Responses of denitrifying bacterial communities to short-term waterlogging of soils

Abstract Agricultural soil is often subjected to waterlogging after heavy rainfalls, resulting in sharp and explosive increases in the emission of nitrous oxide (N2O), an important greenhouse gas primarily released from agricultural soil ecosystems. Previous studies on waterlogged soil examined the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yong Wang, Yoshitaka Uchida, Yumi Shimomura, Hiroko Akiyama, Masahito Hayatsu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/90b7413d215d4c198fcec0b96014a043
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:90b7413d215d4c198fcec0b96014a043
record_format	dspace
spelling	oai:doaj.org-article:90b7413d215d4c198fcec0b96014a0432021-12-02T12:32:53ZResponses of denitrifying bacterial communities to short-term waterlogging of soils10.1038/s41598-017-00953-82045-2322https://doaj.org/article/90b7413d215d4c198fcec0b96014a0432017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00953-8https://doaj.org/toc/2045-2322Abstract Agricultural soil is often subjected to waterlogging after heavy rainfalls, resulting in sharp and explosive increases in the emission of nitrous oxide (N2O), an important greenhouse gas primarily released from agricultural soil ecosystems. Previous studies on waterlogged soil examined the abundance of denitrifiers but not the composition of denitrifier communities in soil. Also, the PCR primers used in those studies could only detect partial groups of denitrifiers. Here, we performed pyrosequencing analyses with the aid of recently developed PCR primers exhibiting high coverage for three denitrification genes, nirK, nirS, and nosZ to examine the effect of short-term waterlogging on denitrifier communities in soil. We found that microbial communities harboring denitrification genes in the top 5 cm of soil distributed according to soil depth, water-soluble carbon, and nitrate nitrogen. Short-term waterlogging scarcely affected abundance, richness, or the alpha-diversities of microbial communities harboring nirK, nirS, and nosZ genes, but significantly affected their composition, particularly in microbial communities at soil depths of 0 to 1 cm. Our results indicated that the composition of denitrifying microbial communities but not the abundance of denitrifiers in soil was responsive to short-term waterlogging of an agricultural soil ecosystem.Yong WangYoshitaka UchidaYumi ShimomuraHiroko AkiyamaMasahito HayatsuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yong Wang Yoshitaka Uchida Yumi Shimomura Hiroko Akiyama Masahito Hayatsu Responses of denitrifying bacterial communities to short-term waterlogging of soils
description	Abstract Agricultural soil is often subjected to waterlogging after heavy rainfalls, resulting in sharp and explosive increases in the emission of nitrous oxide (N2O), an important greenhouse gas primarily released from agricultural soil ecosystems. Previous studies on waterlogged soil examined the abundance of denitrifiers but not the composition of denitrifier communities in soil. Also, the PCR primers used in those studies could only detect partial groups of denitrifiers. Here, we performed pyrosequencing analyses with the aid of recently developed PCR primers exhibiting high coverage for three denitrification genes, nirK, nirS, and nosZ to examine the effect of short-term waterlogging on denitrifier communities in soil. We found that microbial communities harboring denitrification genes in the top 5 cm of soil distributed according to soil depth, water-soluble carbon, and nitrate nitrogen. Short-term waterlogging scarcely affected abundance, richness, or the alpha-diversities of microbial communities harboring nirK, nirS, and nosZ genes, but significantly affected their composition, particularly in microbial communities at soil depths of 0 to 1 cm. Our results indicated that the composition of denitrifying microbial communities but not the abundance of denitrifiers in soil was responsive to short-term waterlogging of an agricultural soil ecosystem.
format	article
author	Yong Wang Yoshitaka Uchida Yumi Shimomura Hiroko Akiyama Masahito Hayatsu
author_facet	Yong Wang Yoshitaka Uchida Yumi Shimomura Hiroko Akiyama Masahito Hayatsu
author_sort	Yong Wang
title	Responses of denitrifying bacterial communities to short-term waterlogging of soils
title_short	Responses of denitrifying bacterial communities to short-term waterlogging of soils
title_full	Responses of denitrifying bacterial communities to short-term waterlogging of soils
title_fullStr	Responses of denitrifying bacterial communities to short-term waterlogging of soils
title_full_unstemmed	Responses of denitrifying bacterial communities to short-term waterlogging of soils
title_sort	responses of denitrifying bacterial communities to short-term waterlogging of soils
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/90b7413d215d4c198fcec0b96014a043
work_keys_str_mv	AT yongwang responsesofdenitrifyingbacterialcommunitiestoshorttermwaterloggingofsoils AT yoshitakauchida responsesofdenitrifyingbacterialcommunitiestoshorttermwaterloggingofsoils AT yumishimomura responsesofdenitrifyingbacterialcommunitiestoshorttermwaterloggingofsoils AT hirokoakiyama responsesofdenitrifyingbacterialcommunitiestoshorttermwaterloggingofsoils AT masahitohayatsu responsesofdenitrifyingbacterialcommunitiestoshorttermwaterloggingofsoils
_version_	1718393933526990848

Responses of denitrifying bacterial communities to short-term waterlogging of soils

Ejemplares similares