Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes

DNA fingerprinting is a major tool in identifying individuals and in evidence matching. However, this technique can be difficult to reproduce in practical classes. Here, we report on distinct PCR profiles obtained when amplifying saliva DNA of a score of distinct individuals with Random Amplified Po...

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Autores principales:	Ana C. Henriques, Paolo De Marco
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/90fed440e2494613a0ae60761b534cbc
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spelling	oai:doaj.org-article:90fed440e2494613a0ae60761b534cbc2021-11-15T15:04:53ZSimple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes10.1128/jmbe.v19i1.13051935-78851935-7877https://doaj.org/article/90fed440e2494613a0ae60761b534cbc2018-03-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v19i1.1305https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885DNA fingerprinting is a major tool in identifying individuals and in evidence matching. However, this technique can be difficult to reproduce in practical classes. Here, we report on distinct PCR profiles obtained when amplifying saliva DNA of a score of distinct individuals with Random Amplified Polymorphic DNA (RAPD)-PCR primer BOXA1R. The RAPD-PCR method is simple and efficient for discrimination between bacterial strains and is used in this instance to obtain personalized fingerprints of each individual’s oral microbiota. We present real results with undergraduate students confirming that this procedure is easily feasible in practical classes. Based on the results presented, we suggest a laboratory activity for undergraduate Molecular Biology or Microbiology students.Ana C. HenriquesPaolo De MarcoAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 19, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Ana C. Henriques Paolo De Marco Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes
description	DNA fingerprinting is a major tool in identifying individuals and in evidence matching. However, this technique can be difficult to reproduce in practical classes. Here, we report on distinct PCR profiles obtained when amplifying saliva DNA of a score of distinct individuals with Random Amplified Polymorphic DNA (RAPD)-PCR primer BOXA1R. The RAPD-PCR method is simple and efficient for discrimination between bacterial strains and is used in this instance to obtain personalized fingerprints of each individual’s oral microbiota. We present real results with undergraduate students confirming that this procedure is easily feasible in practical classes. Based on the results presented, we suggest a laboratory activity for undergraduate Molecular Biology or Microbiology students.
format	article
author	Ana C. Henriques Paolo De Marco
author_facet	Ana C. Henriques Paolo De Marco
author_sort	Ana C. Henriques
title	Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes
title_short	Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes
title_full	Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes
title_fullStr	Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes
title_full_unstemmed	Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes
title_sort	simple protocol for molecular fingerprinting of human oral microbiota samples in lab classes
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/90fed440e2494613a0ae60761b534cbc
work_keys_str_mv	AT anachenriques simpleprotocolformolecularfingerprintingofhumanoralmicrobiotasamplesinlabclasses AT paolodemarco simpleprotocolformolecularfingerprintingofhumanoralmicrobiotasamplesinlabclasses
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Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes

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