Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens

Abstract Correlative microscopy and block-face imaging (CoMBI), a method that we previously developed, is characterized by the ability to correlate between serial block-face images as 3-dimensional (3D) datasets and sections as 2-dimensional (2D) microscopic images. CoMBI has been performed for the...

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Autores principales:	Nobukazu Ishii, Yuki Tajika, Tohru Murakami, Josephine Galipon, Hiroyoshi Shirahata, Ryo Mukai, Daisuke Uehara, Ryosuke Kaneko, Yuichi Yamazaki, Yuhei Yoshimoto, Hirohide Iwasaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/90c1964eebc94adfb095779045a57f50
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spelling	oai:doaj.org-article:90c1964eebc94adfb095779045a57f502021-12-02T17:45:02ZCorrelative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens10.1038/s41598-021-92485-52045-2322https://doaj.org/article/90c1964eebc94adfb095779045a57f502021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92485-5https://doaj.org/toc/2045-2322Abstract Correlative microscopy and block-face imaging (CoMBI), a method that we previously developed, is characterized by the ability to correlate between serial block-face images as 3-dimensional (3D) datasets and sections as 2-dimensional (2D) microscopic images. CoMBI has been performed for the morphological analyses of various biological specimens, and its use is expanding. However, the conventional CoMBI system utilizes a cryostat, which limits its compatibility to only frozen blocks and the resolution of the block-face image. We developed a new CoMBI system that can be applied to not only frozen blocks but also paraffin blocks, and it has an improved magnification for block-face imaging. The new system, called CoMBI-S, comprises sliding-type sectioning devices and imaging devices, and it conducts block slicing and block-face imaging automatically. Sections can also be collected and processed for microscopy as required. We also developed sample preparation methods for improving the qualities of the block-face images and 3D rendered volumes. We successfully obtained correlative 3D datasets and 2D microscopic images of zebrafish, mice, and fruit flies, which were paraffin-embedded or frozen. In addition, the 3D datasets at the highest magnification could depict a single neuron and bile canaliculus.Nobukazu IshiiYuki TajikaTohru MurakamiJosephine GaliponHiroyoshi ShirahataRyo MukaiDaisuke UeharaRyosuke KanekoYuichi YamazakiYuhei YoshimotoHirohide IwasakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nobukazu Ishii Yuki Tajika Tohru Murakami Josephine Galipon Hiroyoshi Shirahata Ryo Mukai Daisuke Uehara Ryosuke Kaneko Yuichi Yamazaki Yuhei Yoshimoto Hirohide Iwasaki Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
description	Abstract Correlative microscopy and block-face imaging (CoMBI), a method that we previously developed, is characterized by the ability to correlate between serial block-face images as 3-dimensional (3D) datasets and sections as 2-dimensional (2D) microscopic images. CoMBI has been performed for the morphological analyses of various biological specimens, and its use is expanding. However, the conventional CoMBI system utilizes a cryostat, which limits its compatibility to only frozen blocks and the resolution of the block-face image. We developed a new CoMBI system that can be applied to not only frozen blocks but also paraffin blocks, and it has an improved magnification for block-face imaging. The new system, called CoMBI-S, comprises sliding-type sectioning devices and imaging devices, and it conducts block slicing and block-face imaging automatically. Sections can also be collected and processed for microscopy as required. We also developed sample preparation methods for improving the qualities of the block-face images and 3D rendered volumes. We successfully obtained correlative 3D datasets and 2D microscopic images of zebrafish, mice, and fruit flies, which were paraffin-embedded or frozen. In addition, the 3D datasets at the highest magnification could depict a single neuron and bile canaliculus.
format	article
author	Nobukazu Ishii Yuki Tajika Tohru Murakami Josephine Galipon Hiroyoshi Shirahata Ryo Mukai Daisuke Uehara Ryosuke Kaneko Yuichi Yamazaki Yuhei Yoshimoto Hirohide Iwasaki
author_facet	Nobukazu Ishii Yuki Tajika Tohru Murakami Josephine Galipon Hiroyoshi Shirahata Ryo Mukai Daisuke Uehara Ryosuke Kaneko Yuichi Yamazaki Yuhei Yoshimoto Hirohide Iwasaki
author_sort	Nobukazu Ishii
title	Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
title_short	Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
title_full	Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
title_fullStr	Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
title_full_unstemmed	Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
title_sort	correlative microscopy and block-face imaging (combi) method for both paraffin-embedded and frozen specimens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/90c1964eebc94adfb095779045a57f50
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Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens

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