Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats

Bin-Salman SA, Amasha RH, Jastaniah SD, Aly MM, Altaif K. 2018. Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats. Biodiversitas 19: 1828-1834. Saline habitats, like the Dead Sea, are unusual extreme environ...

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Autores principales:	SUZAN AHMED BIN-SALMAN, REDA HASSAN AMASHA, SAMYAH D. JASTANIAH, MAGEDA M. ALY, KHALIL ALTAIF
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	16s rrna gene sequence arabian gulf dead sea extremophiles halophiles red sea Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/90db5b69e3274151bc8fff9c3d663462
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spelling	oai:doaj.org-article:90db5b69e3274151bc8fff9c3d6634622021-11-16T14:04:05ZIsolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats1412-033X2085-472210.13057/biodiv/d190533https://doaj.org/article/90db5b69e3274151bc8fff9c3d6634622018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2953https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Bin-Salman SA, Amasha RH, Jastaniah SD, Aly MM, Altaif K. 2018. Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats. Biodiversitas 19: 1828-1834. Saline habitats, like the Dead Sea, are unusual extreme environments, due to their extreme salinity. Most saline habitats originate from the evaporation of seawater, and have a nearly neutral to slightly alkaline pH (such as the Red Sea (pH8.3) and Arabian Gulf, pH8.3). Ten halophilic bacterial strains (two Gram-negative) belonging to the family of Halomonadaceae and (eight Gram-positive), belonging to the family of Bacillaceae, were isolated from the Red Sea, Arabian Gulf, and Dead Sea by subjecting the isolates to a high salinity medium, followed by identification using 16S rRNA gene sequencing. Four of isolates were designated on the basis of their tolerance to high salinity; SBR1 exhibited 97% homology to Halomonas aquamarina, SBR2 showed 97% homology to Sediminibacillus sp., (Red Sea), SBA9 exhibited 94% homology to Halobacillus sp., (Arabian Gulf) and SBD17 gave 98% homology to Halobacillus dabanensis (Dead Sea). The isolates were also characterized by their physiological parameters, SBR1 showed optimum growth at 30°C, pH8.5 and1.5M NaCl, SBR2 at 30°C, pH6.0 and 1M NaCl. Optimum conditions for SBA9 were 35°C, pH6.5 and 1M NaCl and for SBD17, 37°C, pH7.0 and 1M NaCl.SUZAN AHMED BIN-SALMANREDA HASSAN AMASHASAMYAH D. JASTANIAHMAGEDA M. ALYKHALIL ALTAIFMBI & UNS Soloarticle16s rrna gene sequencearabian gulfdead seaextremophileshalophilesred seaBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 5, Pp 1828-1834 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna gene sequence arabian gulf dead sea extremophiles halophiles red sea Biology (General) QH301-705.5
spellingShingle	16s rrna gene sequence arabian gulf dead sea extremophiles halophiles red sea Biology (General) QH301-705.5 SUZAN AHMED BIN-SALMAN REDA HASSAN AMASHA SAMYAH D. JASTANIAH MAGEDA M. ALY KHALIL ALTAIF Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats
description	Bin-Salman SA, Amasha RH, Jastaniah SD, Aly MM, Altaif K. 2018. Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats. Biodiversitas 19: 1828-1834. Saline habitats, like the Dead Sea, are unusual extreme environments, due to their extreme salinity. Most saline habitats originate from the evaporation of seawater, and have a nearly neutral to slightly alkaline pH (such as the Red Sea (pH8.3) and Arabian Gulf, pH8.3). Ten halophilic bacterial strains (two Gram-negative) belonging to the family of Halomonadaceae and (eight Gram-positive), belonging to the family of Bacillaceae, were isolated from the Red Sea, Arabian Gulf, and Dead Sea by subjecting the isolates to a high salinity medium, followed by identification using 16S rRNA gene sequencing. Four of isolates were designated on the basis of their tolerance to high salinity; SBR1 exhibited 97% homology to Halomonas aquamarina, SBR2 showed 97% homology to Sediminibacillus sp., (Red Sea), SBA9 exhibited 94% homology to Halobacillus sp., (Arabian Gulf) and SBD17 gave 98% homology to Halobacillus dabanensis (Dead Sea). The isolates were also characterized by their physiological parameters, SBR1 showed optimum growth at 30°C, pH8.5 and1.5M NaCl, SBR2 at 30°C, pH6.0 and 1M NaCl. Optimum conditions for SBA9 were 35°C, pH6.5 and 1M NaCl and for SBD17, 37°C, pH7.0 and 1M NaCl.
format	article
author	SUZAN AHMED BIN-SALMAN REDA HASSAN AMASHA SAMYAH D. JASTANIAH MAGEDA M. ALY KHALIL ALTAIF
author_facet	SUZAN AHMED BIN-SALMAN REDA HASSAN AMASHA SAMYAH D. JASTANIAH MAGEDA M. ALY KHALIL ALTAIF
author_sort	SUZAN AHMED BIN-SALMAN
title	Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats
title_short	Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats
title_full	Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats
title_fullStr	Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats
title_full_unstemmed	Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats
title_sort	isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/90db5b69e3274151bc8fff9c3d663462
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_version_	1718426436925128704

Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats

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