Multistable and dynamic CRISPRi-based synthetic circuits

Synthetic circuits based on CRISPRi have not achieved multistable and dynamic behaviors. Here the authors build an oscillator, a toggle switch and an incoherent feed-forward loop using CRISPRi, and provide a mathematical model suggesting that unspecific binding in CRISPRi enables multistability.

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Bibliographic Details
Main Authors: Javier Santos-Moreno, Eve Tasiudi, Joerg Stelling, Yolanda Schaerli
Format: article
Language:EN
Published: Nature Portfolio 2020
Subjects:
Q
Online Access:https://doaj.org/article/91079cbffa374430abcf3eb11b066066
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