Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica

An examination of insect resistance was determined by several steps, i.e. standard sensitivity, resistance diagnosis, and determination of resistance level. Each phase was tested with feeding and residue contact methods at glass tube. Resistance ratio (RR) was determined by comparing LC50 value of f...

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Autores principales:	DANAR DONO, SYAFRI ISMAYANA, IDAR IDAR, DJOKO PRIJONO, IKHA MUSLIKHA
Formato:	article
Lenguaje:	EN ID
Publicado:	The Entomological Society of Indonesia 2015
Materias:	resistance enzyme organophosphat botanical insecticide Zoology QL1-991
Acceso en línea:	https://doaj.org/article/90ddca395a6346c18c63e60df91cb6ec
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spelling	oai:doaj.org-article:90ddca395a6346c18c63e60df91cb6ec2021-12-02T06:12:23ZStatus dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica1829-77222089-025710.5994/jei.7.1.9-27https://doaj.org/article/90ddca395a6346c18c63e60df91cb6ec2015-09-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/98https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257An examination of insect resistance was determined by several steps, i.e. standard sensitivity, resistance diagnosis, and determination of resistance level. Each phase was tested with feeding and residue contact methods at glass tube. Resistance ratio (RR) was determined by comparing LC50 value of field population with standard population. Field population of <em>C. pavonana</em> was classified resistant if it had RR 5 4. Biochemistry analysis of resistance was conducted to population of<em> C. pavonana</em> showing resistance to prophenophos insecticide. The activity analysis of acetylcholine esterase (ACHE), esterase, and Glutation Stransferase was done with spectrophotometer method. Insect which are resistant to prophenophos insecticide was tested for its sensitivity to <em>Barringtonia asiatica</em> seed extract. Result indicated that <em>C. pavonana</em> population from Pengalengan showed resistance to prophenophos synthetic insecticide. Using contact test, the highest resistance ratio value was 4.04, while by feeding assay the RR was 2.78. The study on biochemical resistance mechanisms of each field population of<em> C. pavonana</em> showed various activities of enzymatic detoxification. This could be due to the difference in the kind of insecticides exposed to each field population of <em>C. pavonana</em>. Since RR value from the contact test was higher than that of the feeding test, the resistance development of <em>C. pavonana</em> to synthetic insecticides was probably caused by physiological and biochemical changes in insect cuticle rather than the activity of detoxification enzyme. Methanolic seed extract of <em>B. asiatica</em> can be used as an alternative of resistance management of<em> C. pavonana</em> to prophenophos synthetic insecticide.DANAR DONOSYAFRI ISMAYANAIDAR IDARDJOKO PRIJONOIKHA MUSLIKHAThe Entomological Society of Indonesiaarticleresistanceenzymeorganophosphatbotanical insecticideZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 7, Iss 1, Pp 9-27 (2015)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	resistance enzyme organophosphat botanical insecticide Zoology QL1-991
spellingShingle	resistance enzyme organophosphat botanical insecticide Zoology QL1-991 DANAR DONO SYAFRI ISMAYANA IDAR IDAR DJOKO PRIJONO IKHA MUSLIKHA Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica
description	An examination of insect resistance was determined by several steps, i.e. standard sensitivity, resistance diagnosis, and determination of resistance level. Each phase was tested with feeding and residue contact methods at glass tube. Resistance ratio (RR) was determined by comparing LC50 value of field population with standard population. Field population of <em>C. pavonana</em> was classified resistant if it had RR 5 4. Biochemistry analysis of resistance was conducted to population of<em> C. pavonana</em> showing resistance to prophenophos insecticide. The activity analysis of acetylcholine esterase (ACHE), esterase, and Glutation Stransferase was done with spectrophotometer method. Insect which are resistant to prophenophos insecticide was tested for its sensitivity to <em>Barringtonia asiatica</em> seed extract. Result indicated that <em>C. pavonana</em> population from Pengalengan showed resistance to prophenophos synthetic insecticide. Using contact test, the highest resistance ratio value was 4.04, while by feeding assay the RR was 2.78. The study on biochemical resistance mechanisms of each field population of<em> C. pavonana</em> showed various activities of enzymatic detoxification. This could be due to the difference in the kind of insecticides exposed to each field population of <em>C. pavonana</em>. Since RR value from the contact test was higher than that of the feeding test, the resistance development of <em>C. pavonana</em> to synthetic insecticides was probably caused by physiological and biochemical changes in insect cuticle rather than the activity of detoxification enzyme. Methanolic seed extract of <em>B. asiatica</em> can be used as an alternative of resistance management of<em> C. pavonana</em> to prophenophos synthetic insecticide.
format	article
author	DANAR DONO SYAFRI ISMAYANA IDAR IDAR DJOKO PRIJONO IKHA MUSLIKHA
author_facet	DANAR DONO SYAFRI ISMAYANA IDAR IDAR DJOKO PRIJONO IKHA MUSLIKHA
author_sort	DANAR DONO
title	Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica
title_short	Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica
title_full	Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica
title_fullStr	Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica
title_full_unstemmed	Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica
title_sort	status dan mekanisme resistensi biokimia crocidolomia pavonana (f.) (lepidoptera: crambidae) terhadap insektisida organofosfat serta kepekaannya terhadap insektisida botani ekstrak biji barringtonia asiatica
publisher	The Entomological Society of Indonesia
publishDate	2015
url	https://doaj.org/article/90ddca395a6346c18c63e60df91cb6ec
work_keys_str_mv	AT danardono statusdanmekanismeresistensibiokimiacrocidolomiapavonanaflepidopteracrambidaeterhadapinsektisidaorganofosfatsertakepekaannyaterhadapinsektisidabotaniekstrakbijibarringtoniaasiatica AT syafriismayana statusdanmekanismeresistensibiokimiacrocidolomiapavonanaflepidopteracrambidaeterhadapinsektisidaorganofosfatsertakepekaannyaterhadapinsektisidabotaniekstrakbijibarringtoniaasiatica AT idaridar statusdanmekanismeresistensibiokimiacrocidolomiapavonanaflepidopteracrambidaeterhadapinsektisidaorganofosfatsertakepekaannyaterhadapinsektisidabotaniekstrakbijibarringtoniaasiatica AT djokoprijono statusdanmekanismeresistensibiokimiacrocidolomiapavonanaflepidopteracrambidaeterhadapinsektisidaorganofosfatsertakepekaannyaterhadapinsektisidabotaniekstrakbijibarringtoniaasiatica AT ikhamuslikha statusdanmekanismeresistensibiokimiacrocidolomiapavonanaflepidopteracrambidaeterhadapinsektisidaorganofosfatsertakepekaannyaterhadapinsektisidabotaniekstrakbijibarringtoniaasiatica
_version_	1718400002735210496

Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica

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