Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene

Excessive activity of cytokines, as well as their deficiency, promote a regulatory imbalance of immune system, which may cause persistent inflammation and, as a consequence, loss of pregnancy. The purpose of our work was to study the role of cytokine expression in the development of endometriosis in...

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Autores principales:	O. A. Kazakova, O. V. Dolgikh
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	cytokines interleukins hydroxybenzene reproduction endometriosis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/90c593fe228d454ab73e17eed7278eb7
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spelling	oai:doaj.org-article:90c593fe228d454ab73e17eed7278eb72021-11-18T08:03:50ZCytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene1563-06252313-741X10.15789/1563-0625-CPF-2054https://doaj.org/article/90c593fe228d454ab73e17eed7278eb72021-03-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2054https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XExcessive activity of cytokines, as well as their deficiency, promote a regulatory imbalance of immune system, which may cause persistent inflammation and, as a consequence, loss of pregnancy. The purpose of our work was to study the role of cytokine expression in the development of endometriosis in women exposed to excessive environmental contamination with hydroxybenzene. The study involved 106 women of reproductive age, divided into 4 groups according to two criteria: the presence or absence of reproductive disorders (endometriosis), as well as levels of blood contamination with hydroxybenzene compared to the reference ranges. The groups of women were comparable in age, ethnicity, and social status. The level of phenol in blood was measured by gas chromatography, and the cytokine levels were determined by enzyme immunoassay. The use of parametric and nonparametric statistical criteria as well as adjustments for multiple Bonferoni comparisons, allowed us to establish significant differences between the groups, according to the levels of IL-1β, IL-8, IL-10. The expression of interleukin 8 (IL-8) in women with endometriosis was found to be significantly higher than in healthy ones. At the same time, the observed features of IL-8, interleukin 1β (IL-1β) expression correlate with the overproduction of interleukin 10 (IL-10) associated with excessive contamination of bio-environments with hydroxybenzene. The results of using non-parametric correlation analysis of the Spearman data revealed a positive correlation between IL-10 production and hydroxybenzene levels in the blood, as well as inverse relationship between IL-10 and IL-8 expression. Thus, evidence has been obtained of the involvement of exogenous estrogen hydroxybenzene in development of reproductive disorders, probably, formed under active participation of increased anti-inflammatory IL-10 cytokine, which, by antagonism to proinflammatory mediators (e.g., IL-8), seems to promote apparent transition from acute phase of endometriosis to chronic disorder, thus reducing the reproductive potential of women. It is known that IL-8 expression significantly correlates with development of endometriosis, but excessive phenol exposure may increase the anti-inflammatory IL-10 expression, thereby suppressing the activity of IL-8. Suppressed activity of proinflammatory cytokines by phenol may lead to chronic inflammation and impaired reproductive functions.O. A. KazakovaO. V. DolgikhSPb RAACIarticlecytokinesinterleukinshydroxybenzenereproductionendometriosisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 1, Pp 173-178 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	cytokines interleukins hydroxybenzene reproduction endometriosis Immunologic diseases. Allergy RC581-607
spellingShingle	cytokines interleukins hydroxybenzene reproduction endometriosis Immunologic diseases. Allergy RC581-607 O. A. Kazakova O. V. Dolgikh Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene
description	Excessive activity of cytokines, as well as their deficiency, promote a regulatory imbalance of immune system, which may cause persistent inflammation and, as a consequence, loss of pregnancy. The purpose of our work was to study the role of cytokine expression in the development of endometriosis in women exposed to excessive environmental contamination with hydroxybenzene. The study involved 106 women of reproductive age, divided into 4 groups according to two criteria: the presence or absence of reproductive disorders (endometriosis), as well as levels of blood contamination with hydroxybenzene compared to the reference ranges. The groups of women were comparable in age, ethnicity, and social status. The level of phenol in blood was measured by gas chromatography, and the cytokine levels were determined by enzyme immunoassay. The use of parametric and nonparametric statistical criteria as well as adjustments for multiple Bonferoni comparisons, allowed us to establish significant differences between the groups, according to the levels of IL-1β, IL-8, IL-10. The expression of interleukin 8 (IL-8) in women with endometriosis was found to be significantly higher than in healthy ones. At the same time, the observed features of IL-8, interleukin 1β (IL-1β) expression correlate with the overproduction of interleukin 10 (IL-10) associated with excessive contamination of bio-environments with hydroxybenzene. The results of using non-parametric correlation analysis of the Spearman data revealed a positive correlation between IL-10 production and hydroxybenzene levels in the blood, as well as inverse relationship between IL-10 and IL-8 expression. Thus, evidence has been obtained of the involvement of exogenous estrogen hydroxybenzene in development of reproductive disorders, probably, formed under active participation of increased anti-inflammatory IL-10 cytokine, which, by antagonism to proinflammatory mediators (e.g., IL-8), seems to promote apparent transition from acute phase of endometriosis to chronic disorder, thus reducing the reproductive potential of women. It is known that IL-8 expression significantly correlates with development of endometriosis, but excessive phenol exposure may increase the anti-inflammatory IL-10 expression, thereby suppressing the activity of IL-8. Suppressed activity of proinflammatory cytokines by phenol may lead to chronic inflammation and impaired reproductive functions.
format	article
author	O. A. Kazakova O. V. Dolgikh
author_facet	O. A. Kazakova O. V. Dolgikh
author_sort	O. A. Kazakova
title	Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene
title_short	Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene
title_full	Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene
title_fullStr	Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene
title_full_unstemmed	Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene
title_sort	cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/90c593fe228d454ab73e17eed7278eb7
work_keys_str_mv	AT oakazakova cytokineprofilefeaturesinwomenwithreproductivedisordersexposedtoexcessiveenvironmentalcontaminationwithhydroxybenzene AT ovdolgikh cytokineprofilefeaturesinwomenwithreproductivedisordersexposedtoexcessiveenvironmentalcontaminationwithhydroxybenzene
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Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene

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