Multistable and dynamic CRISPRi-based synthetic circuits

Synthetic circuits based on CRISPRi have not achieved multistable and dynamic behaviors. Here the authors build an oscillator, a toggle switch and an incoherent feed-forward loop using CRISPRi, and provide a mathematical model suggesting that unspecific binding in CRISPRi enables multistability.

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Autores principales:	Javier Santos-Moreno, Eve Tasiudi, Joerg Stelling, Yolanda Schaerli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/91079cbffa374430abcf3eb11b066066
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spelling	oai:doaj.org-article:91079cbffa374430abcf3eb11b0660662021-12-02T15:02:30ZMultistable and dynamic CRISPRi-based synthetic circuits10.1038/s41467-020-16574-12041-1723https://doaj.org/article/91079cbffa374430abcf3eb11b0660662020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16574-1https://doaj.org/toc/2041-1723Synthetic circuits based on CRISPRi have not achieved multistable and dynamic behaviors. Here the authors build an oscillator, a toggle switch and an incoherent feed-forward loop using CRISPRi, and provide a mathematical model suggesting that unspecific binding in CRISPRi enables multistability.Javier Santos-MorenoEve TasiudiJoerg StellingYolanda SchaerliNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Javier Santos-Moreno Eve Tasiudi Joerg Stelling Yolanda Schaerli Multistable and dynamic CRISPRi-based synthetic circuits
description	Synthetic circuits based on CRISPRi have not achieved multistable and dynamic behaviors. Here the authors build an oscillator, a toggle switch and an incoherent feed-forward loop using CRISPRi, and provide a mathematical model suggesting that unspecific binding in CRISPRi enables multistability.
format	article
author	Javier Santos-Moreno Eve Tasiudi Joerg Stelling Yolanda Schaerli
author_facet	Javier Santos-Moreno Eve Tasiudi Joerg Stelling Yolanda Schaerli
author_sort	Javier Santos-Moreno
title	Multistable and dynamic CRISPRi-based synthetic circuits
title_short	Multistable and dynamic CRISPRi-based synthetic circuits
title_full	Multistable and dynamic CRISPRi-based synthetic circuits
title_fullStr	Multistable and dynamic CRISPRi-based synthetic circuits
title_full_unstemmed	Multistable and dynamic CRISPRi-based synthetic circuits
title_sort	multistable and dynamic crispri-based synthetic circuits
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/91079cbffa374430abcf3eb11b066066
work_keys_str_mv	AT javiersantosmoreno multistableanddynamiccrispribasedsyntheticcircuits AT evetasiudi multistableanddynamiccrispribasedsyntheticcircuits AT joergstelling multistableanddynamiccrispribasedsyntheticcircuits AT yolandaschaerli multistableanddynamiccrispribasedsyntheticcircuits
_version_	1718389089887059968

Multistable and dynamic CRISPRi-based synthetic circuits

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