Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea

ABSTRACT: In Korea, 4 big layer companies that possess one grandparent and 3 parent stocks are in charge of 100% of the layer chicken industry. In this study, we investigated the antimicrobial resistance of commensal 578 E. coli isolated from 20 flocks of 4-layer breeder farms (A, B, C, and D), more...

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Autores principales:	Dong gyu Kim, Koeun Kim, Sung Hyun Bae, Hye-Ri Jung, Hyo Jung Kang, Yu Jin Lee, Kwang Won Seo, Young Ju Lee
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Escherichia coli layer parent stock antimicrobial resistance Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/90f41553c0db4c24aa0aac8227d9710d
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spelling	oai:doaj.org-article:90f41553c0db4c24aa0aac8227d9710d2021-11-28T04:27:19ZComparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea0032-579110.1016/j.psj.2021.101571https://doaj.org/article/90f41553c0db4c24aa0aac8227d9710d2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005927https://doaj.org/toc/0032-5791ABSTRACT: In Korea, 4 big layer companies that possess one grandparent and 3 parent stocks are in charge of 100% of the layer chicken industry. In this study, we investigated the antimicrobial resistance of commensal 578 E. coli isolated from 20 flocks of 4-layer breeder farms (A, B, C, and D), moreover, compared the characteristics of their resistance and virulence genes. Isolates from farms B and D showed significantly higher resistance to the β-lactam antimicrobials (amoxicillin, ampicillin, and 1st-, 2nd-, and 3rd-generation cephalosporins). However, resistance to ciprofloxacin, nalidixic acid, and tetracycline was significantly higher in the isolates from farm A (P < 0.05). Interestingly, the isolates from farm C showed significantly lower resistance to most antimicrobials tested in this study. The isolates from farms B, C, and D showed the high multiple resistance to the 3 antimicrobial classes. Furthermore, the isolates from farm A showed the highest multiple resistance against the 5 classes. Among the 412 β-lactam-resistant isolates, 123 (29.9%) carried blaTEM-1, but the distribution was significantly different among the farms from 17.5% to 51.4% (P < 0.05). Similarly, the most prevalent tetracycline resistance gene in the isolates from farms B, C, and D was tetA (50.0–77.0%); however, the isolates from farm A showed the highest prevalence in tetB (70.6%). The distribution of quinolone (qnrB, qnrD, and qnrS) and sulfonamide (su12)-resistant genes were also significantly different among the farms but that of chloramphenicol (catA1)- and aminoglycoside (aac [3]-II, and aac [6′]-Ib)-resistant genes possessed no significant difference among the farms. Moreover, the isolates from farm C showed significantly higher prevalence in virulence genes (iroN, ompT, hlyF, and iss) than the other 3 farms (P < 0.05). Furthermore, the phenotypic and genotypic characteristics of E. coli isolates were significantly different among the farms, and improved management protocols are required to control of horizontal and vertical transmission of avian disease, including the dissemination of resistant bacteria in breeder flocks.Dong gyu KimKoeun KimSung Hyun BaeHye-Ri JungHyo Jung KangYu Jin LeeKwang Won SeoYoung Ju LeeElsevierarticleEscherichia colilayer parent stockantimicrobial resistanceAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101571- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli layer parent stock antimicrobial resistance Animal culture SF1-1100
spellingShingle	Escherichia coli layer parent stock antimicrobial resistance Animal culture SF1-1100 Dong gyu Kim Koeun Kim Sung Hyun Bae Hye-Ri Jung Hyo Jung Kang Yu Jin Lee Kwang Won Seo Young Ju Lee Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea
description	ABSTRACT: In Korea, 4 big layer companies that possess one grandparent and 3 parent stocks are in charge of 100% of the layer chicken industry. In this study, we investigated the antimicrobial resistance of commensal 578 E. coli isolated from 20 flocks of 4-layer breeder farms (A, B, C, and D), moreover, compared the characteristics of their resistance and virulence genes. Isolates from farms B and D showed significantly higher resistance to the β-lactam antimicrobials (amoxicillin, ampicillin, and 1st-, 2nd-, and 3rd-generation cephalosporins). However, resistance to ciprofloxacin, nalidixic acid, and tetracycline was significantly higher in the isolates from farm A (P < 0.05). Interestingly, the isolates from farm C showed significantly lower resistance to most antimicrobials tested in this study. The isolates from farms B, C, and D showed the high multiple resistance to the 3 antimicrobial classes. Furthermore, the isolates from farm A showed the highest multiple resistance against the 5 classes. Among the 412 β-lactam-resistant isolates, 123 (29.9%) carried blaTEM-1, but the distribution was significantly different among the farms from 17.5% to 51.4% (P < 0.05). Similarly, the most prevalent tetracycline resistance gene in the isolates from farms B, C, and D was tetA (50.0–77.0%); however, the isolates from farm A showed the highest prevalence in tetB (70.6%). The distribution of quinolone (qnrB, qnrD, and qnrS) and sulfonamide (su12)-resistant genes were also significantly different among the farms but that of chloramphenicol (catA1)- and aminoglycoside (aac [3]-II, and aac [6′]-Ib)-resistant genes possessed no significant difference among the farms. Moreover, the isolates from farm C showed significantly higher prevalence in virulence genes (iroN, ompT, hlyF, and iss) than the other 3 farms (P < 0.05). Furthermore, the phenotypic and genotypic characteristics of E. coli isolates were significantly different among the farms, and improved management protocols are required to control of horizontal and vertical transmission of avian disease, including the dissemination of resistant bacteria in breeder flocks.
format	article
author	Dong gyu Kim Koeun Kim Sung Hyun Bae Hye-Ri Jung Hyo Jung Kang Yu Jin Lee Kwang Won Seo Young Ju Lee
author_facet	Dong gyu Kim Koeun Kim Sung Hyun Bae Hye-Ri Jung Hyo Jung Kang Yu Jin Lee Kwang Won Seo Young Ju Lee
author_sort	Dong gyu Kim
title	Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea
title_short	Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea
title_full	Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea
title_fullStr	Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea
title_full_unstemmed	Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea
title_sort	comparison of antimicrobial resistance and molecular characterization of escherichia coli isolates from layer breeder farms in korea
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/90f41553c0db4c24aa0aac8227d9710d
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Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea

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