A novel paper MAP method for rapid high resolution histological analysis

Abstract Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-cle...

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Autores principales:	Mirae Lee, Jiwon Woo, Doh-Hee Kim, Yu-Mi Yang, Eunice Yoojin Lee, Jung-Hee Kim, Seok-Gu Kang, Jin-Kyung Shim, Jeong-Yoon Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/90d7f1a0de4d40a28c4ee4a10f9ba55a
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spelling	oai:doaj.org-article:90d7f1a0de4d40a28c4ee4a10f9ba55a2021-12-05T12:12:28ZA novel paper MAP method for rapid high resolution histological analysis10.1038/s41598-021-02632-12045-2322https://doaj.org/article/90d7f1a0de4d40a28c4ee4a10f9ba55a2021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02632-1https://doaj.org/toc/2045-2322Abstract Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-clearing technology, such as Magnified Analysis of Proteome (MAP), have significantly improved our ability to study biological-structures in three-dimensional space; however, their wide applicability to a variety of tissues is limited by long incubation-times and a need for advanced imaging-systems that are not readily available in most-laboratories. Here, we present optimized MAP-based method for paper-thin samples, Paper-MAP, which allow for rapid clearing and subsequent imaging of three-dimensional sections derived from various tissues using conventional confocal-microscopy. Paper-MAP successfully clear tissues within 1-day, compared to the original-MAP, without significant differences in achieved optical-transparency. As a proof-of-concept, we investigated the vasculature and neuronal-networks of a variety of human and rodent tissues processed via Paper-MAP, in both healthy and diseased contexts, including Alzheimer’s disease and glioma.Mirae LeeJiwon WooDoh-Hee KimYu-Mi YangEunice Yoojin LeeJung-Hee KimSeok-Gu KangJin-Kyung ShimJeong-Yoon ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mirae Lee Jiwon Woo Doh-Hee Kim Yu-Mi Yang Eunice Yoojin Lee Jung-Hee Kim Seok-Gu Kang Jin-Kyung Shim Jeong-Yoon Park A novel paper MAP method for rapid high resolution histological analysis
description	Abstract Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-clearing technology, such as Magnified Analysis of Proteome (MAP), have significantly improved our ability to study biological-structures in three-dimensional space; however, their wide applicability to a variety of tissues is limited by long incubation-times and a need for advanced imaging-systems that are not readily available in most-laboratories. Here, we present optimized MAP-based method for paper-thin samples, Paper-MAP, which allow for rapid clearing and subsequent imaging of three-dimensional sections derived from various tissues using conventional confocal-microscopy. Paper-MAP successfully clear tissues within 1-day, compared to the original-MAP, without significant differences in achieved optical-transparency. As a proof-of-concept, we investigated the vasculature and neuronal-networks of a variety of human and rodent tissues processed via Paper-MAP, in both healthy and diseased contexts, including Alzheimer’s disease and glioma.
format	article
author	Mirae Lee Jiwon Woo Doh-Hee Kim Yu-Mi Yang Eunice Yoojin Lee Jung-Hee Kim Seok-Gu Kang Jin-Kyung Shim Jeong-Yoon Park
author_facet	Mirae Lee Jiwon Woo Doh-Hee Kim Yu-Mi Yang Eunice Yoojin Lee Jung-Hee Kim Seok-Gu Kang Jin-Kyung Shim Jeong-Yoon Park
author_sort	Mirae Lee
title	A novel paper MAP method for rapid high resolution histological analysis
title_short	A novel paper MAP method for rapid high resolution histological analysis
title_full	A novel paper MAP method for rapid high resolution histological analysis
title_fullStr	A novel paper MAP method for rapid high resolution histological analysis
title_full_unstemmed	A novel paper MAP method for rapid high resolution histological analysis
title_sort	novel paper map method for rapid high resolution histological analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/90d7f1a0de4d40a28c4ee4a10f9ba55a
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A novel paper MAP method for rapid high resolution histological analysis

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