Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program.

It is generally believed that the last eukaryotic common ancestor (LECA) was a unicellular organism with motile cilia. In the vertebrates, the winged-helix transcription factor FoxJ1 functions as the master regulator of motile cilia biogenesis. Despite the antiquity of cilia, their highly conserved...

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Autores principales:	Shubha Vij, Jochen C Rink, Hao Kee Ho, Deepak Babu, Michael Eitel, Vijayashankaranarayanan Narasimhan, Varnesh Tiku, Jody Westbrook, Bernd Schierwater, Sudipto Roy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/90c04b3620684d5090d575e0ef456a95
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spelling	oai:doaj.org-article:90c04b3620684d5090d575e0ef456a952021-11-18T06:20:35ZEvolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program.1553-73901553-740410.1371/journal.pgen.1003019https://doaj.org/article/90c04b3620684d5090d575e0ef456a952012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144623/pdf/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404It is generally believed that the last eukaryotic common ancestor (LECA) was a unicellular organism with motile cilia. In the vertebrates, the winged-helix transcription factor FoxJ1 functions as the master regulator of motile cilia biogenesis. Despite the antiquity of cilia, their highly conserved structure, and their mechanism of motility, the evolution of the transcriptional program controlling ciliogenesis has remained incompletely understood. In particular, it is presently not known how the generation of motile cilia is programmed outside of the vertebrates, and whether and to what extent the FoxJ1-dependent regulation is conserved. We have performed a survey of numerous eukaryotic genomes and discovered that genes homologous to foxJ1 are restricted only to organisms belonging to the unikont lineage. Using a mis-expression assay, we then obtained evidence of a conserved ability of FoxJ1 proteins from a number of diverse phyletic groups to activate the expression of a host of motile ciliary genes in zebrafish embryos. Conversely, we found that inactivation of a foxJ1 gene in Schmidtea mediterranea, a platyhelminth (flatworm) that utilizes motile cilia for locomotion, led to a profound disruption in the differentiation of motile cilia. Together, all of these findings provide the first evolutionary perspective into the transcriptional control of motile ciliogenesis and allow us to propose a conserved FoxJ1-regulated mechanism for motile cilia biogenesis back to the origin of the metazoans.Shubha VijJochen C RinkHao Kee HoDeepak BabuMichael EitelVijayashankaranarayanan NarasimhanVarnesh TikuJody WestbrookBernd SchierwaterSudipto RoyPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 8, Iss 11, p e1003019 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Shubha Vij Jochen C Rink Hao Kee Ho Deepak Babu Michael Eitel Vijayashankaranarayanan Narasimhan Varnesh Tiku Jody Westbrook Bernd Schierwater Sudipto Roy Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program.
description	It is generally believed that the last eukaryotic common ancestor (LECA) was a unicellular organism with motile cilia. In the vertebrates, the winged-helix transcription factor FoxJ1 functions as the master regulator of motile cilia biogenesis. Despite the antiquity of cilia, their highly conserved structure, and their mechanism of motility, the evolution of the transcriptional program controlling ciliogenesis has remained incompletely understood. In particular, it is presently not known how the generation of motile cilia is programmed outside of the vertebrates, and whether and to what extent the FoxJ1-dependent regulation is conserved. We have performed a survey of numerous eukaryotic genomes and discovered that genes homologous to foxJ1 are restricted only to organisms belonging to the unikont lineage. Using a mis-expression assay, we then obtained evidence of a conserved ability of FoxJ1 proteins from a number of diverse phyletic groups to activate the expression of a host of motile ciliary genes in zebrafish embryos. Conversely, we found that inactivation of a foxJ1 gene in Schmidtea mediterranea, a platyhelminth (flatworm) that utilizes motile cilia for locomotion, led to a profound disruption in the differentiation of motile cilia. Together, all of these findings provide the first evolutionary perspective into the transcriptional control of motile ciliogenesis and allow us to propose a conserved FoxJ1-regulated mechanism for motile cilia biogenesis back to the origin of the metazoans.
format	article
author	Shubha Vij Jochen C Rink Hao Kee Ho Deepak Babu Michael Eitel Vijayashankaranarayanan Narasimhan Varnesh Tiku Jody Westbrook Bernd Schierwater Sudipto Roy
author_facet	Shubha Vij Jochen C Rink Hao Kee Ho Deepak Babu Michael Eitel Vijayashankaranarayanan Narasimhan Varnesh Tiku Jody Westbrook Bernd Schierwater Sudipto Roy
author_sort	Shubha Vij
title	Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program.
title_short	Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program.
title_full	Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program.
title_fullStr	Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program.
title_full_unstemmed	Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program.
title_sort	evolutionarily ancient association of the foxj1 transcription factor with the motile ciliogenic program.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/90c04b3620684d5090d575e0ef456a95
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Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program.

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