Opposite-view digital holographic microscopy with autofocusing capability

Abstract Digital holographic microscopy (DHM) has its intrinsic ability to refocusing a sample by numerically propagating an object wave from its hologram plane to its image plane. In this paper opposite-view digital holographic microscopy (OV-DHM) is demonstrated for autofocusing, namely, digitally...

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Autores principales:	Juanjuan Zheng, Peng Gao, Xiaopeng Shao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/90dd50571be94e7e8bf85ebe8c2bf380
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spelling	oai:doaj.org-article:90dd50571be94e7e8bf85ebe8c2bf3802021-12-02T11:51:11ZOpposite-view digital holographic microscopy with autofocusing capability10.1038/s41598-017-04568-x2045-2322https://doaj.org/article/90dd50571be94e7e8bf85ebe8c2bf3802017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04568-xhttps://doaj.org/toc/2045-2322Abstract Digital holographic microscopy (DHM) has its intrinsic ability to refocusing a sample by numerically propagating an object wave from its hologram plane to its image plane. In this paper opposite-view digital holographic microscopy (OV-DHM) is demonstrated for autofocusing, namely, digitally determining the location of the image plane, and refocusing the object wave without human intervention. In OV-DHM, a specimen is illuminated from two sides in a 4π-alike configuration, and two holograms are generated and recorded by a CCD camera along two orthogonal polarization orientations. The image plane of the sample is determined by finding the minimal variation between the two object waves, and consequently refocusing is performed by propagating the waves to the image plane. Furthermore, the field of view (FOV) of OV-DHM can be extended by combining the two object waves which have an angle in-between. The proposed technique also has the potential to reduce speckle noise and out-of-focus background.Juanjuan ZhengPeng GaoXiaopeng ShaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juanjuan Zheng Peng Gao Xiaopeng Shao Opposite-view digital holographic microscopy with autofocusing capability
description	Abstract Digital holographic microscopy (DHM) has its intrinsic ability to refocusing a sample by numerically propagating an object wave from its hologram plane to its image plane. In this paper opposite-view digital holographic microscopy (OV-DHM) is demonstrated for autofocusing, namely, digitally determining the location of the image plane, and refocusing the object wave without human intervention. In OV-DHM, a specimen is illuminated from two sides in a 4π-alike configuration, and two holograms are generated and recorded by a CCD camera along two orthogonal polarization orientations. The image plane of the sample is determined by finding the minimal variation between the two object waves, and consequently refocusing is performed by propagating the waves to the image plane. Furthermore, the field of view (FOV) of OV-DHM can be extended by combining the two object waves which have an angle in-between. The proposed technique also has the potential to reduce speckle noise and out-of-focus background.
format	article
author	Juanjuan Zheng Peng Gao Xiaopeng Shao
author_facet	Juanjuan Zheng Peng Gao Xiaopeng Shao
author_sort	Juanjuan Zheng
title	Opposite-view digital holographic microscopy with autofocusing capability
title_short	Opposite-view digital holographic microscopy with autofocusing capability
title_full	Opposite-view digital holographic microscopy with autofocusing capability
title_fullStr	Opposite-view digital holographic microscopy with autofocusing capability
title_full_unstemmed	Opposite-view digital holographic microscopy with autofocusing capability
title_sort	opposite-view digital holographic microscopy with autofocusing capability
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/90dd50571be94e7e8bf85ebe8c2bf380
work_keys_str_mv	AT juanjuanzheng oppositeviewdigitalholographicmicroscopywithautofocusingcapability AT penggao oppositeviewdigitalholographicmicroscopywithautofocusingcapability AT xiaopengshao oppositeviewdigitalholographicmicroscopywithautofocusingcapability
_version_	1718395177277587456

Opposite-view digital holographic microscopy with autofocusing capability

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