Genogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d

Fufa Dawo Bari Department of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, EthiopiaCorrespondence: Fufa Dawo BariDepartment of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medic...

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Autor principal:	Bari FD
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	infectious bursal disease virus vp2 gene sequence geno-groups sub geno-groups virulent strains Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/90b78183c8b44958950fabc90098b701
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spelling	oai:doaj.org-article:90b78183c8b44958950fabc90098b7012021-12-02T14:29:47ZGenogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d2230-2034https://doaj.org/article/90b78183c8b44958950fabc90098b7012021-02-01T00:00:00Zhttps://www.dovepress.com/genogrouping-of-infectious-bursal-disease-viruses-circulating-in-ethio-peer-reviewed-article-VMRRhttps://doaj.org/toc/2230-2034Fufa Dawo Bari Department of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, EthiopiaCorrespondence: Fufa Dawo BariDepartment of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medicine and Agriculture, Addis Ababa University, P O Box 34, Bishoftu, EthiopiaTel +251 929190312Fax +251 114339933Email fufa.dawo@aau.edu.etIntroduction: In 2017 infectious bursal disease viruses (IBDVs) were reclassified into genogroups based on nature of clustering on a phylogenetic tree constructed using VP2 gene sequence data rather than according to their pathotype and/or antigenic types. Ethiopian IBD viruses were not reclassified according to the proposed genogrouping.Methods: In order to genogroup the Ethiopian IBDVs, available VP2 gene sequences data together with reference strain sequences were retrieved from GenBank and genogrouped as recently recommended based on evolutionary tree reconstruction and determination of their clustering on the phylogenetic tree.Results: The Ethiopian IBDVs were grouped into genogroups 1 and 3 that antigenically represent classically virulent and very virulent IBDVs, respectively. The genogroup 1 IBDVs were clustered with the vaccine strain while the genogroup 3 viruses were clustered with four known viruses belonging to sub-genogroup 3a and sub-genogroup 3b. Almost half of the Ethiopian IBDVs reported did not cluster with the specific sub-groups of genogroup 3; rather, the isolates were clustered differently suggesting they deserve a different sub-genogroup tentatively proposed as 3d. The two genogroups observed based on clustering on a phylogenetic tree were supported by corresponding deduced amino acid changes in similar positions in VP2 sequences. In addition, virulence marker amino acid genes coupled with second major hydrophilic region (amino acid positions 314– 325) were predicted in these sequences that could be responsible for the occurrence of IBD outbreaks.Conclusion: A new sub-genogroup of IBDVs, 3d, were observed in the sequences that could be one of the reasons for the frequent occurrence of IBD outbreaks and questions the protective potential of the existing vaccine. To institute disease control in the country, the effectiveness of the vaccine in use needs to be assessed in vivo against both genogroups 1 and 3 viruses and all three sub-genogroup 3 viruses circulating in the country.Keywords: infectious bursal disease virus, VP2 gene sequence, genogroups, sub-genogroups, virulent strainsBari FDDove Medical Pressarticleinfectious bursal disease virusvp2 gene sequencegeno-groupssub geno-groupsvirulent strainsVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol Volume 12, Pp 43-52 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	infectious bursal disease virus vp2 gene sequence geno-groups sub geno-groups virulent strains Veterinary medicine SF600-1100
spellingShingle	infectious bursal disease virus vp2 gene sequence geno-groups sub geno-groups virulent strains Veterinary medicine SF600-1100 Bari FD Genogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d
description	Fufa Dawo Bari Department of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, EthiopiaCorrespondence: Fufa Dawo BariDepartment of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medicine and Agriculture, Addis Ababa University, P O Box 34, Bishoftu, EthiopiaTel +251 929190312Fax +251 114339933Email fufa.dawo@aau.edu.etIntroduction: In 2017 infectious bursal disease viruses (IBDVs) were reclassified into genogroups based on nature of clustering on a phylogenetic tree constructed using VP2 gene sequence data rather than according to their pathotype and/or antigenic types. Ethiopian IBD viruses were not reclassified according to the proposed genogrouping.Methods: In order to genogroup the Ethiopian IBDVs, available VP2 gene sequences data together with reference strain sequences were retrieved from GenBank and genogrouped as recently recommended based on evolutionary tree reconstruction and determination of their clustering on the phylogenetic tree.Results: The Ethiopian IBDVs were grouped into genogroups 1 and 3 that antigenically represent classically virulent and very virulent IBDVs, respectively. The genogroup 1 IBDVs were clustered with the vaccine strain while the genogroup 3 viruses were clustered with four known viruses belonging to sub-genogroup 3a and sub-genogroup 3b. Almost half of the Ethiopian IBDVs reported did not cluster with the specific sub-groups of genogroup 3; rather, the isolates were clustered differently suggesting they deserve a different sub-genogroup tentatively proposed as 3d. The two genogroups observed based on clustering on a phylogenetic tree were supported by corresponding deduced amino acid changes in similar positions in VP2 sequences. In addition, virulence marker amino acid genes coupled with second major hydrophilic region (amino acid positions 314– 325) were predicted in these sequences that could be responsible for the occurrence of IBD outbreaks.Conclusion: A new sub-genogroup of IBDVs, 3d, were observed in the sequences that could be one of the reasons for the frequent occurrence of IBD outbreaks and questions the protective potential of the existing vaccine. To institute disease control in the country, the effectiveness of the vaccine in use needs to be assessed in vivo against both genogroups 1 and 3 viruses and all three sub-genogroup 3 viruses circulating in the country.Keywords: infectious bursal disease virus, VP2 gene sequence, genogroups, sub-genogroups, virulent strains
format	article
author	Bari FD
author_facet	Bari FD
author_sort	Bari FD
title	Genogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d
title_short	Genogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d
title_full	Genogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d
title_fullStr	Genogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d
title_full_unstemmed	Genogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d
title_sort	genogrouping of infectious bursal disease viruses circulating in ethiopian chickens: proposal for assigning very virulent strains in the country into new sub genogroup 3d
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/90b78183c8b44958950fabc90098b701
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Genogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d

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