Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense.
A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal-...
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2014
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oai:doaj.org-article:90c75538544d4575aeff4ee04206414b2021-11-25T06:08:51ZStructure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense.1932-620310.1371/journal.pone.0100380https://doaj.org/article/90c75538544d4575aeff4ee04206414b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25014571/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.Xiao-Qiang HanGar-Lee YueRui-Qi YueCai-Xia DongChung-Lap ChanChun-Hay KoWing-Shing CheungKe-Wang LuoHui DaiChun-Kwok WongPing-Chung LeungQuan-Bin HanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e100380 (2014) |
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Medicine R Science Q Xiao-Qiang Han Gar-Lee Yue Rui-Qi Yue Cai-Xia Dong Chung-Lap Chan Chun-Hay Ko Wing-Shing Cheung Ke-Wang Luo Hui Dai Chun-Kwok Wong Ping-Chung Leung Quan-Bin Han Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| description |
A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells. |
| format |
article |
| author |
Xiao-Qiang Han Gar-Lee Yue Rui-Qi Yue Cai-Xia Dong Chung-Lap Chan Chun-Hay Ko Wing-Shing Cheung Ke-Wang Luo Hui Dai Chun-Kwok Wong Ping-Chung Leung Quan-Bin Han |
| author_facet |
Xiao-Qiang Han Gar-Lee Yue Rui-Qi Yue Cai-Xia Dong Chung-Lap Chan Chun-Hay Ko Wing-Shing Cheung Ke-Wang Luo Hui Dai Chun-Kwok Wong Ping-Chung Leung Quan-Bin Han |
| author_sort |
Xiao-Qiang Han |
| title |
Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_short |
Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_full |
Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_fullStr |
Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_full_unstemmed |
Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_sort |
structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of ganoderma sinense. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/90c75538544d4575aeff4ee04206414b |
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