A role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific T cell cytokine expression

Chien-Chang Shen1, Hong-Jen Liang2, Chia-Chi Wang3, Mei-Hsiu Liao4, Tong-Rong Jan1 1Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, 2Innovation and Incubation Center, Yuanpei University, Hsinchu, 3School of Pharmacy, Kaohsi...

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Autores principales: Shen CC, Liang HJ, Wang CC, Liao MH, Jan TR
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Publicado: Dove Medical Press 2011
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spelling oai:doaj.org-article:910c9e920d3041fbb8627ddf6ce3fb922021-12-02T02:42:24ZA role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific T cell cytokine expression1176-91141178-2013https://doaj.org/article/910c9e920d3041fbb8627ddf6ce3fb922011-11-01T00:00:00Zhttp://www.dovepress.com/a-role-of-cellular-glutathione-in-the-differential-effects-of-iron-oxi-a8620https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chien-Chang Shen1, Hong-Jen Liang2, Chia-Chi Wang3, Mei-Hsiu Liao4, Tong-Rong Jan1 1Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, 2Innovation and Incubation Center, Yuanpei University, Hsinchu, 3School of Pharmacy, Kaohsiung Medical University, Kaohsiung, 4Division of Isotope Application, Institute of Energy Research, Taoyuan, Taiwan Background: Accumulating evidence indicates that iron oxide nanoparticles modulate immune responses, and induce oxidative stress in macrophages. It was recently reported that iron oxide nanoparticles attenuated antigen-specific immunity in vivo, though the underlying mechanism remains elusive. The present study investigates the direct effect of iron oxide nanoparticles on antigen-specific cytokine expression by T cells, and potential underlying mechanisms. Methods: Ovalbumin-primed splenocytes were exposed to iron oxide nanoparticles, followed by restimulation with ovalbumin. Cell viability, cytokine production, and cellular levels of glutathione and reactive oxygen species were measured. Results: The splenocyte viability and the production of interleukin-2 and interleukin-4 were unaffected, whereas interferon-γ production was markedly attenuated by iron oxide nanoparticles (10–100 µg iron/mL) in a concentration-dependent manner. Iron oxide nanoparticles also transiently diminished the intracellular level of glutathione, with a peak response at 6 hours posttreatment. The effects of iron oxide nanoparticles on interferon-γ and glutathione were attenuated by the presence of N-acetyl-L-cysteine, a precursor of glutathione. However, iron oxide nanoparticles did not influence the generation of reactive oxygen species. Conclusion: Iron oxide nanoparticles induced a differential effect on antigen-specific cytokine expression by T cells, in which the T helper 1 cytokine IFN-γ was sensitive, whereas the T helper 2 cytokine interleukin-4 was refractory. In addition, the suppressive effect of iron oxide nanoparticles on interferon-γ was closely associated with the diminishment of glutathione. Keywords: iron oxide nanoparticle, T cell, antigen-specific, glutathione, cytokineShen CCLiang HJWang CCLiao MHJan TRDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2791-2798 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Shen CC
Liang HJ
Wang CC
Liao MH
Jan TR
A role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific T cell cytokine expression
description Chien-Chang Shen1, Hong-Jen Liang2, Chia-Chi Wang3, Mei-Hsiu Liao4, Tong-Rong Jan1 1Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, 2Innovation and Incubation Center, Yuanpei University, Hsinchu, 3School of Pharmacy, Kaohsiung Medical University, Kaohsiung, 4Division of Isotope Application, Institute of Energy Research, Taoyuan, Taiwan Background: Accumulating evidence indicates that iron oxide nanoparticles modulate immune responses, and induce oxidative stress in macrophages. It was recently reported that iron oxide nanoparticles attenuated antigen-specific immunity in vivo, though the underlying mechanism remains elusive. The present study investigates the direct effect of iron oxide nanoparticles on antigen-specific cytokine expression by T cells, and potential underlying mechanisms. Methods: Ovalbumin-primed splenocytes were exposed to iron oxide nanoparticles, followed by restimulation with ovalbumin. Cell viability, cytokine production, and cellular levels of glutathione and reactive oxygen species were measured. Results: The splenocyte viability and the production of interleukin-2 and interleukin-4 were unaffected, whereas interferon-γ production was markedly attenuated by iron oxide nanoparticles (10–100 µg iron/mL) in a concentration-dependent manner. Iron oxide nanoparticles also transiently diminished the intracellular level of glutathione, with a peak response at 6 hours posttreatment. The effects of iron oxide nanoparticles on interferon-γ and glutathione were attenuated by the presence of N-acetyl-L-cysteine, a precursor of glutathione. However, iron oxide nanoparticles did not influence the generation of reactive oxygen species. Conclusion: Iron oxide nanoparticles induced a differential effect on antigen-specific cytokine expression by T cells, in which the T helper 1 cytokine IFN-γ was sensitive, whereas the T helper 2 cytokine interleukin-4 was refractory. In addition, the suppressive effect of iron oxide nanoparticles on interferon-γ was closely associated with the diminishment of glutathione. Keywords: iron oxide nanoparticle, T cell, antigen-specific, glutathione, cytokine
format article
author Shen CC
Liang HJ
Wang CC
Liao MH
Jan TR
author_facet Shen CC
Liang HJ
Wang CC
Liao MH
Jan TR
author_sort Shen CC
title A role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific T cell cytokine expression
title_short A role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific T cell cytokine expression
title_full A role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific T cell cytokine expression
title_fullStr A role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific T cell cytokine expression
title_full_unstemmed A role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific T cell cytokine expression
title_sort role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific t cell cytokine expression
publisher Dove Medical Press
publishDate 2011
url https://doaj.org/article/910c9e920d3041fbb8627ddf6ce3fb92
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