Heat shock transcription factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle.
Hypertrophic stimuli, such as mechanical stress and overloading, induce stress response, which is mediated by heat shock transcription factor 1 (HSF1), and up-regulate heat shock proteins (HSPs) in mammalian skeletal muscles. Therefore, HSF1-associated stress response may play a key role in loading-...
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2013
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oai:doaj.org-article:90b32974e9c94f95ac18ac62a1c1899d2021-11-18T08:49:59ZHeat shock transcription factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle.1932-620310.1371/journal.pone.0077788https://doaj.org/article/90b32974e9c94f95ac18ac62a1c1899d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24167582/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Hypertrophic stimuli, such as mechanical stress and overloading, induce stress response, which is mediated by heat shock transcription factor 1 (HSF1), and up-regulate heat shock proteins (HSPs) in mammalian skeletal muscles. Therefore, HSF1-associated stress response may play a key role in loading-associated skeletal muscle hypertrophy. The purpose of this study was to investigate the effects of HSF1-deficiency on skeletal muscle hypertrophy caused by overloading. Functional overloading on the left soleus was performed by cutting the distal tendons of gastrocnemius and plantaris muscles for 4 weeks. The right muscle served as the control. Soleus muscles from both hindlimbs were dissected 2 and 4 weeks after the operation. Hypertrophy of soleus muscle in HSF1-null mice was partially inhibited, compared with that in wild-type (C57BL/6J) mice. Absence of HSF1 partially attenuated the increase of muscle wet weight and fiber cross-sectional area of overloaded soleus muscle. Population of Pax7-positive muscle satellite cells in HSF1-null mice was significantly less than that in wild-type mice following 2 weeks of overloading (p<0.05). Significant up-regulations of interleukin (IL)-1β and tumor necrosis factor mRNAs were observed in HSF1-null, but not in wild-type, mice following 2 weeks of overloading. Overloading-related increases of IL-6 and AFT3 mRNA expressions seen after 2 weeks of overloading tended to decrease after 4 weeks in both types of mice. In HSF1-null mice, however, the significant overloading-related increase in the expression of IL-6, not ATF3, mRNA was noted even at 4th week. Inhibition of muscle hypertrophy might be attributed to the greater and prolonged enhancement of IL-6 expression. HSF1 and/or HSF1-mediated stress response may, in part, play a key role in loading-induced skeletal muscle hypertrophy.Tomoyuki KoyaSono NishizawaYoshitaka OhnoAyumi GotoAkihiro IkutaMiho SuzukiTomotaka OhiraTatsuro EgawaAkira NakaiTakao SugiuraYoshinobu OhiraToshitada YoshiokaMoroe BeppuKatsumasa GotoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e77788 (2013) |
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Medicine R Science Q Tomoyuki Koya Sono Nishizawa Yoshitaka Ohno Ayumi Goto Akihiro Ikuta Miho Suzuki Tomotaka Ohira Tatsuro Egawa Akira Nakai Takao Sugiura Yoshinobu Ohira Toshitada Yoshioka Moroe Beppu Katsumasa Goto Heat shock transcription factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle. |
| description |
Hypertrophic stimuli, such as mechanical stress and overloading, induce stress response, which is mediated by heat shock transcription factor 1 (HSF1), and up-regulate heat shock proteins (HSPs) in mammalian skeletal muscles. Therefore, HSF1-associated stress response may play a key role in loading-associated skeletal muscle hypertrophy. The purpose of this study was to investigate the effects of HSF1-deficiency on skeletal muscle hypertrophy caused by overloading. Functional overloading on the left soleus was performed by cutting the distal tendons of gastrocnemius and plantaris muscles for 4 weeks. The right muscle served as the control. Soleus muscles from both hindlimbs were dissected 2 and 4 weeks after the operation. Hypertrophy of soleus muscle in HSF1-null mice was partially inhibited, compared with that in wild-type (C57BL/6J) mice. Absence of HSF1 partially attenuated the increase of muscle wet weight and fiber cross-sectional area of overloaded soleus muscle. Population of Pax7-positive muscle satellite cells in HSF1-null mice was significantly less than that in wild-type mice following 2 weeks of overloading (p<0.05). Significant up-regulations of interleukin (IL)-1β and tumor necrosis factor mRNAs were observed in HSF1-null, but not in wild-type, mice following 2 weeks of overloading. Overloading-related increases of IL-6 and AFT3 mRNA expressions seen after 2 weeks of overloading tended to decrease after 4 weeks in both types of mice. In HSF1-null mice, however, the significant overloading-related increase in the expression of IL-6, not ATF3, mRNA was noted even at 4th week. Inhibition of muscle hypertrophy might be attributed to the greater and prolonged enhancement of IL-6 expression. HSF1 and/or HSF1-mediated stress response may, in part, play a key role in loading-induced skeletal muscle hypertrophy. |
| format |
article |
| author |
Tomoyuki Koya Sono Nishizawa Yoshitaka Ohno Ayumi Goto Akihiro Ikuta Miho Suzuki Tomotaka Ohira Tatsuro Egawa Akira Nakai Takao Sugiura Yoshinobu Ohira Toshitada Yoshioka Moroe Beppu Katsumasa Goto |
| author_facet |
Tomoyuki Koya Sono Nishizawa Yoshitaka Ohno Ayumi Goto Akihiro Ikuta Miho Suzuki Tomotaka Ohira Tatsuro Egawa Akira Nakai Takao Sugiura Yoshinobu Ohira Toshitada Yoshioka Moroe Beppu Katsumasa Goto |
| author_sort |
Tomoyuki Koya |
| title |
Heat shock transcription factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle. |
| title_short |
Heat shock transcription factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle. |
| title_full |
Heat shock transcription factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle. |
| title_fullStr |
Heat shock transcription factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle. |
| title_full_unstemmed |
Heat shock transcription factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle. |
| title_sort |
heat shock transcription factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/90b32974e9c94f95ac18ac62a1c1899d |
| work_keys_str_mv |
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