STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA

Abstract. The aim of present study was to determine the features of STAT6 and phospho-STAT6 (pSTAT6) expression in bronchial asthma (BA). Patients and methods. Eleven patients with allergic (atopic) steroidfree were examined, five healthy controls served as a control. Expression of proteins (STAT6 a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	V. N. Mineev, L. N. Sorokina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	bronchial asthma jak-stat system stat 6 pstat 6 lymphocytes Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/90f5e8c9404040918555d453ce63af5e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:90f5e8c9404040918555d453ce63af5e
record_format	dspace
spelling	oai:doaj.org-article:90f5e8c9404040918555d453ce63af5e2021-11-18T08:03:39ZSTAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA1563-06252313-741X10.15789/1563-0625-2007-4-5-405-410https://doaj.org/article/90f5e8c9404040918555d453ce63af5e2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/191https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The aim of present study was to determine the features of STAT6 and phospho-STAT6 (pSTAT6) expression in bronchial asthma (BA). Patients and methods. Eleven patients with allergic (atopic) steroidfree were examined, five healthy controls served as a control. Expression of proteins (STAT6 and pSTAT6) in peripheral blood lymphocytes was studied by Western blot analysis after cell lysis. Preparation of cell lysates and Western blotting were performed using a standard procedure (Amersham). Antibodies against pSTAT6 and STAT6 (manufactured by Cell Signaling) were used. Relative levels of specific proteins were analyzed using actin as a reference, by means of anti-actin antibody. Results. STAT6 phosphorylation was significantly increased in lymphocytes of patients with BA exacerbation, as compared to patients in remission and healthy group. The level of STAT6 was significantly higher compared to healthy persons and showed negative correlation with grade of air flow obstruction. Conclusion. STAT6 and their active form pSTAT6 may play a key role in BA pathophysiology. This study suggests atopic, steroid-free BA (in particular, on exacerbation) to be associated with active cellular inflammatory process, involving activation of STAT6, along with increased level of their active form (pSTAT6). The work was supported by Saint-Petersburg government grants: PD04-4.0-102 (Certificate N ASP604079).V. N. MineevL. N. SorokinaSPb RAACIarticlebronchial asthmajak-stat systemstat 6pstat 6lymphocytesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 9, Iss 4-5, Pp 405-410 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	bronchial asthma jak-stat system stat 6 pstat 6 lymphocytes Immunologic diseases. Allergy RC581-607
spellingShingle	bronchial asthma jak-stat system stat 6 pstat 6 lymphocytes Immunologic diseases. Allergy RC581-607 V. N. Mineev L. N. Sorokina STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
description	Abstract. The aim of present study was to determine the features of STAT6 and phospho-STAT6 (pSTAT6) expression in bronchial asthma (BA). Patients and methods. Eleven patients with allergic (atopic) steroidfree were examined, five healthy controls served as a control. Expression of proteins (STAT6 and pSTAT6) in peripheral blood lymphocytes was studied by Western blot analysis after cell lysis. Preparation of cell lysates and Western blotting were performed using a standard procedure (Amersham). Antibodies against pSTAT6 and STAT6 (manufactured by Cell Signaling) were used. Relative levels of specific proteins were analyzed using actin as a reference, by means of anti-actin antibody. Results. STAT6 phosphorylation was significantly increased in lymphocytes of patients with BA exacerbation, as compared to patients in remission and healthy group. The level of STAT6 was significantly higher compared to healthy persons and showed negative correlation with grade of air flow obstruction. Conclusion. STAT6 and their active form pSTAT6 may play a key role in BA pathophysiology. This study suggests atopic, steroid-free BA (in particular, on exacerbation) to be associated with active cellular inflammatory process, involving activation of STAT6, along with increased level of their active form (pSTAT6). The work was supported by Saint-Petersburg government grants: PD04-4.0-102 (Certificate N ASP604079).
format	article
author	V. N. Mineev L. N. Sorokina
author_facet	V. N. Mineev L. N. Sorokina
author_sort	V. N. Mineev
title	STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_short	STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_full	STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_fullStr	STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_full_unstemmed	STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_sort	stat6 expression by peripheral blood lymphocytes in bronchial asthma
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/90f5e8c9404040918555d453ce63af5e
work_keys_str_mv	AT vnmineev stat6expressionbyperipheralbloodlymphocytesinbronchialasthma AT lnsorokina stat6expressionbyperipheralbloodlymphocytesinbronchialasthma
_version_	1718422558303322112

STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA

Ejemplares similares