Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>

ABSTRACT Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. By that means, under stress, the induced MazF generates a stress-induced translation machinery (STM) composed of MazF-processed...

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Autores principales:	Akanksha Nigam, Tamar Ziv, Adi Oron-Gottesman, Hanna Engelberg-Kulka
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	bacterial stress response toxin-antitoxin proteomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/90e5b8332358454f9d3e124271316e3a
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spelling	oai:doaj.org-article:90e5b8332358454f9d3e124271316e3a2021-11-15T15:55:25ZStress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>10.1128/mBio.00340-192150-7511https://doaj.org/article/90e5b8332358454f9d3e124271316e3a2019-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00340-19https://doaj.org/toc/2150-7511ABSTRACT Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. By that means, under stress, the induced MazF generates a stress-induced translation machinery (STM) composed of MazF-processed mRNAs and selective ribosomes that specifically translate the processed mRNAs. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated through the chromosomally borne mazF gene. We show that the mRNAs of almost all of them are characterized by the presence of an ACA site up to 100 nucleotides upstream of the AUG initiator. Therefore, under stressful conditions, induced MazF processes mRNAs that are translated by STM. Furthermore, the presence of the ACA sites far upstream (up to 100 nucleotides) of the AUG initiator may still permit translation by the canonical translation machinery. Thus, such dual-translation mechanisms enable the bacterium under stress also to prepare proteins for immediate functions while coming back to normal growth conditions. IMPORTANCE The stress response, the strategy that bacteria have developed in order to cope up with all kinds of adverse conditions, is so far understood at the level of transcription. Our previous findings of a uniquely modified stress-induced translation machinery (STM) generated in E. coli under stress by the endoribonucleolytic activity of the toxin MazF opens a new chapter in understanding microbial physiology under stress at the translational level. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated by chromosomally borne MazF through STM.Akanksha NigamTamar ZivAdi Oron-GottesmanHanna Engelberg-KulkaAmerican Society for Microbiologyarticlebacterial stress responsetoxin-antitoxinproteomicsMicrobiologyQR1-502ENmBio, Vol 10, Iss 2 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial stress response toxin-antitoxin proteomics Microbiology QR1-502
spellingShingle	bacterial stress response toxin-antitoxin proteomics Microbiology QR1-502 Akanksha Nigam Tamar Ziv Adi Oron-Gottesman Hanna Engelberg-Kulka Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>
description	ABSTRACT Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. By that means, under stress, the induced MazF generates a stress-induced translation machinery (STM) composed of MazF-processed mRNAs and selective ribosomes that specifically translate the processed mRNAs. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated through the chromosomally borne mazF gene. We show that the mRNAs of almost all of them are characterized by the presence of an ACA site up to 100 nucleotides upstream of the AUG initiator. Therefore, under stressful conditions, induced MazF processes mRNAs that are translated by STM. Furthermore, the presence of the ACA sites far upstream (up to 100 nucleotides) of the AUG initiator may still permit translation by the canonical translation machinery. Thus, such dual-translation mechanisms enable the bacterium under stress also to prepare proteins for immediate functions while coming back to normal growth conditions. IMPORTANCE The stress response, the strategy that bacteria have developed in order to cope up with all kinds of adverse conditions, is so far understood at the level of transcription. Our previous findings of a uniquely modified stress-induced translation machinery (STM) generated in E. coli under stress by the endoribonucleolytic activity of the toxin MazF opens a new chapter in understanding microbial physiology under stress at the translational level. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated by chromosomally borne MazF through STM.
format	article
author	Akanksha Nigam Tamar Ziv Adi Oron-Gottesman Hanna Engelberg-Kulka
author_facet	Akanksha Nigam Tamar Ziv Adi Oron-Gottesman Hanna Engelberg-Kulka
author_sort	Akanksha Nigam
title	Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>
title_short	Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>
title_full	Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>
title_fullStr	Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>
title_full_unstemmed	Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>
title_sort	stress-induced mazf-mediated proteins in <named-content content-type="genus-species">escherichia coli</named-content>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/90e5b8332358454f9d3e124271316e3a
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Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>

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