An AgNP-deposited commercial electrochemistry test strip as a platform for urea detection

Abstract We developed an inexpensive, portable platform for urea detection via electrochemistry by depositing silver nanoparticles (AgNPs) on a commercial glucose test strip. We modified this strip by first removing the enzymes from the surface, followed by electrodeposition of AgNPs on one channel...

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Autores principales:	Juanjuan Liu, Roozbeh Siavash Moakhar, Ayyappasamy Sudalaiyadum Perumal, Horia Nicolae Roman, Sara Mahshid, Sebastian Wachsmann-Hogiu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/910cf3e8f24e4231904570fcf8ae4def
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spelling	oai:doaj.org-article:910cf3e8f24e4231904570fcf8ae4def2021-12-02T17:52:33ZAn AgNP-deposited commercial electrochemistry test strip as a platform for urea detection10.1038/s41598-020-66422-x2045-2322https://doaj.org/article/910cf3e8f24e4231904570fcf8ae4def2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66422-xhttps://doaj.org/toc/2045-2322Abstract We developed an inexpensive, portable platform for urea detection via electrochemistry by depositing silver nanoparticles (AgNPs) on a commercial glucose test strip. We modified this strip by first removing the enzymes from the surface, followed by electrodeposition of AgNPs on one channel (working electrode). The morphology of the modified test strip was characterized by Scanning Electron Microscopy (SEM), and its electrochemical performance was evaluated via Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). We evaluated the performance of the device for urea detection via measurements of the dependency of peak currents vs the analyte concentration and from the relationship between the peak current and the square root of the scan rates. The observed linear range is 1–8 mM (corresponding to the physiological range of urea concentration in human blood), and the limit of detection (LOD) is 0.14 mM. The selectivity, reproducibility, reusability, and storage stability of the modified test strips are also reported. Additional tests were performed to validate the ability to measure urea in the presence of confounding factors such as spiked plasma and milk. The results demonstrate the potential of this simple and portable EC platform to be used in applications such as medical diagnosis and food safety.Juanjuan LiuRoozbeh Siavash MoakharAyyappasamy Sudalaiyadum PerumalHoria Nicolae RomanSara MahshidSebastian Wachsmann-HogiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juanjuan Liu Roozbeh Siavash Moakhar Ayyappasamy Sudalaiyadum Perumal Horia Nicolae Roman Sara Mahshid Sebastian Wachsmann-Hogiu An AgNP-deposited commercial electrochemistry test strip as a platform for urea detection
description	Abstract We developed an inexpensive, portable platform for urea detection via electrochemistry by depositing silver nanoparticles (AgNPs) on a commercial glucose test strip. We modified this strip by first removing the enzymes from the surface, followed by electrodeposition of AgNPs on one channel (working electrode). The morphology of the modified test strip was characterized by Scanning Electron Microscopy (SEM), and its electrochemical performance was evaluated via Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). We evaluated the performance of the device for urea detection via measurements of the dependency of peak currents vs the analyte concentration and from the relationship between the peak current and the square root of the scan rates. The observed linear range is 1–8 mM (corresponding to the physiological range of urea concentration in human blood), and the limit of detection (LOD) is 0.14 mM. The selectivity, reproducibility, reusability, and storage stability of the modified test strips are also reported. Additional tests were performed to validate the ability to measure urea in the presence of confounding factors such as spiked plasma and milk. The results demonstrate the potential of this simple and portable EC platform to be used in applications such as medical diagnosis and food safety.
format	article
author	Juanjuan Liu Roozbeh Siavash Moakhar Ayyappasamy Sudalaiyadum Perumal Horia Nicolae Roman Sara Mahshid Sebastian Wachsmann-Hogiu
author_facet	Juanjuan Liu Roozbeh Siavash Moakhar Ayyappasamy Sudalaiyadum Perumal Horia Nicolae Roman Sara Mahshid Sebastian Wachsmann-Hogiu
author_sort	Juanjuan Liu
title	An AgNP-deposited commercial electrochemistry test strip as a platform for urea detection
title_short	An AgNP-deposited commercial electrochemistry test strip as a platform for urea detection
title_full	An AgNP-deposited commercial electrochemistry test strip as a platform for urea detection
title_fullStr	An AgNP-deposited commercial electrochemistry test strip as a platform for urea detection
title_full_unstemmed	An AgNP-deposited commercial electrochemistry test strip as a platform for urea detection
title_sort	agnp-deposited commercial electrochemistry test strip as a platform for urea detection
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/910cf3e8f24e4231904570fcf8ae4def
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An AgNP-deposited commercial electrochemistry test strip as a platform for urea detection

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