yu Chaobin Huang Xiangdong Li
author_facet	Xiaohong Du Hua Zhang Yuanwu Liu Wanpeng Yu Chaobin Huang Xiangdong Li
author_sort	Xiaohong Du
title	Perinatal exposure to low-dose methoxychlor impairs testicular development in C57BL/6 mice.
title_short	Perinatal exposure to low-dose methoxychlor impairs testicular development in C57BL/6 mice.
title_full	Perinatal exposure to low-dose methoxychlor impairs testicular development in C57BL/6 mice.
title_fullStr	Perinatal exposure to low-dose methoxychlor impairs testicular development in C57BL/6 mice.
title_full_unstemmed	Perinatal exposure to low-dose methoxychlor impairs testicular development in C57BL/6 mice.
title_sort	perinatal exposure to low-dose methoxychlor impairs testicular development in c57bl/6 mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/90b0d9c0b04b4260b06a8992b9a65eb9
work_keys_str_mv	AT xiaohongdu perinatalexposuretolowdosemethoxychlorimpairstesticulardevelopmentinc57bl6mice AT huazhang perinatalexposuretolowdosemethoxychlorimpairstesticulardevelopmentinc57bl6mice AT yuanwuliu perinatalexposuretolowdosemethoxychlorimpairstesticulardevelopmentinc57bl6mice AT wanpengyu perinatalexposuretolowdosemethoxychlorimpairstesticulardevelopmentinc57bl6mice AT chaobinhuang perinatalexposuretolowdosemethoxychlorimpairstesticulardevelopmentinc57bl6mice AT xiangdongli perinatalexposuretolowdosemethoxychlorimpairstesticulardevelopmentinc57bl6mice
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Perinatal exposure to low-dose methoxychlor impairs testicular development in C57BL/6 mice.

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