Influence of the 5′-terminal sequences on the 5′-UTR structure of HIV-1 genomic RNA

Abstract The 5′-UTR of HIV-1 genomic RNA is known to form specific structures and has important functions. There are three 5′-terminal sequences, G1, G2 and G3, with different localizations in the cell and virion particles as well as different efficiencies in translation and reverse transcription re...

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Autores principales:	Camille Michiko Obayashi, Yoko Shinohara, Takao Masuda, Gota Kawai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/910ca2dc39bd448a8b750b7f99ccaceb
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spelling	oai:doaj.org-article:910ca2dc39bd448a8b750b7f99ccaceb2021-12-02T15:00:19ZInfluence of the 5′-terminal sequences on the 5′-UTR structure of HIV-1 genomic RNA10.1038/s41598-021-90427-92045-2322https://doaj.org/article/910ca2dc39bd448a8b750b7f99ccaceb2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90427-9https://doaj.org/toc/2045-2322Abstract The 5′-UTR of HIV-1 genomic RNA is known to form specific structures and has important functions. There are three 5′-terminal sequences, G1, G2 and G3, with different localizations in the cell and virion particles as well as different efficiencies in translation and reverse transcription reactions. In the present study, the structural characteristics of the joint region between the TAR and PolyA stems was analysed, and it was found that small differences in the 5′-terminus affect the conformational characteristics of the stem-loop structures. In the G1 form, the two stems form a coaxial stem, whereas in the G2 and G3 forms, the two stems are structurally independent of each other. In the case of the G1 form, the 3′-flanking nucleotides of the PolyA stem are included in the stable coaxial stem structure, which may affect the rest of the 5′-UTR structure. This result demonstrates that the local conformation of this functionally key region has an important role in the function of the 5′-UTR.Camille Michiko ObayashiYoko ShinoharaTakao MasudaGota KawaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Camille Michiko Obayashi Yoko Shinohara Takao Masuda Gota Kawai Influence of the 5′-terminal sequences on the 5′-UTR structure of HIV-1 genomic RNA
description	Abstract The 5′-UTR of HIV-1 genomic RNA is known to form specific structures and has important functions. There are three 5′-terminal sequences, G1, G2 and G3, with different localizations in the cell and virion particles as well as different efficiencies in translation and reverse transcription reactions. In the present study, the structural characteristics of the joint region between the TAR and PolyA stems was analysed, and it was found that small differences in the 5′-terminus affect the conformational characteristics of the stem-loop structures. In the G1 form, the two stems form a coaxial stem, whereas in the G2 and G3 forms, the two stems are structurally independent of each other. In the case of the G1 form, the 3′-flanking nucleotides of the PolyA stem are included in the stable coaxial stem structure, which may affect the rest of the 5′-UTR structure. This result demonstrates that the local conformation of this functionally key region has an important role in the function of the 5′-UTR.
format	article
author	Camille Michiko Obayashi Yoko Shinohara Takao Masuda Gota Kawai
author_facet	Camille Michiko Obayashi Yoko Shinohara Takao Masuda Gota Kawai
author_sort	Camille Michiko Obayashi
title	Influence of the 5′-terminal sequences on the 5′-UTR structure of HIV-1 genomic RNA
title_short	Influence of the 5′-terminal sequences on the 5′-UTR structure of HIV-1 genomic RNA
title_full	Influence of the 5′-terminal sequences on the 5′-UTR structure of HIV-1 genomic RNA
title_fullStr	Influence of the 5′-terminal sequences on the 5′-UTR structure of HIV-1 genomic RNA
title_full_unstemmed	Influence of the 5′-terminal sequences on the 5′-UTR structure of HIV-1 genomic RNA
title_sort	influence of the 5′-terminal sequences on the 5′-utr structure of hiv-1 genomic rna
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/910ca2dc39bd448a8b750b7f99ccaceb
work_keys_str_mv	AT camillemichikoobayashi influenceofthe5terminalsequencesonthe5utrstructureofhiv1genomicrna AT yokoshinohara influenceofthe5terminalsequencesonthe5utrstructureofhiv1genomicrna AT takaomasuda influenceofthe5terminalsequencesonthe5utrstructureofhiv1genomicrna AT gotakawai influenceofthe5terminalsequencesonthe5utrstructureofhiv1genomicrna
_version_	1718389214967496704

Influence of the 5′-terminal sequences on the 5′-UTR structure of HIV-1 genomic RNA

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